PDF下载 +分享

微信公众号：大豆科学

[1]邹京南,金喜军,王孟雪,等.外源褪黑素对干旱胁迫条件下大豆苗期光合及生理的影响[J].大豆科学,2018,37(06):896-905.[doi:1011861/jissn1000-98412018060896]
　ZOU Jing-nan,JIN Xi-jun,WANG Meng-xue,et al.Effects of Exogenous Melatonin on Photosynthesis and Physiology of Soybean Seedlings under Drought Stress[J].Soybean Science,2018,37(06):896-905.[doi:1011861/jissn1000-98412018060896]

点击复制

外源褪黑素对干旱胁迫条件下大豆苗期光合及生理的影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第37卷期数: 2018年06期页码: 896-905 栏目: 出版日期: 2018-11-20

Title:: Effects of Exogenous Melatonin on Photosynthesis and Physiology of Soybean Seedlings under Drought Stress

作者:: 邹京南; 金喜军; 王孟雪; 张明聪; 任春元; 于奇; 胡国华; 张玉先; （黑龙江八一农垦大学农学院，黑龙江大庆 163319）

Author(s):: ZOU Jing-nan; JIN Xi-jun; WANG Meng-xue; ZHANG Ming-cong; REN Chun-yuan; YU Qi; HU Guo-hua; ZHANG Yu-xian; （Agronomy of College, Heilongjiang Bayi Agricultural University, Daqing 163319, China）

关键词:: 大豆; 干旱; 褪黑素; 光合作用; 膜脂过氧化

Keywords:: Soybean; Drought; Melatonin; Photosynthesis; Membrane lipid peroxidation

DOI:: 1011861/jissn1000-98412018060896

文献标志码:: A

摘要:: 为研究外源褪黑素对干旱胁迫下大豆的缓解作用，促进褪黑素在生产实际中的应用，本试验以大豆抗线9为供试品种，采用盆栽控水的方式模拟干旱胁迫，设置不同浓度褪黑素叶面喷施处理，研究外源褪黑素对干旱胁迫下大豆幼苗生长的调控效应，为实际生产提供理论基础和技术参考。结果表明：外源褪黑素可显著缓解干旱胁迫对大豆幼苗生长的抑制作用，与干旱胁迫相比，干旱胁迫下喷施100和200 μmol·L^-1褪黑素处理促进叶面积和干物质积累总量，分别平均提高了804%和905%、676%和932%、1389%和1120%；显著提高了叶片光合参数、叶绿素荧光参数和相对含水量，其中Pn在第7和第14天分别提高了2288%和5457%，3972%和5753%，Tr分别提高了3701%和1899%，5965%和4517%；提高了抗氧化酶活性和渗透调节物质含量，降低了膜脂过氧化水平，其中SOD活性分别提高了4173%和2351%，1977%和1518%，MDA分别降低了3747%和2629%，3368%和1925%。综上所述，外源褪黑素可以提高干旱胁迫下大豆幼苗光合参数和叶绿素荧光参数，提高抗氧化酶活性进而促进ROS的清除，提高渗透调节物质含量有利于叶片保持较高的相对含水量，降低膜脂过氧化程度，从而提高大豆幼苗耐旱能力。

