[1]王晓春,王罡,季静,等.2,4-D对大豆体细胞胚胎发生及增殖的影响[J].大豆科学,2009,28(06):1113-1114,1122.[doi:10.11861/j.issn.1000-9841.2009.06.1113]
　WANG Xiao-chun,WANG Gang,JI Jing,et al.Somatic Embryogenesis and Proliferation of Soybean Embryogenic Tissue Treated with 2，4-D[J].Soybean Science,2009,28(06):1113-1114,1122.[doi:10.11861/j.issn.1000-9841.2009.06.1113]
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2,4-D对大豆体细胞胚胎发生及增殖的影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第28卷 期数: 2009年06期 页码: 1113-1114,1122 栏目: 出版日期: 2009-12-25
Title:
Somatic Embryogenesis and Proliferation of Soybean Embryogenic Tissue Treated with 2，4-D
文章编号:
1000-9841(2009)06-1112-03
作者:
王晓春1 ; 王罡2 ; 季静2; 王萍3
1.河北北方学院 农林科技学院 农业科学系，河北 宣化 075131；
2.天津大学 农业与生物工程学院，天津 300072；
3.淮海工学院 海洋学院，江苏 连云港 222005
Author(s):
WANG Xiao-chun1 ; WANG Gang2; JI Jing2; WANG Ping3
1.Department of Agriculture Sciences of Hebei North University，Xuanhua 075131，Hebei；
2.Tianjin University of Agricultural and Biological Engineering，Tianjin 300072；
3.Huaihai Institute of Technology，Marine School，Lianyungang 222005，Jiangsu，China
关键词:
大豆; 基因型; 体细胞胚; 继代增殖
Keywords:
2; 4-D concentrations; Soybean; Somatic embryos; Proliferation
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2009.06.1113
文献标志码:
A
摘要:
研究不同浓度的2，4-D对不同基因型大豆体细胞胚胎发生及增殖的影响。结果表明：不同基因型大豆体细胞胚胎发生率存在显著差异，在供试的6种基因型中，以CH21141和黑农40体细胞胚胎发生率最高，黑农35诱导率最低；大豆体细胞胚胎发生及继代增殖的最佳2，4-D浓度为10~20 mg.L -1，继代扩繁系数为3n(n为继代培养次数)。
Abstract:
Taking immature cotyledon of six soybean varieties as explants，the effect of 2，4-D on the frequencies of somatic embryogenesis and the proliferation of embryogenic tissue were investigated.Results showed that the frequencies of somatic embryogenesis varied with soybean genotypes，CH21141 and Heinong 40 had highest frequencies ，while Heinong 35 had the lowest frequency among six genotypes.The optimum media for somatic embryogenesis and the proliferation of embryogenic tissue was MS with 10-20 mg.L-12，4-D，and the proliferation speed of progeny was 3n.

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备注/Memo

基金项目：国家植物转基因技术研究开发与中试基地建设专项课题资助项目(J99-B-001)。

第一作者简介：王晓春（1971-），女，副教授，硕士，现从事作物栽培与遗传育种工作与研究。E-mail:wangxiaochun305@sina.com。

更新日期/Last Update: 2014-09-13