[1]赵洪锟　王玉民　李启云　张明　庄炳昌.中国不同纬度野生大豆和栽培大豆SSR 分析[J].大豆科学,2001,20(03):172-176.[doi:10.11861/j.issn.1000-9841.2001.03.0172]
　Zhao Hong kun Wang Yumin Li Qiyun Zhang Ming Zhuang Bingchang.SSR ANALYSIS OF WILD SOYBEAN (G .soja )AND CULTIVATED SOYBEAN FROMDIFFERENT LATITUDE IN CHINA[J].Soybean Science,2001,20(03):172-176.[doi:10.11861/j.issn.1000-9841.2001.03.0172]
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中国不同纬度野生大豆和栽培大豆SSR 分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第20卷 期数: 2001年03期 页码: 172-176 栏目: 出版日期: 2001-08-25
Title:
SSR ANALYSIS OF WILD SOYBEAN (G .soja )AND CULTIVATED SOYBEAN FROMDIFFERENT LATITUDE IN CHINA
文章编号:
1000 -9841(2001)03 -0172 -05
作者:
赵洪锟　王玉民　李启云　张明　庄炳昌
吉林省农业科学院省农业生物技术重点开放实验室, 公主岭136100
Author(s):
Zhao Hong kun 　Wang Yumin 　Li Qiyun 　Zhang Ming 　Zhuang Bingchang
Ji lin Prov ince Key Lab .On Agro -Biotechnology , JAAS , Gongzhuling , 136100
关键词:
SSR ; 野生大豆; 栽培大豆; 遗传多样性
Keywords:
SSR ; Wild soy bean (G .soja ); Cult ivated soybean (G .max ); Genetic diversity
分类号:
S565.102
DOI:
10.11861/j.issn.1000-9841.2001.03.0172
文献标志码:
A
摘要:
采用SSR 分子标记技术对我国不同纬度的野生大豆(G.soja )和栽培大豆(G.max )各22份进行了多样性分析, 通过对所合成的40 对引物的筛选, 12 对引物扩增结果表现出良好的多态性。引物BARC -sat39 在野生大豆和栽培大豆之间有特异谱带。表明这个SSR 标记是与栽培大豆和野生大豆有关的一个等位基因位点。通过对实验结果量化后数据分析:野生大豆和栽培大豆的平均遗传距离分别为0.176 和0.150 , 表明野生大豆的多态性比栽培大豆较为丰富;在遗传距离0.300 处, 野生大豆和栽培大豆被明显分为二类, 与以往大豆属Soja 亚属的形态学分类结果相一致, 为野生大豆和栽培大豆分为二个种提供了分子水平上的证据。
Abstract:
DNA genetic variation of 22 accessions of wild soy bean and 22 accessions of cultivated soybean , w hichw ere f rom different lat itude in China , w as studied by SSR technique .Fo rty pairs of soybean SS R primers w eresy nthesized according to published data , and 12 out of them had polymorphic alleles.Specific bands were amplified by primer 3 betw een w ild soybean and cultivated soy bean , w hich showed this SS R marker w as one of the allelesamong w ild soybean and cultivated soy bean .The data analysis showed that :The average genet ic distance ofw ild soy bean and cultivated soybean were 0 .176 and 0 .150 respectively which ag reed on the thesis that w ildsoybean had more polymo rphism than that of cultivated soybean .And also it provided a molecular proof thatw ild soybean and cultivated soybean belonged to two species f rom the dendg rom at the site of genetic distance 0 .300 , w hich conformed to phenotypic results .

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备注/Memo

基金项目:国家自然科学基金重点项目(基础上编号:39730330 , 39670448)和“ 973” 资助项目。　

作者简介:赵洪锟(1974 -), 女, 研实, 现从事大豆分子生物学研究。

更新日期/Last Update: 2016-12-29