Abstract:: In order to study the alleviation effect of exogenous melatonin on soybean under drought stress, promoted the application of melatonin in production, this experiment used soybean ［Glycine max ［L］ Merr］ kang-xian 9 as the material by pot The effects of 100 and 200 μmol·L-1 exogenous melatonin on the photosynthesis, antioxidant system and growth by 30% field capacity under drought stress and rewatered soybean seedlings were studied The results showed that foliar application of melatonin under drought stress could alleviate the growth inhibition, leaf area index and dry matter caused by drought stress, 100 and 200 μmol·L-1 were more drought-treated, the stress plant height, leaf area and total dry matter increased by 804% and 905%, 676% and 932%, 1389% and 1120%, respectively Pn increased by 2288% and 5457%, 3972% and 5753%, respectively on 7th day and 14th day, and Tr increased by 3701% and 1899%, 5965% and 4517%, respectively, other photosynthesis, chlorophyll fluorescence and RWC were consistent, and also enhanced antioxidant enzyme activity and osmotic adjustment substances, reduced membrane lipid peroxidation level and better effect under rehydration conditions, where in SOD activity increased by 4173% and 2351%, 1977% and 1518%, respectively, and MDA decreased by 3747% and 2629%, 3368% and 1925%, respectively The other antioxidant enzyme activities, osmotic adjustment substances and membrane lipid peroxidation levels were consistent In summary, exogenous melatonin can improved photosynthetic parameters and chlorophyll fluorescence parameters under drought stress, increased antioxidant enzyme activity, clear ROS and reduced membrane lipid peroxidation, and improved osmotic adjustment substances to maintain higher RWC of leaves, to improved the drought tolerance of soybean seedlings

参考文献/References:

［1］高鑫宇，刘丽君，刘博，等 PEG模拟干旱对大豆抗氧化酶活性及抗氧化能力的影响［J］. 大豆科学，2016，35 (4)：616-619,636 (Gao X Y，Liu L J，Liu B，et al. Effect of drought stress simulated by PEG on antioxidant enzyme activities and antioxidant capacity in soybean［J］. Soybean Science，2016，35 (4)：616-619,636)

［2］郭数进，杨凯敏，霍瑾，等干旱胁迫对大豆鼓粒期叶片光合能力和根系生长的影响［J］. 应用生态学报，2015，26 (5)：1419-1425 (Guo S J，Yang K M，Huo J，et al. Influence of drought on leaf photosynthetic capacity and root growth of soybeans at grain filling stage［J］. Chinese Journal of Applied Ecology，2015，26 (5)：1419-1425)

［3］李建英，周长军，杨柳，等水分胁迫对大豆苗期叶片内源激素含量与保护酶活性的影响［J］. 大豆科学，2010，29 (6)：959-963 (Li J Y，Zhou C J，Yang L，et al. Effects of water stress on endogenous hormone contents and protective enzyme activities in leaves of soybean seedlings［J］. Soybean Science，2010，29 (6)：959-963)

［4］Woo H R，Kim J H，Nam H G，et al. The delayed leaf senescence mutants of Arabidopsis，ore1，ore3，and ore9 are tolerant to oxidative stress［J］. Plant Cell Physiology，2004，45 (7)：923-932

［5］Cheng H Y，Song S Q Species and organ diversity in the effect of hydrogen peroxide on superoxide dismutase activity in vitro［J］. Journal of Integrative Plant Biology，2006，48 (8)：672-678

［6］张亚冰，刘崇怀，潘兴，等盐胁迫下不同耐盐性葡萄砧木丙二醛和脯氨酸含量的变化［J］. 河南农业科学，2006，35 (4)：84-86 (Zhang Y B，Liu C H，Pan X，et al. Changes of MDA and proline contents in grape rootstocks under NaCl stress［J］. Journal of Henan Agricultural Sciences，2006，35 (4)：84-86)

［7］Hardeland R，Cardinali D P，Srinivasan V，et al. Melatonin-A pleiotropic orchestrating regulator molecule［J］. Progress in Neurobiology，2011，93 (3)：350-384

［8］Lin W，Chao F，Xiao D Z，et al. Plant mitochondria synthesize melatonin and enhance the tolerance of plants to drought stress［J］. Journal of Pineal Research，2017，63(3)：e12429

［9］Zhang N，Sun Q，Zhang H，et al. Roles of melatonin in abiotic stress resistance in plants［J］. Journal of Experimental Botany，2015，66 (3)：647-656

［10］Nawaz M A，Huang Y，Bie Z，et al. Melatonin：Current status and future perspectives in plant science［J］. Frontiers in Plant Science，2016，11 (6)：1230

