[1]郭蓓邱丽娟邵桂花许占友.大豆耐盐性种质的分子标记辅助鉴定及其利用研究[J].大豆科学,2002,21(01):56-61.[doi:10.11861/j.issn.1000-9841.2002.01.0056]
　Guo BeiQiu LijuanShao GuihuaXu Zhanyou.MARKERS-ASSISTED IDENTIFICATION OF THE SALT TOLERANTACCESSIONS IN SOYBEAN[J].Soybean Science,2002,21(01):56-61.[doi:10.11861/j.issn.1000-9841.2002.01.0056]
点击复制

大豆耐盐性种质的分子标记辅助鉴定及其利用研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第21卷 期数: 2002年01期 页码: 56-61 栏目: 出版日期: 2002-02-25
Title:
MARKERS-ASSISTED IDENTIFICATION OF THE SALT TOLERANTACCESSIONS IN SOYBEAN
文章编号:
1000 -9841(2002)01 -0056 -06
作者:
郭蓓1邱丽娟2邵桂花3许占友2
1.北京农学院北京102206 ;
2.中国农科院作物品种资源研究所北京100081 ;
3.中国农科院作物育种栽培研究所北京100081
Author(s):
Guo Bei1Qiu Lijuan2Shao Guihua3Xu Zhanyou2
1.Beij ing Agricultural College , Beij ing 102206 ;
2.Insti tute of Crop Germplasm Resources ,CA AS , Beij ing 100081 ;
3.Inst itute of Crop Breeding and Cul tiv ation , CA AS , Bei jing 100081
关键词:
大豆; 耐盐性; 分子标记; 种质
Keywords:
Soybean ; Salt tolerance ; Molecular markers; Accessions
分类号:
S 565.103.4
DOI:
10.11861/j.issn.1000-9841.2002.01.0056
文献标志码:
A
摘要:
大豆耐盐种质的鉴定对于促进大豆耐盐育种具有重要作用。本文利用已获得的大豆耐盐性基因的共显性PCR 标记, 对选自于国家作物种质库的59 份耐盐和盐敏感种质加以鉴定, 同时进行了田间耐盐性重复鉴定, 并对种质库耐盐性记载结果、田间耐盐性重复鉴定结果与分子标记鉴定结果进行比较, 从中选出田间耐盐性重复鉴定结果与分子标记鉴定结果一致的耐盐种质42 份, 分析了这些种质间的遗传多样性, 为大豆种质资源的改良及耐盐遗传育种中的亲本选配提供了理论依据, 同时对分子标记应用于种质鉴定和育种实践进行了有益的尝试。
Abstract:
The identif ication of soybean tolerant accessions play a important role in soybean breeding .Themolecular markers of salt tolerant gene loci w ere used to identify 59 salt tolerant and susceptible accessions ofsoybean selected from the National Gene Bank .The 42 tolerant accessions w ith the same result between the re -identification in the field and the identification w ith marker were selected out through comparing among therecords fo r salt tolerance in Gene Bank , the re -identification in the field and the identification w ith marker ,and the genetic diversity of these accessions were analy zed .It has provided the valuable information for selectingsalt tolerant parent in improving cultivars and generating novel lines in soybean breeding .Meanw hile , it madeuseful ex plo ration on the application of molecular markers to accessions identif icat ion and breeding .

参考文献/References:

1 　Weisemann J M , Mat thews B F , Devine T E .Molecular markers located proximal to the soybean cyst nematode resist ance gene, Rhg4[ J] .Theor Appl Genet , 1992 , 85 :136-138.

