[1]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43-49.[doi:10.3969/j.issn.1000-9841.2013.01.010]
　WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(01):43-49.[doi:10.3969/j.issn.1000-9841.2013.01.010]
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适于体细胞胚发生的大豆基因型筛选

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第32卷 期数: 2013年01期 页码: 43-49 栏目: 出版日期: 2013-02-25
Title:
Screening of Soybean Genotypes Suitable for Somatic Embryogenesis
文章编号:
1000-9841(2013)01-0043-03
作者:
王鹏飞1; 2; 刘丽君2; 唐晓飞2; 魏崃2; 吴广锡2; 王兴宇1; 2
1.东北农业大学 生命科学学院，黑龙江 哈尔滨 150030；
2.黑龙江省农业科学院 大豆研究所，黑龙江 哈尔滨 150086
Author(s):
WANG Peng-fei1; 2;  LIU Li-jun2; TANG Xiao-fei2; WEI Lai2; WU Guang-xi2; WANG Xing-yu1; 2
1.College of Life Sciences,Northeast Agricultural University,Harbin 150030,Heilongjiang;
2.Soybean Research Institute,Heilongjiang Academy of Agricultural Sciences,Harbin 150086,Heilongjiang,China
关键词:
大豆; 基因型; 体细胞胚; 筛选
Keywords:
Soybean; Genotype; Somatic embryos; Screening
分类号:
S565.1
DOI:
10.3969/j.issn.1000-9841.2013.01.010
摘要:
以41个北方春大豆品种为材料,研究了不同基因型大豆体细胞胚的诱导率。结果表明:不同基因型大豆的体细胞胚发生率有显著差异,在供试品种中,垦丰23的体细胞胚胎发生率(99.7%)最高,合丰55等9个基因型体细胞胚诱导率为70%～90%;登科4等5个基因型无体细胞胚发生。垦丰23体细胞胚呈葡萄状、颜色鲜绿、每个未成熟子叶上体细胞胚的数量较多、无畸形胚、且每个体细胞胚是独立存在的,其他诱导率较高的基因型每个未成熟子叶分化体细胞胚的数目较少,且部分基因型含有畸形胚。
Abstract:
Genotype is one of the important factors influencing soybean somatic embryo induction and plant regeneration.Forty one spring soybean cultivars were researched to screen the ideal genotype for somatic embryo induction.The frequencies of soybean somatic embryo was different among tested materials,Kenfeng 23(99.7%) was the highest;nine genotypes,including Hefeng 55,ranged from seventy to ninety percent;five genotypes,including Dengke 4,had no somatic embryos.The somatic embryos of Kenfeng 23 were grape-like and in bright green color.Kenfeng 23,with great number of somatic embryo on each immature cotyledon,did not produce deformity embryos,furthermore,each somatic embryo existed independently.For the other high inductivity cultivars,the somatic embryos number of each immature cotyledon was less than Kenfeng 23,and some cultivars had deformity embryos.

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备注/Memo

基金项目：国家转基因重大专项(2011ZX08004-002)；国家科技支撑计划 (2011BAD35B06-1-4)；大豆产业技术体系(CARS004-PS05)。

第一作者简介：王鹏飞(1988-)，女，在读硕士，研究方向为分子遗传学。E-mail:wangpengfei_885@126.com。
通讯作者：刘丽君(1958-)，女，研究员，从事大豆遗传育种的研究。E-mail:nkyssbd@126.com。

更新日期/Last Update: 2013-02-20