Wang Xiaochun,Liu ShangqianJi Jing,et al.OPTIMIZATION OF GENETIC TRANSFOMATION SYSTEM IN SOMATIC EMBRYOS OFSOYBEAN MEDIATED BY AGROB ACTER IUM TUMEFACIENS[J].Soybean Science,2004,23(03):200-204.[doi:10.11861/j.issn.1000-9841.2004.03.0200]
农杆菌介导的大豆体细胞胚遗传转化系统的优化研究
- Title:
- OPTIMIZATION OF GENETIC TRANSFOMATION SYSTEM IN SOMATIC EMBRYOS OFSOYBEAN MEDIATED BY AGROB ACTER IUM TUMEFACIENS
- 文章编号:
- 1000 -9841(2004)03 -0200 -05
- Keywords:
- Soybean ; Somatic embryos ; Genetic transformation
- 分类号:
- S 565.103.53
- 文献标志码:
- A
- 摘要:
- 以大豆球形期体细胞胚为外植体, 以抗性体细胞胚筛选率为指标, 对影响农杆菌介导的大豆体细胞胚遗传转化频率的因子进行了优化。结果表明, 菌液浓度以OD600 =0 .5 -0 .7 、AS 浓度为50 -100μmol/L 、侵染时间10 分钟、共培养时间2 -3 天, 有利于提高转化率。
- Abstract:
- The factors influencing genetic transformation about somatic embryos of soy bean had been studied .Mediated by Agrobacterium tumefaciens .The sui table condi tio n w as determined as followed :injured treatmentof somatic embryos of soybean , the bacterial concent ration reached 0 .5 -0 .7 (OD600) , 10 minutes infect iontime , 50 -100μmol/L AS .The t ransformation f requency increased in those condi tio n .
参考文献/References:
1 Hinchee Maw , DVConner -Ward , Ca New ell , et al.Production oftransgenic soybean Plants using Ag robact erium -mediat ed DNAtransf er[ J] .BIO/ T ECHNOL .1988 , 6(91):2 -5
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备注/Memo
基金项目:本研究由“ 国家植物转基因技术研究开发与中试基地建设” 专项课题(J99 -B -001)资助