2 　Concibido V C , Denny R L , Bout in S R.DNA marker analysis ofloci underlying resist ance to soybean cyst nematode (Heteroderaglycines i chinohe)[ J] .Crop Science, 1994 , 34 :240-246.
3 　Mahalingam R.DNA markers for resist ance to Heterodera glycinesrace3 in soybean Peking[ J] .Breeding Science, 1995 , 45 :435-443.
4 　颜清上, 王岚, 李莹.利用RAPD 技术寻找大豆抗孢囊线虫4 号小种标记初报[ J] .大豆科学, 1996 , 15(2):126-129.
5 　Yu Y G, Saghai Maroof M A , Buss G R.RFLP and microsatellitemapping of a gene for soybean mosaic vi rus resist ance [ J] .Phytopathology, 1993 , 84(1):60-64.
6 　张志永, 陈受宜, 盖钧镒.大豆花叶病毒抗性基因Rsa 的分子标记[ J] .科学通报, 1998 , 43(20):2197-2201.
7 　Zheng C M , Chang RZ , Qiu L J .T agging resist ant gene of SMV bymolecular markers [ J] .Proceeding of World Soybean Research ConferenceVI, 1999 , 432.
8 　邹继军, 董伟, 杨庆凯.大豆对灰斑病菌7 号小种抗性的遗传分析及抗病基因的RAPD 标记[ J] .科学通报, 1998 , 43 (21):2302-2307.
9 　Yu Y G , Saghai Maroof M A, Buss G R.Divergence and allelomorphicrelat ionship of a soybean virus resist ance gene based on tight lylinked DNA microsat ellite and RFLP markers [ J] .T heor ApplGenet , 1996 , 92 :64-69.
10 邹继军, 杨庆凯, 陈受宜.大豆灰斑病抗病基因RAPD 标记的分子特征及抗感种质的SCAR 标记鉴定[ J] .科学通报, 1999 , 44(23):2544-2550.
11 Guo Bei , Shao G H , Chang R Z .A RAPD marker linked to the salttolerant gene in soybean[ J] .S oybean Genet ics New slett er , 1998 ,25 :30-31.
12 郭蓓, 邱丽娟, 邵桂花.大豆耐盐基因的PCR 标记[ J] .中国农业科学, 2000 , 33(1):10 ～ 16.
13 邵桂花.大豆种质资源耐盐性田间鉴定方法[ J] .作物杂志,1987 , (3):36-37.
14 邵桂花, 宋景芝, 刘惠令.大豆种质资源耐盐性鉴定初报[ J] .中国农业科学, 1986 , (6):30-34.
15 Fehr W R.Breeding methods f or cul tivar developm ent .In B.E .Caldwell (ed .)Soybeans :Improvement , production and uses[ J] .Agronomy, 1987 , 16 :249-294.
16 Thompson and Nelson .Ut ilization of di versit y germplasm f or soybeanyield improvement[ J] .Crop Science, 1998 , 38 :1362-1368.
17 Diers B W , Mansur L , Imsande J .Mapping Phyt ophthora resist anceloci in soybean wi th rest riction f ragment length polymorphism markers[J] .Crop Science, 1992 , 32 :377-383.

相似文献/References:

