[1]钟鹏,吴俊江,刘丽君,等.干旱胁迫对不同磷效率基因型大豆膜脂过氧化作用的影响[J].大豆科学,2008,27(04):610-615.[doi:10.11861/j.issn.1000-9841.2008.04.0610]
　ZHONG-Peng,WU Jun-jiang,LIU Li-jun,et al.Effect of Drought Stress on Lipid Peroxidation in Soybean Varieties with Different P Efficiency[J].Soybean Science,2008,27(04):610-615.[doi:10.11861/j.issn.1000-9841.2008.04.0610]
点击复制

干旱胁迫对不同磷效率基因型大豆膜脂过氧化作用的影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第27卷 期数: 2008年04期 页码: 610-615 栏目: 出版日期: 2008-08-25
Title:
Effect of Drought Stress on Lipid Peroxidation in Soybean Varieties with Different P Efficiency
文章编号:
1000-9841(2008)04-0610-06
作者:
钟鹏1; 吴俊江1; 刘丽君1; 林蔚刚1; 董德建1; 王建丽2
1黑龙江省农业科学院大豆研究所；
2黑龙江省农业科学院草业研究所，黑龙江 哈尔滨 150086
Author(s):
ZHONG-Peng1; WU Jun-jiang1; LIU Li-jun1; LIN Wei-gang1; DONG De-jian1; WANG Jian-li2
1 Soybean Research Institute，Heilongjiang Academy of Agricultural Sciences，Harbin 150086；
2 Institute of Pratacultural Sciences，Heilongjiang Academy of Agricultural Sciences，Harbin 150086，Heilongjiang，China
关键词:
大豆; 干旱; 磷效率; 膜脂过氧化
Keywords:
Soybean; Drought stress; Phosphorus efficiencies; Lipid peroxidation
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2008.04.0610
文献标志码:
A
摘要:
以不同磷效率基因型大豆黑河27、黑河29为材料，在控制磷素供应水平的条件下，通过控制灌水量的盆栽试验，研究了干旱胁迫对不同磷效率基因型大豆膜脂过氧化作用的影响。结果表明：随土壤干旱强度增加，磷高效基因型大豆叶片丙二醛(MDA)含量、细胞膜透性和O.2含量递增的幅度明显小于磷低效基因型，超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性及过氧化物酶(POD)活性降低的幅度显著小于磷低效基因型，渗透调节物质可溶性糖（SS）、脯氨酸(Pro)随干旱胁迫强度增大而增加，磷高效基因型大豆的渗透调节能力要明显大于磷低效基因型。表明大豆耐旱性与其抗氧化酶活性相关，渗透调节物质是构成其耐旱性的重要物质基础，磷高效基因型膜脂过氧化程度小于磷低效基因型，并且表现出显著性差异。
Abstract:
Soybean cultivars with high phosphorus efficiency adapt to P stress environment easily.Previous studies have focused on the mechanism of soybean’s adaptation to low P stress.But the coupling effect of water and phosphorus on different P efficiency genotypic soybean is less documented.Therefore，pot experiments were conducted in 2007 year，with P high efficient soybean Heihe 27 and P low efficient soybean Heihe 29 as materials，to determine the influence of water and P coupling on lipid peroxidation characters of different genotypic soybean at seedling stage.Two P（applied P and without P)and four water（100%，70%，50% and 30% of the maximum field water holding capability)treatments were designed and endosmosis permeability，O2-.content，malondiaidehyde（MDA)content，relative conductivity，Superoxide dismutase（SOD)，peroxide enzyme（POD)activity，catalase enzyme（CAT)activity，proline content，soluble sugar content were determined.As drought stress increased，the MDA，relative conductivity and O2-content of P high efficient soybean increased and the increasing scope was obviously smaller than that of P low efficient soybean；the decrease scope of SOD，POD and CAT activity of P high efficient soybean was remarkably smaller than that of P low efficient soybean; proline and soluble sugar content of P high efficient soybean increased obviously.The results indicate that antioxidant enzymes are related to drought tolerance and the osmotic adjustment materials are the inherent basis of superior drought tolerance of different phosphorus efficient soybean，and the lipid peroxidation degree of P high efficient soybean is significantly reduced.

参考文献/References:

[1] Bowler C，Van Montagu M，Inze D.Superoxide dismutase and stress tolerance[J].Annual Reviews in Plant Physiology and Plant Molecular，1992,43：83-116

