[1]丁德荣　盖钧镒.大豆质核互作雄性不育系NJCMS1A 花粉败育的细胞学研究[J].大豆科学,2001,20(03):167-171.[doi:10.11861/j.issn.1000-9841.2001.03.0167]
　Ding Derong Gai Juny i.CYTOLOGICAL STUDIES ON POLLEN ABORTION INCYEOPLASMIC-NUCLEARMALE STERILE SOYBEAN LINS NJCMS1A[J].Soybean Science,2001,20(03):167-171.[doi:10.11861/j.issn.1000-9841.2001.03.0167]
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大豆质核互作雄性不育系NJCMS1A 花粉败育的细胞学研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第20卷 期数: 2001年03期 页码: 167-171 栏目: 出版日期: 2001-08-25
Title:
CYTOLOGICAL STUDIES ON POLLEN ABORTION INCYEOPLASMIC-NUCLEARMALE STERILE SOYBEAN LINS NJCMS1A
文章编号:
1000 -9841(2001)03 -0167 -05
作者:
丁德荣　盖钧镒
南京农业大学大豆研究所;农业部国家大豆改良中心南京210095
Author(s):
Ding Derong 　Gai Juny i
Soybean Research Insti tute , Nanj ing Agricul tural University ;National Center ofSoybean Improvement , Minist ry of Agriculture , Nanjing 210095
关键词:
大豆; 质核互作雄性不育; 小孢子发育; 细胞色素氧化酶
Keywords:
Soybean ; Cy toplasmic -nuclear male sterility ; Microspore development ; Cy tochrome oxidase
分类号:
S565.101
DOI:
10.11861/j.issn.1000-9841.2001.03.0167
文献标志码:
A
摘要:
利用光镜和透射电镜对(N8855 ×N2899)F1 不育株和(N2899 ×N8855)F1 可育株、BC1F1 不育株、质核互作不育系NJCMS1A 和保持系NJCMS1B 进行小孢子发育的细胞学比较观察。结果表明, 同一胞质在不同的回交世代败育途径和表现相似, 主要发生在单核后期到二胞花粉早期:(N8855 ×N2899)F1 与BC1F1 不育花药在小孢子单核靠边期细胞质产生不正常空泡, 至近成熟花粉时细胞质部分解体、收缩;NJCMS1A 花粉败育发生在二胞花粉期, 初期原生质内小液泡比保持系增多, 原生质内未形成淀粉粒, 在生殖细胞远离营养细胞后, 胞质呈现紊乱, 生殖核与营养核消失, 外壁内层及内壁停止发育, 液泡增大, 原生质部分解体。分别与反交F1及保持系比较, 处于二胞花粉时期的正交F1 及不育系细胞色素氧化酶最快迁移活性带缺失, 此特征带缺失与不育花药败育时期基本同步。
Abstract:
Comparative observation on microspore development in cy toplasmic -nuclear male sterile soybean material, such as (N8855 ×N2899)F1 , BC1 male sterile plant s , NJCMS1A and i ts fertile countpart (N2899 ×N8855)F1 , maintainer line , w as made w ith light and transmission electron microscopy .The result show ed theabortion appearance of the dif ferent generatio ns with the same cy toplasm w as similar , pollen abo rtion occurredmajorly f rom late mononucleate stage to binucleate stage .The abno rmal vacuoles in microspo re in (N8855 ×N2899)F1 male sterile anther occurred at late mononucleate microspore stage .Plasmodium shriveled at maturepollen g rain , The partial cy toplasm disinterg rated , and 2 -3 nucleus w ere found in some microspores in BC1sterile plants .Pollen abortion of NJCMS1A occurred at the stage of binucleate pollen .There appeared mo resmall vacuoles in microspo re cy toplasm of NJCMS1A than that of it s maintainer line at the stage that generativecell closed to vegetative cell , starch grains not being produced in cytoplasm .After generative cell w as aw ay f romvegetative cell , part of cytoplasm disordered , generative and vegetative nuclei further degenerated , vacuoles expanded, part of cy toplasm disinteg rated , and the development of endexine w as completely inhibited .Isozymeanaly sis indicated that the fastest cytochrome oxidase active band missed at binucleate stage , w hich w as consistentw ith the result of the pollen abortion phase .Thus , the mechanism of the cytoplasmic -nuclear male sterilityw as probably associated w ith cy tochrome o xidase .

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备注/Memo

基金项目:国家自然科学基金(9390013)和国家攻关项目(96 -002 -02 -12 -4)。　

作者简介:丁德荣(1967 -), 博士, 现为复旦大学遗传研究所博士后研究人员。

更新日期/Last Update: 2016-12-29