[1]刘章雄,李卫东,孙石,等.1983～2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
　LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
　LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
　WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
　ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
　LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
　DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
　ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
　GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
　WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
　LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
[11]张书利,刘丽君,唐晓飞,等.利用农杆菌介导法将BrCS基因导入大豆的研究[J].大豆科学,2011,30(04):569.[doi:10.11861/j.issn.1000-9841.2011.04.0569]
　ZHANG Shu-li,LIU Li-jun,TANG Xiao-fei,et al.Transformation of BrCS Gene into Soybean by Agrobacterium-mediated Method[J].Soybean Science,2011,30(01):569.[doi:10.11861/j.issn.1000-9841.2011.04.0569]
[12]王敏娟,侯文胜,王庆钰,等.过表达GmNHX1基因提高大豆根系的耐盐性[J].大豆科学,2011,30(06):889.[doi:10.11861/j.issn.1000-9841.2011.06.0889]
　WANG Min-juan,HOU Wen-sheng,WANG Qing-yu,et al.Enhancing Salt Tolerance of Soybean Roots by Overexpression of GmNHX1[J].Soybean Science,2011,30(01):889.[doi:10.11861/j.issn.1000-9841.2011.06.0889]
[13]高永,杨静慧,李宏平,等.四种能源植物种子萌芽期的耐盐性研究[J].大豆科学,2010,29(06):1091.[doi:10.11861/j.issn.1000-9841.2010.06.1091]
　GAO Yong,YANG Jing-hui,LI Hong-ping,et al.Salt Tolerance Studies in Four Energy Plants at Germination Period[J].Soybean Science,2010,29(01):1091.[doi:10.11861/j.issn.1000-9841.2010.06.1091]
[14]李冉辉,刘昀,郑易之.大豆PM2蛋白（LEA3）表达可提高酵母重组子的耐盐性[J].大豆科学,2007,26(04):467.[doi:10.3969/j.issn.1000-9841.2007.04.004]
　LI Ran-hui,LIU Yun,ZHENG Yi-zhi.EXPRESSION OF SOYBEAN MP2 PROTEIN(LEA3)CONFERS SALT TOLERANCE IN SACCHAROMYCES CEREVISIAE[J].Soybean Science,2007,26(01):467.[doi:10.3969/j.issn.1000-9841.2007.04.004]
[15]罗庆云　於丙军　刘友良.大豆苗期耐盐性鉴定指标的检验[J].大豆科学,2001,20(03):177.[doi:10.11861/j.issn.1000-9841.2001.03.0177]
　Luo Qingyun Yu Bingjun Liu Youliang.EFFECTOF NaCl ON THE GROWTH,K+ ,Na+ AND Cl -DISTRIBUTION INSEEDLINGS OF 6 SOYBEAN CULTIVARS(Glycine max L.Merril l)[J].Soybean Science,2001,20(01):177.[doi:10.11861/j.issn.1000-9841.2001.03.0177]
[16]李大红,蒋炳伸,邬海燕,等.苜蓿MsDREB1基因的诱导表达增强大豆的耐盐性[J].大豆科学,2017,36(01):17.[doi:10.11861/j.issn.1000-9841.2017.01.0017]
　LI Da-hong,JIANG Bing-shen,WU Hai-yan,et al.MsDREB1 Overexpression Improves Tolerance to Salt Stress in Transgenic Glycine max L.[J].Soybean Science,2017,36(01):17.[doi:10.11861/j.issn.1000-9841.2017.01.0017]
[17]牛远,杨修艳,戴存凤,等.大豆芽期和苗期耐盐性评价指标筛选[J].大豆科学,2018,37(02):215.[doi:10.11861/j.issn.1000-9841.2018.02.0215]
　NIU Yuan,YANG Xiu-yan,DAI Cun-feng,et al.Related Indices Selection of Soybean Salt Tolerance at Germination and Seedling Stages[J].Soybean Science,2018,37(01):215.[doi:10.11861/j.issn.1000-9841.2018.02.0215]
[18]杨艳丽,李大红,李鸿雁.过表达桃PpCuZnSOD基因提高大豆耐盐性研究[J].大豆科学,2018,37(04):525.[doi:10.11861/j.issn.1000-9841.2018.04.0525]
　YANG Yan-li,LI Da-hong,LI Hong-yan.Overexpression of PpCuZnSOD Gene Improves Salt Tolerance in Transgenic Soybean[J].Soybean Science,2018,37(01):525.[doi:10.11861/j.issn.1000-9841.2018.04.0525]
[19]王敏,秦杰,杨万明,等.晋大88高匹配性强耐盐根瘤菌筛选[J].大豆科学,2021,40(03):385.[doi:10.11861/j.issn.1000-9841.2021.03.0385]
　WANG Min,QIN Jie,YANG Wan-ming,et al.Screening of Salt-Tolerant and Well Symbiotic Matching Soybean Rhizobia Strains for Jinda 88[J].Soybean Science,2021,40(01):385.[doi:10.11861/j.issn.1000-9841.2021.03.0385]
[20]韩岱,时晓磊,丁孙磊,等.60份大豆种质资源苗期耐盐性鉴定评价[J].大豆科学,2023,42(04):494.[doi:10.11861/j.issn.1000-9841.2023.04.0494]

备注/Memo

作者简介:郭蓓(1962 -), 女, 硕士, 讲师, 研究方向分子标记。

更新日期/Last Update: 2016-12-29