[1]杨艳丽,李大红,李鸿雁.过表达桃PpCuZnSOD基因提高大豆耐盐性研究[J].大豆科学,2018,37(04):525-530.[doi:10.11861/j.issn.1000-9841.2018.04.0525]
　YANG Yan-li,LI Da-hong,LI Hong-yan.Overexpression of PpCuZnSOD Gene Improves Salt Tolerance in Transgenic Soybean[J].Soybean Science,2018,37(04):525-530.[doi:10.11861/j.issn.1000-9841.2018.04.0525]
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过表达桃PpCuZnSOD基因提高大豆耐盐性研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第37卷 期数: 2018年04期 页码: 525-530 栏目: 出版日期: 2018-07-20
Title:
Overexpression of PpCuZnSOD Gene Improves Salt Tolerance in Transgenic Soybean
作者:
杨艳丽; 李大红; 李鸿雁
（黄淮学院 生物与食品工程学院，河南 驻马店 463000）
Author(s):
YANG Yan-li;  LI Da-hong; LI Hong-yan
(School of Biotechnology and Food Engineering, Huanghuai University, Zhumadian 463000, China)
关键词:
过表达; PpCuZnSOD; 耐盐性; 大豆
Keywords:
Overexpression;  PpCuZnSOD;  Salt tolerance;  Soybean
DOI:
10.11861/j.issn.1000-9841.2018.04.0525
文献标志码:
A
摘要:
为研究桃（Prunus persica L.）自由基清除剂CuZnSOD编码基因对于大豆耐盐性的促进作用，将桃PpCuZnSOD全长基因克隆到连有35S启动子的表达载体上，并通过农杆菌导入大豆基因组中，通过PCR和Southern杂交方法检测阳性转基因植株，并对T2代转基因植株进行基因表达量分析和耐盐性评估。结果表明：转基因植株有较高的耐盐性，表现为种子萌发率和存活率高，叶绿素含量高。转基因植株在盐胁迫下存活时间更长，与非转基因植株相比，转基因植株的SOD、POD、CAT酶活性较高，而丙二醛含量显著降低。研究结果表明PpCuZnSOD基因过表达可以显著提高大豆植株的SOD活性水平，缓解盐胁迫对大豆的氧化损伤。
Abstract:
In order to study the enhanced effect of CuZnSOD encoding gene of peach on salt tolerance of soybean, a full-length cDNA of a CuZnSOD from peach(Prunus persica L.) PpCuZnSOD was cloned, and the target gene is introduced into the soybean genome via Agrobacterium tumefaciens. The transgenic plants was detected by PCR, Southern blot and quantitative real time PCR. Two transgenic soybean lines of T2 generation were recruited and treated with 150 mmol·L-1 NaCl, which simulating salt conditions. The results showed that transgenic soybean plants had high salt tolerance. The seed germination rates, survival rate and the contents of chlorophyll were higher than that of wild-type. Transgenic plants had more high SOD, POD and CAT activities, while less MDA content under salt stress compared with wild-type plants. Our results substantiate that increased levels of SOD activity brought about by overexpression of PpCuZnSOD gene may play an important role in ameliorating oxidative injury induced by salt stress.

参考文献/References:

［1］Chen X, Chen Z, Zhao H, et al. Genome-wide analysis of soybean HD-Zip gene family and expression profiling under salinity and drought treatments［J］. PloS One, 2014, 9(2): e87156.
［2］Jaspers P, Kangasjarvi J. Reactive oxygen species in abiotic stress signaling［J］. Physiology Plant, 2010, 138(4): 405-413.
［3］Sharma P, Jha A B, Dubey R S, et al. Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions［J］. Journal of Botany, 2012, 12(24): 1-26.
［4］Negi N P, Shrivastava D C, Sharma V,et al. Overexpression of CuZnSOD from Arachis hypogaea alleviates salinity and drought stress in tobacco［J］. Plant Cell Reports, 2015, 34(7):1109-1126.?
［5］Yan H, Li Q, Park S C,et al. Overexpression of CuZnSOD and APX enhance salt stress tolerance in sweet potato［J］. Plant Physiology and Biochemistry, 2016, 109(1): 20-27.?
［6］Noctor G, Mhamdi A, Chaouch S, et al. Glutathione in plants: An integrated overview［J］. Plant, Cell & Environment, 2012, 35(2): 454-484.?
［7］Gill S S, Tuteja N. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants［J］. Plant Physiology and Biochemistry, 2010, 48(12): 909-930.
［8］Mittler R, Vanderauwera S, Gollery M,et al. Reactive oxygen gene network of plants［J］. Trends Plant Science, 2004, 9(10):490-498.
［9］Hwang C S, Rhie G, Oh J H,et al. Copper- and zinc-containing superoxide dismutase (Cu/ZnSOD) is required for the protection of Candida albicans against oxidative stresses and the expression of its full virulence［J］. Microbiology, 2002, 148(11): 3705-3713.
［10］Hernandez J A, Almansa M S. Short term effects of salt stress on antioxidant system and leaf water relations of pea leaves［J］. Physiology Plant, 2002, 115(2): 251-257.
［11］Xu J, Duan X, Yang J,et al. Coupled expression of Cu/Zn-superoxide dismutase and catalase in cassava improves tolerance against cold and drought stresses［J］. Plant Signaling and Behavior, 2013, 8(6): 24-25.
［12］Tarchoune I, Sgherri C, Izzo R,et al. Antioxidative responses of Ocimum basilicum to sodium chloride or sodium sulphate salinization［J］. Plant Physiology and Biochemistry, 2010, 48(1): 772-777.
［13］Dionisio-Sese M L, Tobita S. Antioxidant responses of rice seedlings to salinity stress［J］. Plant Science, 1998, 135(1): 1-9.
［14］Guan Q, Liao X, He M,et al. Tolerance analysis of chloroplast OsCu/Zn-SOD overexpressing rice under NaCl and NaHCO3 stress［J］. PloS One, 2017, 12(10): e0186052.?
［15］Zhou Y, Hu L, Wu H,et al. Genome-wide identification and tanscriptional expression analysis of cucumber superoxide dismutase (SOD) family in response to various abiotic stresses［J］. International Journal of Genomics, 2017, 2017: 1-14.?
［16］Shiriga K, Sharma R, Kumar K,et al. Expression pattern of superoxide dismutase under drought stress in Maize［J］. International Journal of Innovative Research in Science, 2014, 3(4): 11333-11337.
［17］Westgate M E, Peterson C M. Flower and pod development in water-deficient soybeans (Glycinemax L Merr) ［J］. Journal of Experimental Botany, 1993, 44(258): 109-117.
［18］Murraym G, Thompson W F. Rapid isolation of high molecular weight plant DNA［J］. Nucleic Acids Research, 1980, 8(19): 4321-4325.
［19］Li D H, Liu H, Yang Y L,et al. Down-regulated expression of RACK1 gene by RNA interference enhances drought tolerance in rice［J］. Rice Science, 2009, 16(1): 14-20.
［20］高俊凤. 植物生理学实验指导［M］. 北京: 高等教育出版社, 2006: 211-213, 217.(Gao J F. Experimental instruction of plant physiology［M］. Beijing: Higher Education Press, 2006: 211-213, 217.)
［21］Lee Y P, Ahmad R, Lee H S,et al. Improved tolerance of Cu/Zn superoxide dismutase and ascorbate peroxidase expressing transgenic tobacco seeds and seedlings against multiple abiotic stresses［J］. International Journal of Agriculture and Biology, 2013, 15(4): 725-730.
［22］Shafi A, Chauhan R, Gill T,et al. Expression of SOD and APX genes positively regulates secondary cell wall biosynthesis and promotes plant growth and yield in Arabidopsis under salt stress［J］. Plant Molecular Biology, 2015, 87(6): 615-631.
［23］Alscher R G, Erturk N, Heath L S. Role of superoxide dismutases (SODs) in controlling oxidative stress in plants［J］. Journal of Experimental Botany, 2002, 53(372): 1331-1341.
［24］Leclercq J, Martin F, Sanier C,et al. Overexpression of a cytosolic isoform of the HbCuZnSOD gene in Hevea brasiliensis changes its response to a water deficit［J］. Plant Molecular Biology, 2012, 80(3):255-272.
［25］Sharma P, Jha A B, Dubey R S, et al. Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions［J］. Journal of Botany, 2012, 12(1):1-26.

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备注/Memo

收稿日期：2018-03-31

基金项目：河南省科技计划项目（182102110305）；河南省高等学校重点项目（16A210032）。
第一作者简介：杨艳丽（1979-），女，学士，实验师，主要从事园林技术及植物生理研究开发工作。E-mail:1654460639@qq.com。
通讯作者：李鸿雁（1967-），女，硕士，教授，主要从事园林植物与逆境生理研究工作。E-mail:1248762303@qq.com。

更新日期/Last Update: 2018-07-31