[1]刘尚前,王罡,季静,等.农杆菌介导法将Bt基因转入大豆的研究[J].大豆科学,2007,26(01):103-106.[doi:10.3969/j.issn.1000-9841.2007.01.024]
　LIU Shang-qian,WANG Gang,JI Jing,et al.PUT Bt GENE TRANSFER INTO SOMATIC VIA AGROBACTERIUM TUMEFACIEN[J].Soybean Science,2007,26(01):103-106.[doi:10.3969/j.issn.1000-9841.2007.01.024]
点击复制

农杆菌介导法将Bt基因转入大豆的研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第26卷 期数: 2007年01期 页码: 103-106 栏目: 出版日期: 2007-02-25
Title:
PUT Bt GENE TRANSFER INTO SOMATIC VIA AGROBACTERIUM TUMEFACIEN
文章编号:
1000-9841(2007)01-0103-04
作者:
刘尚前1; 王罡2; 季静2; 王萍3
1.河北北方学院农业科学系，宣化075131；
2.天津大学农业与生物工程学院，天津300072；
3淮海工学院海洋学院，连云港222005
Author(s):
LIU Shang-qian1; WANG Gang2; JI Jing2; WANG Ping3
1.Department of Agriculture Sciences of Hebei North University， Xuanhua 075131;
2.Tianjin University of Agricultural and Biological Engineering, Tianjin 300072;
3.Huaihai Institute of Technology， Marine School，Lianyungang 222005
关键词:
大豆; 体细胞胚; Bt基因; 遗传转化
Keywords:
Soybean;  Somatic embryos;  Bt gene;  Genetic transformation
分类号:
S565.103.53
DOI:
10.3969/j.issn.1000-9841.2007.01.024
文献标志码:
A
摘要:
以6个品种大豆体细胞胚为受体，以Bt基因为目的基因，应用根癌农杆菌介导法对大豆进行了遗传转化，经体细胞胚萌发、生根及壮苗培养和卡那霉素选择培养，获得了完整的抗性再生苗，经PCR、PCR-Southern和点杂交分析鉴定，初步证明外源基因Bt已整合到大豆的基因组中。
Abstract:
Somatic embryos of 6 different soybean were transformed by Agrobacterium tumefaciens. The resistant plants to kanamycin were obtained. 5 positive plants were obtained among them by PCR. These positive plantlets were further analyzed by dot blotting and PCRSouthern blotting. The results demonstrated that the target gene had been transformed into the soybean genome.

参考文献/References:

［1］Hinchee Maw，DVConner Ward，Ca Newell，et al.Production of transgenic soybean plants using agrobacterium -mediated DNA transfer ［J］.bio/technol.1988，6(91)：2-5.

［2］Mccabe DE，WF Swain，BJ Martinell, et al. Stable transformation of soybean (Glycine max)by particle acceleration［J］.bio/technol.1988，6(92)： 3-6.
［3］王关林，方宏筠.植物基因工程原理与技术［M］.北京：科学出版社，2002：386-389.
［4］王萍，吴颖，何娜,等.抗生素对农杆菌抑制的效果和大豆外植体诱导的影响［J］.东北师大学报自然科学版.遗传学研究学专辑，2000，11：39-41.
［5］王萍，吴颖，季静,等.抗生素对大豆愈伤组织的诱导和生长的影响［J］. 遗传，2001，23（4）：321-324.
［6］王罡，季静，王萍,等.大豆基因型对根癌农杆菌菌株敏感性的研究［J］.遗传，2002，24（2）：297-300.
［7］贾士荣.T-DNA转移机理［J］.植物生理学通讯，1994，30(4)：306-311.

相似文献/References:

[1]刘章雄,李卫东,孙石,等.1983～2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
　LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
　LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
　WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
　ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
　LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
　DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
　ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
　GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
　LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
[10]杨如萍,张国宏,王立明,等.甘肃省大豆主产区产量性状及品质分析[J].大豆科学,2013,32(01):50.[doi:10.3969/j.issn.1000-9841.2013.01.012]
　YANG Ru-ping,ZHANG Guo-hong,WANG Li-ming,et al.Analysis on Yield and Quality Traits of Soybean in Different Planting Region of Gansu[J].Soybean Science,2013,32(01):50.[doi:10.3969/j.issn.1000-9841.2013.01.012]
[11]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
　WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[12]王 萍,李 倩,王 罡,等.基因枪法转化大豆未成熟子叶影响因素的研究[J].大豆科学,2013,32(06):735.[doi:10.11861/j.issn.1000-9841.2013.06.0735]
[13]王萍,钟影,王罡,等.大豆体细胞胚的诱导及对草甘膦耐性的研究[J].大豆科学,2012,31(01):152.[doi:10.3969/j.issn.1000-9841.2012.01.035]
　WANG Ping,ZHONG Ying,WANG Gang,et al.Induction of Soybean Somatic Embryos and Their Tolerance to Glyphosate[J].Soybean Science,2012,31(01):152.[doi:10.3969/j.issn.1000-9841.2012.01.035]
[14]王晓春,王罡,季静,等.2,4-D对大豆体细胞胚胎发生及增殖的影响[J].大豆科学,2009,28(06):1113.[doi:10.11861/j.issn.1000-9841.2009.06.1113]
　WANG Xiao-chun,WANG Gang,JI Jing,et al.Somatic Embryogenesis and Proliferation of Soybean Embryogenic Tissue Treated with 2，4-D[J].Soybean Science,2009,28(01):1113.[doi:10.11861/j.issn.1000-9841.2009.06.1113]
[15]王晓春,王罡,季静,等.用基因枪法将Bt基因转入大豆的研究[J].大豆科学,2007,26(02):140.[doi:10.3969/j.issn.1000-9841.2007.02.004]
　WANG Xiao-chun,WANG Gang,JI Jing,et al.PUT BT GENE TRANS FORMATION INTO SMATIC VIA MICROPROJECTILE BOMBARDMENT[J].Soybean Science,2007,26(01):140.[doi:10.3969/j.issn.1000-9841.2007.02.004]
[16]王萍,郭永来,高世庆,等.基因枪法将GmDREB基因导入大豆的研究[J].大豆科学,2007,26(03):315.[doi:10.3969/j.issn.1000-9841.2007.03.005]
　WANG Ping,GUO Yong-lai,GAO Shi-qing,et al.TRANSFORMING GmDREB GENE INTO SOYBEAN VIA PARTICLE BOMBARDMENT[J].Soybean Science,2007,26(01):315.[doi:10.3969/j.issn.1000-9841.2007.03.005]
[17]张小明,薛永国.大豆突变体库构建研究进展[J].大豆科学,2016,35(02):343.[doi:10.11861/j.issn.1000-9841.2016.02.0345]
　ZHANG Xiao-ming,XUE Yong-guo.Research Progress of Soybean Mutant Library Construction[J].Soybean Science,2016,35(01):343.[doi:10.11861/j.issn.1000-9841.2016.02.0345]
[18]王晓春.农杆菌介导的大豆体细胞胚遗传转化影响因子的研究*[J].大豆科学,2005,24(01):21.[doi:10.11861/j.issn.1000-9841.2005.01.0021]
　Wang XiaochunWang GangJi JingWang PingLiu Shangqian.THE FACTORS INFLUENCING GENETIC TRANSFORMATION SYSTEM INSOMATIC EMBRYOS OF SOYBEAN MEDIATED BY AGROB ACTERIUM[J].Soybean Science,2005,24(01):21.[doi:10.11861/j.issn.1000-9841.2005.01.0021]
[19]王晓春刘尚前季静王萍.影响大豆体细胞胚萌发率的因素[J].大豆科学,2004,23(02):151.[doi:10.11861/j.issn.1000-9841.2004.02.0151]
　Wang XiaochunLiu ShangqianJi Jing Wang Ping.THE AFFECT FACTORS ON THE GERMINATION FREQUENCYOFSOMATIC EMBRYOS OF SOYBEAN[J].Soybean Science,2004,23(01):151.[doi:10.11861/j.issn.1000-9841.2004.02.0151]
[20]王晓春,等.农杆菌介导的大豆体细胞胚遗传转化系统的优化研究[J].大豆科学,2004,23(03):200.[doi:10.11861/j.issn.1000-9841.2004.03.0200]
　Wang Xiaochun,Liu ShangqianJi Jing,et al.OPTIMIZATION OF GENETIC TRANSFOMATION SYSTEM IN SOMATIC EMBRYOS OFSOYBEAN MEDIATED BY AGROB ACTER IUM TUMEFACIENS[J].Soybean Science,2004,23(01):200.[doi:10.11861/j.issn.1000-9841.2004.03.0200]

备注/Memo

基金项目：本研究由“国家植物转基因技术研究开发与中试基地建设”专项课题（J99-B-001）资助

作者简介：刘尚前（1972-），男，讲师，硕士，主要从事作物栽培与遗传育种工作研究。

更新日期/Last Update: 2014-10-21