[2]Fridovich I.Superoxide dismutase[J].Annual Reviews in Biochemistry，1975，44：147-159.
[3]余叔文，汤章城.植物生理与分子生物学[M].北京:科学出版社，1999:366-382.(Yu S W，Tang Z C.Plant physiology and molecular biology[M].Bejing：Science Press，1999:366-382.)
[4]汤章城,王育启,吴亚华,等.不同抗旱品种高粱苗中脯氨酸累积的差异[J].植物生理学报，1986,12(2):154-162.(Tang Z C，Wang Y Q，Wu Y H，et al.The difference in praline accumulation between the seedlings of two varieties of sorghum with different drought resistance[J].Acta Phytophysiologica Sinica,1986,12(2):154-162.)
[5]王庆仁，李继云，李振声.植物对土壤磷素利用效率的研究[J].生态学报，1999,19(3)：417-421.(Wang Q R，Li J Y，Li Z S.Studies on plant nutrition of efficient utility for soil phosphorus[J].Acta Ecologica Sinica,1999,19(3)：417-421.)
[6]赵其国.中国东部红壤地区土壤退化的时空变化、机理及调控[M].北京：科学出版社，2002：142-146.（Zhao Q G.Change and control of degraded soil of red soil region of east China[M].Beijing：Science Press，2002:142-146.）
[7]梁银丽，陈培元.土壤水分和氮磷营养对小麦根系生理特性的影响[J].植物生态学报，1996,(20):255-262.(Liang Y L，Chen P Y .Adjustment of physiological characters of root system of wheat by soil moisture and nitrogen and phosphorus[J].Acta Phytoecologica Sinica，1996,(20):255-262.)
[8]Rodriguez D，Goudriaan J，Oyarzabal M，et al.Phosphorus nutrition and water stress tolerance in wheat plants[J].Journal of Plant Nutrition，1996，19:29-39.
[9]沈玉芳，王保莉，曲东.水分胁迫下磷营养对玉米苗期根系导水率的影响[J].西北农林科技大学学报，2002,30(5):11-15.(Shen Y F，Wang B L，Qu D.Effects of phosphorus on root hydraulic conductivity of corn under water stress[J].Journal of North west Sci-Tech University of Agriculture and Forestry，2002,30(5):11-15.)
[10]刘厚诚，邝炎华，陈日远.缺磷胁迫下长豇豆幼苗膜脂过氧化及保护酶活性的变化[J].园艺学报，2003,30（2）：215-217.(Liu H C，Kuang Y H，Chen R Y.Changes of lipid peroxidation and activities of protective enzymes in asparagus bean seedling sunder phosphorus-deficiency stress[J].Acta Horticulturae Sinica，2003,30(2):215-217.)
[11]潘晓华,刘水英,李锋，等.低磷胁迫对不同水稻品种叶片膜质过氧化及保护酶活性的影响[J].中国水稻科学，2003，17(1):57-60.(Pan X H，Liu S Y，Li F，et al.Effect of low-phosphorus stress on membrane lipid peroxidation and protective enzyme activities in rice leaves of different cultivars[J].Chinese Journal of Rice Science，2003,17(1):57-60.)
[12]张宪政.植物生理学实验指导[M].北京:中国农业出版社，2000:5-96.(Zhang X Z.Guide of phytophysiological experiment[M].Beijing：Agricultural Press，2000:5-96.)
[13]张志良.植物生理学实验指导[M].北京:中国农业出版社，1990:5-8.(Zhang Z L.A Guide of phytophysiological experiment[M].Beijing：Higher Education Press，1990:5-8.)
[14]邹琦.植物生理学实验指导[M].北京:高等教育出版社，2000:59-60,173-174.(ZouQ.Guideof Phytophysiological Experiment[M].Beijing：Agricultural Press，2000:59-60,173-174.)
[15]Tuner N C .Concurrent comparisons of stomata behavior，water status，and evaporation of maize in soil at high or low water potential[J].Plant Physiology，1990，(55):932-936.
[16]蒋明义,郭绍川.水分亏缺诱导的氧化胁迫和植物的抗氧化作用[J].植物生理学通讯,1996,32(2):144-150.(Jiang M Y，Guo S C.Oxidative stresses and antioxidantion induced by water deficiency in plants[J].Plant Physiology Communication，1996,32(2):144-150.)
[17]张木清,陈如凯,于松烈.水分胁迫下蔗叶活性氧代谢的数学分析[J].作物学报，1996,22(6):729-735.(Zhang M Q，Chen R Q，Yu S L.Mathematics analysis for the activity oxygen metabolism in the drought stress leaves of sugar cane[J].Acta Agronomica Sinica，1996,22(6):729-735.)
[18]万美亮，邝炎华，陈建勋.缺磷胁迫对甘蔗膜脂过氧化和保护酶活性的影响[J].华南农业大学学报，1999,2(2):58-61.(Wan M L，Kuang Y H，Chen J X.Effect of phosphorus defection membrane-lipid peroxidation and the activity of defense enzymes systems in sugar cane[J].Journal of South China Agricultural University,1999,2(2):58-61.)

