[1]金毅,郑浩宇,金喜军,等.外源ABA、SA及JA对干旱胁迫及复水下大豆生长的影响[J].大豆科学,2016,35(06):958-963.[doi:10.11861/j.issn.1000-9841.2016.06.0958]
　JIN Yi,ZHENG Hao-yu,JIN Xi-jun,et al.Effect of ABA，SA and JA on Soybean Growth Under Drought Stress and Re-watering[J].Soybean Science,2016,35(06):958-963.[doi:10.11861/j.issn.1000-9841.2016.06.0958]
点击复制

外源ABA、SA及JA对干旱胁迫及复水下大豆生长的影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第35卷 期数: 2016年06期 页码: 958-963 栏目: 出版日期: 2016-11-20
Title:
Effect of ABA，SA and JA on Soybean Growth Under Drought Stress and Re-watering
作者:
金毅; 郑浩宇; 金喜军; 张玉先; 任春元
（黑龙江八一农垦大学 农学院，黑龙江 大庆 163319）
Author(s):
JIN Yi;  ZHENG Hao-yu;  JIN Xi-jun;  ZHANG Yu-xian;  REN Chun-yuan
（College of Agronomy, Heilongjiang Bayi Agricultural University, Daqing 163319, China）
关键词:
大豆; 干旱; ABA ; SA; JA; 生长
Keywords:
Soybean;  Drought;  ABA;  SA;  JA;  Growth
DOI:
10.11861/j.issn.1000-9841.2016.06.0958
文献标志码:
A
摘要:
? ? 以干旱敏感型品种黑农65和抗旱品种抗线9号为供试材料，采用沙培方式结合15% PEG-6000模拟干旱胁迫，研究了叶面喷施ABA、SA、JA对干旱及复水下的大豆生长的影响。结果表明：3种外源激素均可以显著降低干旱胁迫及复水下大豆叶片有害物质MDA和过氧化氢含量，同时还能提高干旱胁迫过程中保护性物质可溶性糖和可溶性蛋白含量，大体上表现为ABA的作用效果高于SA和JA，从而降低干旱胁迫对大豆机体的损害程度。3种外源激素均减轻了干旱胁迫对大豆株高、叶、茎、根鲜重和干重的抑制作用，其中JA对干旱敏感型品种黑农65株高、器官鲜、干重的促进效果较好，而SA对抗旱品种抗线9号的促进效果好于ABA和JA。3种外源激素均可降低干旱胁迫导致的大豆落花率，其中SA的作用效果好于ABA和SA。总体来看，外源ABA、SA、JA对干旱胁迫下干旱敏感型品种的促进效果较抗旱品种更明显。
Abstract:
This study was conducted with drought sensitive variety HN65 and drought resistant variety KX9, sand culture and PEG-6000 were used to simulate drought stress to research on the effect of exogenous ABA,JA and SA on soybean growth under drought and re-watering. The result showed that, three exogenous plant hormones all could decrease MDA and hydrogen peroxide content in soybean leaf, which was bad for accumulation of soluble sugar and soluble protein content, protecting soybean from drought and re-water. And ABA showed more significant promotive effect than SA and JA.Three exogenous plant hormones all could alleviate the inhibition of drought on morphological index, JA had more promotive effect than ABA and SA for drought-sensitive soybean variety HN65, and SA had more promotive effect than ABA and JA for drought resistant variety KX9. Three exogenous plant hormones all could decrease drop flower rate of both varieties especially HN65.

参考文献/References:

［1］Araus J L, Slafer G A, Reynolds M P, et al.Plant breeding and drought in C3 cereals: What should we breed for［J］. Annals of Botany, 2002, 89(7):925-940.

