[1]罗庆云　於丙军　刘友良.大豆苗期耐盐性鉴定指标的检验[J].大豆科学,2001,20(03):177-182.[doi:10.11861/j.issn.1000-9841.2001.03.0177]
　Luo Qingyun Yu Bingjun Liu Youliang.EFFECTOF NaCl ON THE GROWTH,K+ ,Na+ AND Cl -DISTRIBUTION INSEEDLINGS OF 6 SOYBEAN CULTIVARS(Glycine max L.Merril l)[J].Soybean Science,2001,20(03):177-182.[doi:10.11861/j.issn.1000-9841.2001.03.0177]
点击复制

大豆苗期耐盐性鉴定指标的检验

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第20卷 期数: 2001年03期 页码: 177-182 栏目: 出版日期: 2001-08-25
Title:
EFFECTOF NaCl ON THE GROWTH,K+ ,Na+ AND Cl -DISTRIBUTION INSEEDLINGS OF 6 SOYBEAN CULTIVARS(Glycine max L.Merril l)
文章编号:
1000 -9841(2001)03 -0177 -06
作者:
罗庆云　於丙军　刘友良
南京农业大学农业部作物生长调控重点实验室, 南京210095
Author(s):
Luo Qingyun 　Yu Bingjun 　Liu Youliang
Key Lab of Crop Growth Regulation , Minist ry of Agriculture , NanjingAgricultural University , Nanjing 210095
关键词:
大豆; 耐盐性; 耐盐性鉴定; K+; Na+; K+/Na+; Cl-
Keywords:
Soybean ; Salt tolerance ; Evalution of salt tolerance ; K+ ; Na+; Ratio of K+to Na+ ; Cl-
分类号:
S332.1
DOI:
10.11861/j.issn.1000-9841.2001.03.0177
文献标志码:
A
摘要:
在大豆2 叶1 心期, 以含NaCl 浓度分别为0 、50 、100 和150mmol·L- 。1 的1/2Hoag land 营养液进行培养。14 天后, 以植株衰亡叶面积率结合正常植株率为指标鉴定了6 个栽培大豆品种的苗期耐盐性, 同时考查NaCl 胁迫对大豆苗期生长状况和发育进程以及大豆体内K+ 、Na+和Cl-分配情况的影响。结果表明, Lee68 和南农1138 -2 的耐盐性较强;南农88 -31 为中度耐盐大豆品种;苏协1 号和Jackson 的耐盐性较差;中子黄豆乙的耐盐性最弱。NaCl 抑制植株地上部干物质的积累, 使植株的茎节数减少和株高变小, 提高根冠比;在NaCl 胁迫下, 耐盐性强的大豆品种的叶部对K+的选择性吸收和对Na+ 、Cl-的排斥性以及根部对Na+和Cl-的截留作用强。
Abstract:
Seedlings w ith two fully expanded leaves w ere cultivated in 1/2 ’ hoagland’ nut rient solution containing0 , 50 , 100 , 150mmol·L-1 NaCl for 14 day s .Then average percentage of senescenced leaf area and percentageof no rmal seedling were employed as indexes in salt tolerance of 6 soybean cultivars (Glycine max L .Merrill).The evalution of NaCl effect s on the grow th , K+ ,Na+and Cl-dist ribution in seedlings w ere surveyed .The results show ed that , according to their ability of salt tolerance , ”Lee68” and ”Nannong 1138 -2” were ranged as’ strong’ , ”Nannong 88 -31” w as ranged as ’ moderate’ , ” No .1 of Suxie” and ” Jackson” were ranged as ’w eak’ and ”Jiangsu Zhongzi huangdou -yi” was the ’ w eakest’ o ne .Accumulat ion of shoot biomass, height andstem node No .of seedling were reduced dramatically .Ratio of roo ts to shoot s w as increased .Compared to saltsensitive varieties , the leaf of salt tolerance ’ st rong’ varieties w as characterized as st rong selector of K+ , andtheir shoot s and root s were characterized as st rong impediments in upw ard t ransport of Na+and Cl-.

参考文献/References:

1 　S tumpf , D .K., Prisco .J .T ., Weeks , J .R., et al., Salinity andS alicorni a bigelovii Torr.Seedling establishment[ J] .Wat er relations.J .Exp .Bot.1986(37):160 -169.