相似文献/References:

[1]刘章雄,李卫东,孙石,等.1983～2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
　LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(04):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
　LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(04):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
　WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(04):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
　ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(04):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
　LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(04):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
　DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(04):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
　ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(04):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
　GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(04):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
　WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(04):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
　LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(04):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
[11]闫春娟,王文斌,涂晓杰,等.不同生育时期干旱胁迫对大豆根系特性及产量的影响[J].大豆科学,2013,32(01):59.[doi:10.3969/j.issn.1000-9841.2013.01.014]
　YAN Chun-juan,WANG Wen-bin,TU Xiao-jie,et al.Effect of Drought Stress at Different Growth Stage on Yield and Root Characteristics of Soybean[J].Soybean Science,2013,32(04):59.[doi:10.3969/j.issn.1000-9841.2013.01.014]
[12]刘颖,张明怡,韩光,等.干旱胁迫下钾对大豆叶片保护酶活性及产量的影响[J].大豆科学,2011,30(02):341.[doi:10.11861/j.issn.1000-9841.2011.02.0341]
　LIU Ying,ZHANG Ming-yi,HAN Guang,et al.Effect of Potassium on Soybean Leaf Protective Enzymes and Yield under Drought Stress[J].Soybean Science,2011,30(04):341.[doi:10.11861/j.issn.1000-9841.2011.02.0341]
[13]王伟,姜伟,张金龙,等.大豆种质的耐旱性鉴定及耐旱指标筛选[J].大豆科学,2015,34(05):808.[doi:10.11861/j.issn.1000-9841.2015.05.0808]
　WANG Wei,JIANG Wei,ZHANG Jin-long,et al.Selection of Drought-tolerant Soybean and Evaluation of the Drought.tolerance Indices[J].Soybean Science,2015,34(04):808.[doi:10.11861/j.issn.1000-9841.2015.05.0808]
[14]魏崃,吴广锡,唐晓飞,等.过表达GmHSFA1大豆在干旱条件下对高温的响应[J].大豆科学,2016,35(02):257.[doi:10.11861/j.issn.1000-9841.2016.02.0257]
　WEI Lai,WU Guang-xi,TANG Xiao-fei,et al.Soybean Responses to High Temperatures Under Drought Stress in the Presence of An Over-expressed GmHSFA1 Gene[J].Soybean Science,2016,35(04):257.[doi:10.11861/j.issn.1000-9841.2016.02.0257]
[15]金毅,郑浩宇,金喜军,等.外源ABA、SA及JA对干旱胁迫及复水下大豆生长的影响[J].大豆科学,2016,35(06):958.[doi:10.11861/j.issn.1000-9841.2016.06.0958]
　JIN Yi,ZHENG Hao-yu,JIN Xi-jun,et al.Effect of ABA，SA and JA on Soybean Growth Under Drought Stress and Re-watering[J].Soybean Science,2016,35(04):958.[doi:10.11861/j.issn.1000-9841.2016.06.0958]
[16]刘丽君,尹田夫,孟良.大豆原生质膜及混合细胞器膜脂脂肪酸对干旱胁迫的反应[J].大豆科学,1991,10(01):46.[doi:10.11861/j.issn.1000-9841.1991.01.0046]
　[J].Soybean Science,1991,10(04):46.[doi:10.11861/j.issn.1000-9841.1991.01.0046]
[17]谢甫绨,董钻,赵艺新.大豆器官间的热能分布与耐旱性的关系初报[J].大豆科学,1993,12(02):107.[doi:10.11861/j.issn.1000-9841.1993.02.0107]
　[J].Soybean Science,1993,12(04):107.[doi:10.11861/j.issn.1000-9841.1993.02.0107]
[18]刘莎莎,柏新富,冯春晓,等.干旱条件下土壤盐分对大豆生长及光合作用的影响[J].大豆科学,2017,36(06):921.[doi:10.11861/j.issn.1000-9841.2017.06.0921]
　LIU Sha-sha,BAI Xin-fu,FENG Chun-xiao,et al.Effects of Soil Salinity on the Growth and Photosynthesis of Soybean under Drought Conditions[J].Soybean Science,2017,36(04):921.[doi:10.11861/j.issn.1000-9841.2017.06.0921]
[19]邹京南,金喜军,王孟雪,等.外源褪黑素对干旱胁迫条件下大豆苗期光合及生理的影响[J].大豆科学,2018,37(06):896.[doi:1011861/jissn1000-98412018060896]
　ZOU Jing-nan,JIN Xi-jun,WANG Meng-xue,et al.Effects of Exogenous Melatonin on Photosynthesis and Physiology of Soybean Seedlings under Drought Stress[J].Soybean Science,2018,37(04):896.[doi:1011861/jissn1000-98412018060896]
[20]李清,王洪,郭禄芹,等.大豆Whirly基因家族的鉴定和表达分析[J].大豆科学,2019,38(02):204.[doi:10.11861/j.issn.1000-9841.2019.02.0204]
　LI Qing,WANG Hong,GUO Lu-qin,et al.Identification and Expression Analysis of the Whirly Gene Family in Soybean[J].Soybean Science,2019,38(04):204.[doi:10.11861/j.issn.1000-9841.2019.02.0204]

备注/Memo

基金项目：黑龙江省“十一五”科技攻关资助项目（GA06B101-2-5，GA06B101-1-2）；“十一五”国家科技支撑计划资助项目（2006BAD21B01-6）；黑龙江省自然科学基金资助项目（C2007-13）；农业部948项目子课题。

作者简介：钟鹏（1978-），男，硕士，助理研究员，主要从事大豆高产栽培理论与技术研究。E-mail: zhongpengvip@163.com。

更新日期/Last Update: 2014-10-07