[1]王萍,郭永来,高世庆,等.基因枪法将GmDREB基因导入大豆的研究[J].大豆科学,2007,26(03):315-318.[doi:10.3969/j.issn.1000-9841.2007.03.005]
　WANG Ping,GUO Yong-lai,GAO Shi-qing,et al.TRANSFORMING GmDREB GENE INTO SOYBEAN VIA PARTICLE BOMBARDMENT[J].Soybean Science,2007,26(03):315-318.[doi:10.3969/j.issn.1000-9841.2007.03.005]
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基因枪法将GmDREB基因导入大豆的研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第26卷 期数: 2007年03期 页码: 315-318 栏目: 出版日期: 2007-06-25
Title:
TRANSFORMING GmDREB GENE INTO SOYBEAN VIA PARTICLE BOMBARDMENT
文章编号:
1000-9841(2007)03-0315-04
作者:
王萍1; 郭永来2 ; 高世庆3; 马有志3; 程宪国4 ; 李文滨5
1淮海工学院海洋学院，连云港222005；
2长春龙洋大豆生物蛋白股份有限公司，长春 130041；
3中国农业科学院作物栽培育种研究所，北京 100081；
4中国农业科学院土肥所，北京 100081；
5东北农业大学大豆研究所/国家教育部大豆生物学重点实验室，哈尔滨 150030
Author(s):
WANG Ping1; GUO Yong-lai2 ; GAO Shi-qing3; MA You-zhi3; CHENG Xian-guo4; LI Wen-bin5
3Institute of Crop Breeding and Cultivation,Chinese Academy of Agricultural Sciences,Beijing 100081;4Institute of Soil and Fertility,Chinese Academy of Agricultural Sciences,Beijing 100081;5Soybaen Research Institute Key Laboratory of Soybean Biology in Chinese Ministry of Education Northeast Agricultural University,Harbin 150030
关键词:
大豆; 基因枪; GmDREB基因; 体细胞胚
Keywords:
Soybean; Particle bombardment; GmDREB gene; Somatic embryo
分类号:
S565.103.53
DOI:
10.3969/j.issn.1000-9841.2007.03.005
文献标志码:
A
摘要:
测定大豆体细胞胚对PPT的基础抗性，用基因枪法将pRdGM-bar质粒转化大豆体细胞胚，得到抗性体细胞胚和抗性植株，经PCR、PCRsouthern检测，证明GmDREB基因转入到大豆中。
Abstract:
The basis resistance of somatic embryo in soybean to PPT was tested.Plasmid pRdGM-bar was transformed into somatic embryo of soybean via particle bombardment.The resistant somatic embryos and plantlets were obtained.It was demonstrated that the GmDREB gene had been transformed into soybean by polymerase chain reaction and PCR-Southern detection

参考文献/References:

［1］McCabe Dennis E,Swain William F,Martinell Brian J,et al.Stable transformation of soybean(Glycine max)by particle acceleration[J]Bio/Technology,1988,6:923-926.

［2］Maughan PJ,Philip R,Cho M-J,et al.Biolistic transformation,expression and inheritance of bovine β-casein in soybean (Glycine max)[J]In Vitro Cell Biol,1999,35P:344-349.
［3］Wang Ping,Wang Gang,Ji Jing,et al.A novel system for proliferation,maintenance and plantlet germination from somatic embryo of soybean[J].Acta Botanica Sinica,2004,46(2):154-158.
［4］龚学臣，季静，王罡，等.基因枪对大豆进行PSY基因的转化研究[J].大豆科学，2006，25（2）：137-140.
［5］王关林，方宏筠主编.植物基因工程原理与技术[M].北京：科学出版社，1998，598-625.

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备注/Memo

基金项目：教育部大豆生物学重点实验室资助项目（SB06A07）;国家植物转基因研究与产业化专项（JY03-A-18）

作者简介：王萍（1957-），女，教授，博士，主要从事生物技术与植物转基因的研究。E-mail:y_pwang@yahoo.com.cn

更新日期/Last Update: 2014-10-22