2 　Wahid , A ., Rao , A.R., Rasul , E .Germination of seeds andpropagules under salt stress[ S] .In :Peessarakli , M .(Ed.), Hand book of Plant and Corp S t ress.2 , Marcel Dekker , New York ,1999 , pp.153 -167.
3 　Wahid , A ., Rao , A .R., Rasul , E .Identif ication of salt tolerancet rait s in sugarcane lines[ J] .Field Crops Res ., 1997(54):9 -17.
4 　Lut t s, S ., Kinet , J .M .Bouharmont , J .NaCl induced senescencein leaves of rice(Oryza sativa L .)cult ivars dif fering in salinity resistance[J] .Ann .Bot .1996(78):389 -398.
5 　Garcia , A ., Senadhira, Flowers , T .J ., et al., The eff ect of selectionfor sodium transport and for agronomic charact eristi c upon sal tresist ance in ri ce(Oryza sativa L .)[ J] .Theor .Appl.Genet , 1995(90):1106 -1111.
6 　Francois , L.E., Salini ty ef fects on four sunf low er hybrids[ J] .Agron.J .1996(88):215 -219.
7 　龚明, 刘友良, 丁念诚, 等.大麦不同生育期的耐盐性差异[ J] .西北植物学报, 1994 , 14(1):1 -7.
8 　常汝镇, 陈一舞, 邵桂花, 等.盐对大豆农艺性状及籽粒品质的影响[ J] .大豆科学, 1994 , 13(2):101 -105.
9 　邵桂花, 常汝镇, 陈一舞, 等.大豆耐盐性研究进展[ J] .大豆科学, 1993 , 12(3):244 -248.
10 　马淑时, 王伟.大豆品种资源的抗盐碱性研究[ J] .吉林农业科学, 1994 ,4 :69 -71.
11 　H U , ZA., Wang , HX.Salt t olerance of wild soybean (Glycinesoja )in natu ral populations evalu ted by a new method[ J] .SoybeanGenetics New slet ter , 1997 , 24 :79 -80.
12 　郭蓓, 邱丽娟, 邵桂花, 等.大豆耐盐基因的PC R 标记[ J] .中国农业科学, 2000 , 33(1):10 -16.
13 　George H .Abel , Arnold J .Mackenzie.Salt tolerance of soybeanvarieties (Glycine max L.Merrill)during germination and latergrow th[ J] .Corp Science, 1966 , 4 , 157 -160.
14 　毛达如主编.植株营养研究方法[ M] .北京, 北京农业大学出版社, 1994 , 17.
15 　王宝山, 赵可夫.小麦叶片中Na+ 、K +提取方法的比较[ J] .植物生理学通讯, 1995 , 31(1):50 -52.
16 　刘祖祺, 张石城主编.植物抗性生理学[ M] .北京, 中国农业出版社, 1993 , 278.
17 　徐云岭, 余叔文.植物适应盐逆境过程中的能量消耗[ J] .植物生理学通讯, 1990(6):70 -73.

相似文献/References:

[1]刘章雄,李卫东,孙石,等.1983～2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
　LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(03):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
　LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(03):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
　WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(03):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
　ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(03):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
　LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(03):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
　DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(03):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
　ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(03):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
　GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(03):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
　WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(03):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
　LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(03):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
[11]张书利,刘丽君,唐晓飞,等.利用农杆菌介导法将BrCS基因导入大豆的研究[J].大豆科学,2011,30(04):569.[doi:10.11861/j.issn.1000-9841.2011.04.0569]
　ZHANG Shu-li,LIU Li-jun,TANG Xiao-fei,et al.Transformation of BrCS Gene into Soybean by Agrobacterium-mediated Method[J].Soybean Science,2011,30(03):569.[doi:10.11861/j.issn.1000-9841.2011.04.0569]
[12]王敏娟,侯文胜,王庆钰,等.过表达GmNHX1基因提高大豆根系的耐盐性[J].大豆科学,2011,30(06):889.[doi:10.11861/j.issn.1000-9841.2011.06.0889]
　WANG Min-juan,HOU Wen-sheng,WANG Qing-yu,et al.Enhancing Salt Tolerance of Soybean Roots by Overexpression of GmNHX1[J].Soybean Science,2011,30(03):889.[doi:10.11861/j.issn.1000-9841.2011.06.0889]
[13]高永,杨静慧,李宏平,等.四种能源植物种子萌芽期的耐盐性研究[J].大豆科学,2010,29(06):1091.[doi:10.11861/j.issn.1000-9841.2010.06.1091]
　GAO Yong,YANG Jing-hui,LI Hong-ping,et al.Salt Tolerance Studies in Four Energy Plants at Germination Period[J].Soybean Science,2010,29(03):1091.[doi:10.11861/j.issn.1000-9841.2010.06.1091]
[14]李冉辉,刘昀,郑易之.大豆PM2蛋白（LEA3）表达可提高酵母重组子的耐盐性[J].大豆科学,2007,26(04):467.[doi:10.3969/j.issn.1000-9841.2007.04.004]
　LI Ran-hui,LIU Yun,ZHENG Yi-zhi.EXPRESSION OF SOYBEAN MP2 PROTEIN(LEA3)CONFERS SALT TOLERANCE IN SACCHAROMYCES CEREVISIAE[J].Soybean Science,2007,26(03):467.[doi:10.3969/j.issn.1000-9841.2007.04.004]
[15]郭蓓邱丽娟邵桂花许占友.大豆耐盐性种质的分子标记辅助鉴定及其利用研究[J].大豆科学,2002,21(01):56.[doi:10.11861/j.issn.1000-9841.2002.01.0056]
　Guo BeiQiu LijuanShao GuihuaXu Zhanyou.MARKERS-ASSISTED IDENTIFICATION OF THE SALT TOLERANTACCESSIONS IN SOYBEAN[J].Soybean Science,2002,21(03):56.[doi:10.11861/j.issn.1000-9841.2002.01.0056]
[16]李大红,蒋炳伸,邬海燕,等.苜蓿MsDREB1基因的诱导表达增强大豆的耐盐性[J].大豆科学,2017,36(01):17.[doi:10.11861/j.issn.1000-9841.2017.01.0017]
　LI Da-hong,JIANG Bing-shen,WU Hai-yan,et al.MsDREB1 Overexpression Improves Tolerance to Salt Stress in Transgenic Glycine max L.[J].Soybean Science,2017,36(03):17.[doi:10.11861/j.issn.1000-9841.2017.01.0017]
[17]牛远,杨修艳,戴存凤,等.大豆芽期和苗期耐盐性评价指标筛选[J].大豆科学,2018,37(02):215.[doi:10.11861/j.issn.1000-9841.2018.02.0215]
　NIU Yuan,YANG Xiu-yan,DAI Cun-feng,et al.Related Indices Selection of Soybean Salt Tolerance at Germination and Seedling Stages[J].Soybean Science,2018,37(03):215.[doi:10.11861/j.issn.1000-9841.2018.02.0215]
[18]杨艳丽,李大红,李鸿雁.过表达桃PpCuZnSOD基因提高大豆耐盐性研究[J].大豆科学,2018,37(04):525.[doi:10.11861/j.issn.1000-9841.2018.04.0525]
　YANG Yan-li,LI Da-hong,LI Hong-yan.Overexpression of PpCuZnSOD Gene Improves Salt Tolerance in Transgenic Soybean[J].Soybean Science,2018,37(03):525.[doi:10.11861/j.issn.1000-9841.2018.04.0525]
[19]王敏,秦杰,杨万明,等.晋大88高匹配性强耐盐根瘤菌筛选[J].大豆科学,2021,40(03):385.[doi:10.11861/j.issn.1000-9841.2021.03.0385]
　WANG Min,QIN Jie,YANG Wan-ming,et al.Screening of Salt-Tolerant and Well Symbiotic Matching Soybean Rhizobia Strains for Jinda 88[J].Soybean Science,2021,40(03):385.[doi:10.11861/j.issn.1000-9841.2021.03.0385]
[20]韩岱,时晓磊,丁孙磊,等.60份大豆种质资源苗期耐盐性鉴定评价[J].大豆科学,2023,42(04):494.[doi:10.11861/j.issn.1000-9841.2023.04.0494]

备注/Memo

基金项目:国家自然科学基金(39870069), 教育部博士点基金(1999002005)和瑞典国际科学基金(IFS)(C3143 -1)资助项目　

作者简介:罗庆云(1974 -), 男, 博士研究生, 主要从事植物逆境生理和遗传研究。

更新日期/Last Update: 2016-12-29