［2］高中超，周宝库，张喜林. 大豆对干旱胁迫生理生化的响应［J］. 大豆通报, 2007, 5: 27-30.(Gao Z C, Zhou B K, Zhang X L. The physiology and biochemistry reaction of soybean to drought stress［J］. Soybean Bulletin, 2007, 5: 27-30.)
［3］Bari R, Jones J D G. Role of hormones in plant defense responses［J］. Plant Molecular Biology, 2009, 69: 473-488.
［4］Huang D, Wu W, Abrams S R, et al.The relationship of drought-related gene expression in Arabidopsis thaliana-to hormonal and environmental factors［J］. Journal of Experimental Botany, 2008, 11: 2991-3007.
［5］Lu S Y, Su W, Li H H, et al. Abscisic acid improves drought tolerance of triploid bermudagrass and involves H2O2?and NO- induced antioxidant enzyme activities［J］. Plant Physiology Biochemistry, 2009, 47: 132-138.
［6］Hayat Q, Hayat S, Irfan M, et al. Effect of exogenous salicylic acid under changing environment: A review［J］. Environmental and Experimental Botany, 2010, 8: 14-25.
［7］Horváth E, Szalai G, Janda T.Induction of abiotic stress tolerance by salicylic acid signaling［J］ Journal of Plant Growth Regulation, 2007, 26: 290-300.?
［8］Kadioglu A, Saruhan N, Saglam A, et al. Exogenous salicylic acid alleviates effects of long term drought stress and delays leaf rolling by inducing antioxidant system［J］. Plant Growth Regulation, 2010, 64: 27-37.?
［9］Ai L, Li Z H, Xie Z X, et al. Coronatine alleviates polyethylene glycol-induced water stress in two rice (Oryza sativa L) cultivars［J］. Journal?
?［10］Pedranzani H, Racagni G, Alemano S, et al. Salt tolerant tomato plants show increased levels of jasmonic acid［J］. Plant Growth Regulation, 2003, 41: 149-158.
［11］Wu C Y, Anthony T, Parthiban R, et al. Brassinosteroids regulate grain filling in rice［J］. The Plant Cell, 2008, l20: 2130-2145.
［12］Rita S C, Vrinda S T. Relationship between gibberellic acid and growth parameters in developing seed and pod of pigeon pea［J］. The Brazilian Journal of Plant Physiology, 2007, 19(1): 43-51.
［13］Ibrahim M H, Jaafar H Z. Primary, secondary metabolites, H2O2, malondialdehyde and photosynthetic responses of orthosiphon stimaneus benth. To different irradiance levels［J］ Molecules, 2012, 17, 1159-1176.
.［14］周博如, 李永镐, 刘太国, 等. 不同抗性的大豆品种接种大豆细菌性疫病菌后可溶性蛋白、总糖含量变化的研究［J］. 大豆科学, 2000, 19(2): 111-114. (Zhou B R, Li Y G, Liu T G.Studies on the changes of soluble protein and total sugar in the first leaves of soybean varieties inoculated with Pseudomonas syringes pv glycinea［J］.Soybean Science, 2000, 19(2):111-114.)
［15］路阳. 用考马斯亮蓝G-250迅速地测定蛋白质浓度［J］.生物学杂志, 1992(1): 24-25 (Lu Y. Quick measure of protein concentration with coomassie brilliant blue G.250［J］. Journal of Biology, 1992(1): 24-25.)
［16］王敏, 张从宇, 马同富, 等. 大豆品种苗期抗旱性研究［J］.中国油料作物学报, 2004, 26(3): 29-32. (Wang M, Ma C Y, Ma T F, et al.Studies on the drought resistance of seedling in soybean［J］. Chinese Journal of Oil Crop Scieves, 2004, 26(3): 29-32.)
［17］Farooq M, Wahid A, Lee D J, et al. Comparative time course action of the foliar applied glycinebetaine, salicylic acid, nitrous oxide, brassinosteroids and spermine in improving drought resistance of rice［J］. Journal of Agronomy and Crop Science, 2010, 196: 336-345.
［18］Noctor G, Veljovic-Jovanovic S D, Driscoll S, et al. Drought and oxidative load in wheat leaves.A predominant role for photorespiration ［J］. Annals of Botany, 2002, 89, 841-850.
［19］赵江涛, 李晓峰, 李航, 等. 可溶性糖在高等植物代谢调节中的生理作用［J］. 安徽农业科学, 2006, 34(24): 6423-6425. (Zhao J T, Li X F, Li H, et al. The physiological function of soluble sugar metabolic regulation in higher plants［J］. Journal of Anhui Agricultural Sciences, 2006, 34(24): 6423-6425.)

相似文献/References:

