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[1]王伟,姜伟,张金龙,等.大豆种质的耐旱性鉴定及耐旱指标筛选[J].大豆科学,2015,34(05):808-818.[doi:10.11861/j.issn.1000-9841.2015.05.0808]
　WANG Wei,JIANG Wei,ZHANG Jin-long,et al.Selection of Drought-tolerant Soybean and Evaluation of the Drought.tolerance Indices[J].Soybean Science,2015,34(05):808-818.[doi:10.11861/j.issn.1000-9841.2015.05.0808]

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大豆种质的耐旱性鉴定及耐旱指标筛选

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第34卷期数: 2015年05期页码: 808-818 栏目: 出版日期: 2015-10-25

Title:: Selection of Drought-tolerant Soybean and Evaluation of the Drought.tolerance Indices

作者:: 王伟; 姜伟; 张金龙; 苗龙; 赵团结; 盖钧镒; 李艳; 南京农业大学大豆研究所/国家大豆改良中心/作物遗传与种质创新国家重点试验室/农业部大豆生物学与遗传育种重点试验室，江苏南京 210095

Author(s):: WANG Wei; JIANG Wei; ZHANG Jin-long; MIAO Long; ZHAO Tuan-jie; GAI Jun-yi; LI Yan; Soybean Research Institute of Nanjing Agricultural University/National Center for Soybean Improvement/National Key Laboratory of Crop Genetics and Germplasm Enhancement/MOA Key Laboratory for Biology and Genetic Improvement of Soybean (General), Nanjing 210095, China

关键词:: 大豆; 干旱; 耐旱指标

Keywords:: Soybean; Drought; Drought-tolerance indices

DOI:: 10.11861/j.issn.1000-9841.2015.05.0808

文献标志码:: A

摘要:: 干旱是影响大豆产量的重要因素之一，建立相对稳定的耐旱鉴定方法、发掘耐旱品种是大豆耐旱育种的前提。基于前期对87份大豆材料耐旱性的初步鉴定结果以及对其中30份大豆材料耐旱等级划分重复鉴定的基础上，选取了代表性大豆材料12份，对分别反映萎蔫情况、叶片相对含水量、生长状况的10个耐旱相关指标进行测定。结果表明：与对照相比，干旱胁迫下大豆的叶片相对含水量降低，叶片相对电导率升高，大部分材料的生长受到抑制，生物量降低，根冠比增加，以上指标在不同材料间均存在显著差异(P＜0.05)。萎蔫天数的遗传率为93%，是简单、直观、稳定的耐旱鉴定指标。相关性分析表明：萎蔫等级、叶片相对电导率分别与萎蔫天数呈极显著负相关，叶片相对含水量、根相对干重分别与萎蔫天数呈极显著正相关，且这些指标具有较高的遗传率(73%～89%)，亦可作为耐旱鉴定的指标。筛选到的PI595843、科丰1号、五河齐黄豆等耐旱种质可为大豆耐旱育种提供材料基础。

Abstract:: Drought is one of the major factors that cause soybean yield loss.Selection of the drought-tolerant soybean varieties and establishment of a relatively stable evaluation method for drought-tolerance is important for soybean drought-tolerance breeding.In this research, the drought-tolerance of soybean germplasm and the drought-tolerance indices were evaluated in two sand-culturing experiments.Twelve soybean varieties were selected after screening 87 and rescreening 30 soybean varieties using the drought-tolerance score, and 10 drought-tolerance indices reflecting soybean leaf wilting, leaf water content, and biomass were evaluated.Compared with the normal water regime, the leaf relative water content (RWC) decreased while the relative electrical conductivity (REC) in soybean leaves increased, and the growth rate and biomass of most soybean varieties decreased while the root to shoot ratio (R/S) increased under drought stress.There was significant difference among soybean varieties using these indices (P＜0.05). Days-to-wilt (DTW) has a heritability of 93% and is a simple and relatively stable index. There was a significantly negative correlation of the wilting score and REC with DTW, respectively, and a significantly positive correlation of RWC and the relative root dry weight with DTW, respectively.Furthermore, these indices had a relative high heritability of 73% to 89%, which could be used as additional selection indices for soybean drought-tolerance.Three soybean varieties, including PI595843, KF-1, and Wuheqihuangdou showed strong drought tolerance in this research, which could be used in future soybean drought-tolerance breeding program.

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备注/Memo

基金项目：教育部长江学者和创新团队发展计划（PCSIRT13073）；农业部大豆生物学与遗传育种创新团队；中央高校基本科研业务费专项资金；江苏省双创计划。

第一作者简介：王伟（1988-），男，博士，主要从事大豆遗传育种研究。Email:wangwei2013201046@163.com。

通讯作者：李艳（1975-），女，博士，教授，主要从事大豆遗传育种及基因组学研究。Email: yanli1@njau.edu.cn。

更新日期/Last Update: 2015-11-07