［11］Tan D X，Tan R，Hardeland L C，et al. Functional roles of melatonin in plants，and perspectives in nutritional and agricultural science［J］. Journal of Experimental Botany，2012，63(2)：577-597

［12］Zhang N，Zhao B，Zhang H J，et al. Melatonin promotes water-stress tolerance，lateral root formation, and seed germination in cucumber (Cucumis sativus L)［J］. Journal of Pineal Research，2013，54 (1)：15-23

［13］Meng J F，Xu T F，Wang Z Z，et al. The ameliorative effects of exogenous melatonin on grape cuttings under water-deficient stress：Antioxidant metabolites，leaf anatomy，and chloroplast morphology［J］. Journal of Pineal Research，2014，57 (2)：200-212

［14］Li H，Chang J，Chen H，et al. Exogenous melatonin confers salt stress tolerance to watermelon by improving photosynthesis and redox homeostasis［J］. Frontiers in Plant Science，2017，8(3)：295

［15］Wei W，Li Q T，Chu Y N，et al. The ameliorative effects of exogenous melatonin on grape cuttings under water-deficient stress：Antioxidant metabolites, leaf anatomy，and chloroplast morphology［J］. Journal of Pineal Research，2014，57 (2)：200-212

［16］Tan W，Liu J，Dai T，et al. Alterations in photosynthesis and antioxidant enzyme activity in winter wheat subjected to post-anthesis water-logging［J］. Photosynthetica，2008，46(1)：21-27

［17］Nakano Y，Asada K Hydrogen peroxide is scavenged by ascorbate peroxidase in spinach chloroplasts［J］. Plant Cell Physiology，1981，22(5)：867-880

［18］李合生植物生理生化试验原理和技术［M］. 北京：高等教育出版社，2000，167-169 (Li H S Principle and technology of plant physiological and biochemical experiments［M］. Beijing：Higher Education Press，2000，167-169)

［19］苍晶，赵会杰植物生理学试验教程［M］. 北京：高等教育出版社，2013，140-163 (Cang J，Zhao H J Experimental course of plant physilology［M］. Beijing：Higher Education Press，2013，140-163)

［20］郭欣欣，李晓锋，朱红芳，等淹水胁迫对不结球白菜抗坏血酸-谷胱甘肽循环的影响［J］. 植物生理学报，2015，51 (12)：2181-2187 (Guo X X，Li X F，Zhu H F，et al. Effects of waterlogging stress on ascorbate-glutathione cycle in Brassica campestrisssp SSP. Chinensis［J］. Plant Physiology Journal，2015，51 (12)：2181-2187)

［21］Liu J，Wang W，Wang L，et al. Exogenous melatonin improves seedling health index and drought tolerance in tomato［J］. Plant Growth Regulation，2015，77 (3)：317-326

［22］Ye J，Wang S W，Deng X P，et al. Melatonin increase dmaize (Zea mays L) seedling drought tolerance by alleviating drought-induced photosynthetic inhibition and oxidative damage［J］. Acta Physiologiae Plantarum，2016，38 (2)：38-48

［23］Xu W Z，Deng X P，Xu B C，et al. Photosynthetic activity and efficiency of Bothriochloa ischaemum and Lespedeza davurica in mixtures across growth periods under water stress［J］. Acta Physiologiae Plantarum，2014，36 (4)：1033-1044

［24］Rivero R M，Mestre T C，Mittler R，et al. The combined effect of salinity and heat reveals a specific physiological，biochemical and molecular response in tomato plants［J］. Plant Cell and Environment，2014，37 (5)：1059-1073

［25］Kocal N，Sonnewald U，Sonnewald S，et al. Cell wall-bound invertase limits sucrose export and is involved in symptom development and inhibition of photosynthesis during compatible interaction between tomato and Xanthomonas campestris pv Vesicatoria［J］. Plant Physiology，2008，148 (3)：1523-1536

［26］Li C，Tan D X，Liang D，et al. Melatonin mediates the regulation of ABA metabolism， free-radical scavenging, and stomatal behaviour in two Malus species under drought stress［J］. Journal of Experimental Botany，2015，66 (3)：669-680