[1]刘章雄,李卫东,孙石,等.1983～2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
　LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(06):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
　LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(06):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
　WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(06):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
　ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(06):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
　LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(06):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
　DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(06):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
　ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(06):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
　GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(06):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
　WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(06):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
　LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(06):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
[11]闫春娟,王文斌,涂晓杰,等.不同生育时期干旱胁迫对大豆根系特性及产量的影响[J].大豆科学,2013,32(01):59.[doi:10.3969/j.issn.1000-9841.2013.01.014]
　YAN Chun-juan,WANG Wen-bin,TU Xiao-jie,et al.Effect of Drought Stress at Different Growth Stage on Yield and Root Characteristics of Soybean[J].Soybean Science,2013,32(06):59.[doi:10.3969/j.issn.1000-9841.2013.01.014]
[12]刘颖,张明怡,韩光,等.干旱胁迫下钾对大豆叶片保护酶活性及产量的影响[J].大豆科学,2011,30(02):341.[doi:10.11861/j.issn.1000-9841.2011.02.0341]
　LIU Ying,ZHANG Ming-yi,HAN Guang,et al.Effect of Potassium on Soybean Leaf Protective Enzymes and Yield under Drought Stress[J].Soybean Science,2011,30(06):341.[doi:10.11861/j.issn.1000-9841.2011.02.0341]
[13]钟鹏,吴俊江,刘丽君,等.干旱胁迫对不同磷效率基因型大豆膜脂过氧化作用的影响[J].大豆科学,2008,27(04):610.[doi:10.11861/j.issn.1000-9841.2008.04.0610]
　ZHONG-Peng,WU Jun-jiang,LIU Li-jun,et al.Effect of Drought Stress on Lipid Peroxidation in Soybean Varieties with Different P Efficiency[J].Soybean Science,2008,27(06):610.[doi:10.11861/j.issn.1000-9841.2008.04.0610]
[14]王伟,姜伟,张金龙,等.大豆种质的耐旱性鉴定及耐旱指标筛选[J].大豆科学,2015,34(05):808.[doi:10.11861/j.issn.1000-9841.2015.05.0808]
　WANG Wei,JIANG Wei,ZHANG Jin-long,et al.Selection of Drought-tolerant Soybean and Evaluation of the Drought.tolerance Indices[J].Soybean Science,2015,34(06):808.[doi:10.11861/j.issn.1000-9841.2015.05.0808]
[15]魏崃,吴广锡,唐晓飞,等.过表达GmHSFA1大豆在干旱条件下对高温的响应[J].大豆科学,2016,35(02):257.[doi:10.11861/j.issn.1000-9841.2016.02.0257]
　WEI Lai,WU Guang-xi,TANG Xiao-fei,et al.Soybean Responses to High Temperatures Under Drought Stress in the Presence of An Over-expressed GmHSFA1 Gene[J].Soybean Science,2016,35(06):257.[doi:10.11861/j.issn.1000-9841.2016.02.0257]
[16]刘丽君,尹田夫,孟良.大豆原生质膜及混合细胞器膜脂脂肪酸对干旱胁迫的反应[J].大豆科学,1991,10(01):46.[doi:10.11861/j.issn.1000-9841.1991.01.0046]
　[J].Soybean Science,1991,10(06):46.[doi:10.11861/j.issn.1000-9841.1991.01.0046]
[17]谢甫绨,董钻,赵艺新.大豆器官间的热能分布与耐旱性的关系初报[J].大豆科学,1993,12(02):107.[doi:10.11861/j.issn.1000-9841.1993.02.0107]
　[J].Soybean Science,1993,12(06):107.[doi:10.11861/j.issn.1000-9841.1993.02.0107]
[18]刘莎莎,柏新富,冯春晓,等.干旱条件下土壤盐分对大豆生长及光合作用的影响[J].大豆科学,2017,36(06):921.[doi:10.11861/j.issn.1000-9841.2017.06.0921]
　LIU Sha-sha,BAI Xin-fu,FENG Chun-xiao,et al.Effects of Soil Salinity on the Growth and Photosynthesis of Soybean under Drought Conditions[J].Soybean Science,2017,36(06):921.[doi:10.11861/j.issn.1000-9841.2017.06.0921]
[19]邹京南,金喜军,王孟雪,等.外源褪黑素对干旱胁迫条件下大豆苗期光合及生理的影响[J].大豆科学,2018,37(06):896.[doi:1011861/jissn1000-98412018060896]
　ZOU Jing-nan,JIN Xi-jun,WANG Meng-xue,et al.Effects of Exogenous Melatonin on Photosynthesis and Physiology of Soybean Seedlings under Drought Stress[J].Soybean Science,2018,37(06):896.[doi:1011861/jissn1000-98412018060896]
[20]李清,王洪,郭禄芹,等.大豆Whirly基因家族的鉴定和表达分析[J].大豆科学,2019,38(02):204.[doi:10.11861/j.issn.1000-9841.2019.02.0204]
　LI Qing,WANG Hong,GUO Lu-qin,et al.Identification and Expression Analysis of the Whirly Gene Family in Soybean[J].Soybean Science,2019,38(06):204.[doi:10.11861/j.issn.1000-9841.2019.02.0204]

备注/Memo

基金项目：国家级大学生创新项目（201510223006）；国家自然科学基金（31401332，204134008）；国家“十二五”科技支撑计划（2014BAD11B01-02）；国家现代农业产业技术体系建设专项（CARS-04-PS17）。

第一作者简介：金毅（1995-），男，学士，主要从事大豆栽培与生理研究。E-mail：18345966737@163.com。
通讯作者：张玉先（1968-），男，教授，主要从事大豆栽培研究。E-mail：zyx_lxy@126.com。

更新日期/Last Update: 2016-12-09