[1]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19-27.[doi:10.3969/j.issn.1000-9841.2013.01.005]
　ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(01):19-27.[doi:10.3969/j.issn.1000-9841.2013.01.005]
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线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第32卷 期数: 2013年01期 页码: 19-27 栏目: 出版日期: 2013-02-25
Title:
Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers
作者:
张春宝1; 李玉秋1; 彭宝1; 王鹏年2; 董英山1; 赵丽梅1
1.吉林省农业科学院 大豆研究所，吉林 长春 130033；
2.吉林农业大学 农学院，吉林 长春 130022
Author(s):
ZHANG Chun-bao1;  LI Yu-qiu1;  PENG Bao1;  WANG Peng-nian2;  DONG Ying-shan1;  ZHAO Li-mei1
1.Soybean Research Institute,Jilin Academy of Agricultural Sciences,Changchun 130033；
2.College of Agronomy,Jilin Agricultural University,Changchun 130022,Jilin,China
关键词:
大豆; 不育系; 保持系; 杂交种; 分子标记
Keywords:
Soybean; Sterile line; Maintainer line; Hybrid; Molecular markers
分类号:
S565.1
DOI:
10.3969/j.issn.1000-9841.2013.01.005
摘要:
通过提取细胞质雄性不育系和同型保持系的线粒体基因组DNA,用100条ISSR引物进行筛选,发现引物ISSR829的扩增片段在不育系和保持系间存在差异。对差异片段进行测序发现,该序列仅存在于保持系线粒体基因组中,且位于基因Atp6上游2 000 bp处,推断其可能与不育有关。利用测序获得片段设计引物,用目前已育成的5个大豆杂交种的不育系母本和同型保持系的基因组DNA为模版,进行PCR扩增,发现不育系母本未出现特异片段,而对应保持系出现目的片段,表明该特异片段转化为SCAR标记,可用于不育系的筛选和不育系种子纯度鉴定。
Abstract:
By extracting the mitochondrial DNA of cytoplasmic male sterile line and its maintainer line,screening with 100 ISSR primers,and it was found that the amplification product of primer ISSR829 had differences in sterile line and its maintainer.Then sequenced the different fragments and found that the sequence was only exist in the maintainer mitochondrial genome,and upstream of mitochondrial gene Atp6 by 2 000 bp.It was suggested that the sequence has a relationship with infertility.Designed primers according to the sequence and detected female parent and its maintainer line of five soybean hybrids,and found that sterile line did not appear specific fragment,but a target fragment corresponding maintainer lines.It was confirmed that the specific fragments was converted to a SCAR marker,which could be used for testing the seed purity of cytoplasmic male sterile line.

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备注/Memo

基金项目：国家自然科学基金(31201224)；国家高技术研究发展计划“863计划”重点项目(2011AA10A105)；中国博士后基金 (2012M510902)；吉林省青年科研基金 (201201091)；吉林省优秀创新团队项目（20111817）。

第一作者简介：张春宝(1980-)，男，博士，副研究员，研究方向为大豆杂种优势利用。E-mail:cbzhang@126 com。
通讯作者：:赵丽梅(1964-)，女，博士，研究员，研究方向为大豆杂种优势利用。E-mail:l_mzhao@126.com。

更新日期/Last Update: 2013-02-20