［27］孙哲，范维娟，刘桂玲，等干旱胁迫下外源ABA对甘薯苗期叶片光合特性及相关生理指标的影响［J］. 植物生理学报，2017，53 (5)：873-880 (Sun Z，Fan W J，Liu G L，et al. Effects of exogenous ABA on leaf photosynthetic characteristics and associated physiological indexes of sweetpotato (Ipomoea batatas) seedlings under drought stress［J］. Plant Physiology Journal，2017，53 (5)：873-880)

［28］Turk H，Erdal S，Genisel M，et al. The regulatory effect of melatonin on physiological， biochemical and molecular parameters in cold-stressed wheat seedlings［J］. Plant Growth Regulation，2014，74 (2)：139-152

［29］Maxwell K，Johnson G N Chlorophyll fluorescence-a practical guide［J］. Journal of Experimental Botany， 2000，51 (345)：659-668

［30］Wang P，Sun X，Li C，et al. Long-term exogenous application of melatonin delays drought-induced leaf senescence in apple［J］. Journal of Pineal Research，2013，54 (3)：292-302

［31］Rahdari P，Tavakoli S，Hosseini S M Studying of salinity stress effect on germination， proline，sugar，protein，lipid and chlorophyll content in Purslane (Portulaca oleracea L) leaves［J］. Journal of Stress Physiology and Biochemistry，2012，8 (1)：182-193

［32］刘月，寇从贤，付桂萍，等褪黑素对大豆幼苗盐害的缓解效应及机理研究［J］. 中国油料作物学报，2017，39 (6)：813-819 (Liu Y，Kou C X，Fu G P，et al. Effect of exogenous melatonin on soybean seedlings under salt stress［J］. Chinese Journal of Oil Crop Sciences，2017，39 (6)：813-819)

相似文献/References:

[1]刘章雄,李卫东,孙石,等.1983～2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
　LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(06):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
　LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(06):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
　WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(06):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
　ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(06):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
　LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(06):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
　DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(06):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
　ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(06):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
　GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(06):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
　WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(06):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
　LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(06):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
[11]闫春娟,王文斌,涂晓杰,等.不同生育时期干旱胁迫对大豆根系特性及产量的影响[J].大豆科学,2013,32(01):59.[doi:10.3969/j.issn.1000-9841.2013.01.014]
　YAN Chun-juan,WANG Wen-bin,TU Xiao-jie,et al.Effect of Drought Stress at Different Growth Stage on Yield and Root Characteristics of Soybean[J].Soybean Science,2013,32(06):59.[doi:10.3969/j.issn.1000-9841.2013.01.014]
[12]刘颖,张明怡,韩光,等.干旱胁迫下钾对大豆叶片保护酶活性及产量的影响[J].大豆科学,2011,30(02):341.[doi:10.11861/j.issn.1000-9841.2011.02.0341]
　LIU Ying,ZHANG Ming-yi,HAN Guang,et al.Effect of Potassium on Soybean Leaf Protective Enzymes and Yield under Drought Stress[J].Soybean Science,2011,30(06):341.[doi:10.11861/j.issn.1000-9841.2011.02.0341]
[13]钟鹏,吴俊江,刘丽君,等.干旱胁迫对不同磷效率基因型大豆膜脂过氧化作用的影响[J].大豆科学,2008,27(04):610.[doi:10.11861/j.issn.1000-9841.2008.04.0610]
　ZHONG-Peng,WU Jun-jiang,LIU Li-jun,et al.Effect of Drought Stress on Lipid Peroxidation in Soybean Varieties with Different P Efficiency[J].Soybean Science,2008,27(06):610.[doi:10.11861/j.issn.1000-9841.2008.04.0610]
[14]王伟,姜伟,张金龙,等.大豆种质的耐旱性鉴定及耐旱指标筛选[J].大豆科学,2015,34(05):808.[doi:10.11861/j.issn.1000-9841.2015.05.0808]
　WANG Wei,JIANG Wei,ZHANG Jin-long,et al.Selection of Drought-tolerant Soybean and Evaluation of the Drought.tolerance Indices[J].Soybean Science,2015,34(06):808.[doi:10.11861/j.issn.1000-9841.2015.05.0808]
[15]魏崃,吴广锡,唐晓飞,等.过表达GmHSFA1大豆在干旱条件下对高温的响应[J].大豆科学,2016,35(02):257.[doi:10.11861/j.issn.1000-9841.2016.02.0257]
　WEI Lai,WU Guang-xi,TANG Xiao-fei,et al.Soybean Responses to High Temperatures Under Drought Stress in the Presence of An Over-expressed GmHSFA1 Gene[J].Soybean Science,2016,35(06):257.[doi:10.11861/j.issn.1000-9841.2016.02.0257]
[16]金毅,郑浩宇,金喜军,等.外源ABA、SA及JA对干旱胁迫及复水下大豆生长的影响[J].大豆科学,2016,35(06):958.[doi:10.11861/j.issn.1000-9841.2016.06.0958]
　JIN Yi,ZHENG Hao-yu,JIN Xi-jun,et al.Effect of ABA，SA and JA on Soybean Growth Under Drought Stress and Re-watering[J].Soybean Science,2016,35(06):958.[doi:10.11861/j.issn.1000-9841.2016.06.0958]
[17]刘丽君,尹田夫,孟良.大豆原生质膜及混合细胞器膜脂脂肪酸对干旱胁迫的反应[J].大豆科学,1991,10(01):46.[doi:10.11861/j.issn.1000-9841.1991.01.0046]
　[J].Soybean Science,1991,10(06):46.[doi:10.11861/j.issn.1000-9841.1991.01.0046]
[18]谢甫绨,董钻,赵艺新.大豆器官间的热能分布与耐旱性的关系初报[J].大豆科学,1993,12(02):107.[doi:10.11861/j.issn.1000-9841.1993.02.0107]
　[J].Soybean Science,1993,12(06):107.[doi:10.11861/j.issn.1000-9841.1993.02.0107]
[19]刘莎莎,柏新富,冯春晓,等.干旱条件下土壤盐分对大豆生长及光合作用的影响[J].大豆科学,2017,36(06):921.[doi:10.11861/j.issn.1000-9841.2017.06.0921]
　LIU Sha-sha,BAI Xin-fu,FENG Chun-xiao,et al.Effects of Soil Salinity on the Growth and Photosynthesis of Soybean under Drought Conditions[J].Soybean Science,2017,36(06):921.[doi:10.11861/j.issn.1000-9841.2017.06.0921]
[20]李清,王洪,郭禄芹,等.大豆Whirly基因家族的鉴定和表达分析[J].大豆科学,2019,38(02):204.[doi:10.11861/j.issn.1000-9841.2019.02.0204]
　LI Qing,WANG Hong,GUO Lu-qin,et al.Identification and Expression Analysis of the Whirly Gene Family in Soybean[J].Soybean Science,2019,38(06):204.[doi:10.11861/j.issn.1000-9841.2019.02.0204]

备注/Memo

收稿日期：2018-09-08

基金项目：国家重点研发计划（2018YFD0201000）；国家现代农业产业技术体系(CARS-04-01A)；黑龙江省自然科学基金（C2017049）；黑龙江省农垦总局重点科研计划（HNK135-02-06）。

第一作者简介：邹京南（1989-），男，硕士，主要从事大豆逆境栽培生理研究。E-mail：zoujingnan222@163com。

通讯作者：张玉先（1968-），男，教授，博导，主要从事大豆逆境栽培生理和轮作体系研究。E-mail： zyx_lxy@126com。

胡国华（1951-），男，博士，研究员，主要从事大豆栽培生理和遗传育种研究。E-mail： hugh757@vip163com。

更新日期/Last Update: 2018-12-04