[1]杨龙树,李佳佳,贺亭亭,等.大豆质核互作雄性不育系NJCMS1A与其保持系NJCMS1B的生理生化特性比较分析[J].大豆科学,2017,36(03):391-398.[doi:10.11861/j.issn.1000-9841.2017.03.0391]
　YANG Long-shu,LI Jia-jia,HE Ting-ting,et al.Comparative Analysis of Physiological and Biochemical Characteristics Between CytoplasmicNuclear Male Sterile Line and Its Maintainer in Soybean ［Glycine max(L) Merr］[J].Soybean Science,2017,36(03):391-398.[doi:10.11861/j.issn.1000-9841.2017.03.0391]
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大豆质核互作雄性不育系NJCMS1A与其保持系NJCMS1B的生理生化特性比较分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第36卷 期数: 2017年03期 页码: 391-398 栏目: 出版日期: 2017-05-20
Title:
Comparative Analysis of Physiological and Biochemical Characteristics Between CytoplasmicNuclear Male Sterile Line and Its Maintainer in Soybean ［Glycine max(L) Merr］
作者:
杨龙树1; 李佳佳1; 2; 贺亭亭1; 丁先龙1; 张浩1; 韩少怀1; 盖钧镒1; 杨守萍1
（1.南京农业大学 大豆研究所/国家大豆改良中心/农业部大豆生物学与遗传育种重点实验室（综合）/作物遗传与种质创新国家重点实验室/江苏省现代作物生产协同创新中心，江苏 南京 210095； 2.安徽农业大学 农学院，安徽 合肥 230036）
Author(s):
YANG Long-shu1;  LI Jia-jia1; 2;  HE Ting-ting1;  DING Xian-long1;  ZHANG Hao1; HAN Shao-huai1;  GAI Jun-yi1;  YANG Shou-ping1
（1.Soybean Research Institute, Nanjing Agricultural University/National Center for Soybean Improvement/Key Laboratory for Biology and Genetic.Improvement of Soybean (General),Ministry of Agriculture/State Key Laboratory of Crop Genetics and Germplasm Enhancement/Jiangsu Modern Crop Production Collaborative Innovation Center, Nanjing 210095, China; 2.College of Agriculture, Anhui Agricultural University, Hefei 230036, China）
关键词:
大豆; 质核互作雄性不育; 生理生化特性
Keywords:
Soybean ［Glycine max (L) Merr］;  Cytoplasmic-nuclear male sterility (CMS);  Total sugar content;  Enzyme activity
DOI:
10.11861/j.issn.1000-9841.2017.03.0391
文献标志码:
A
摘要:
以大豆质核互作雄性不育系NJCMS1A及其保持系NJCMS1B的花芽为试验材料，利用试剂盒法和紫外分光光度计测定分析了糖分含量（可溶性糖和淀粉）和总ATPase、蔗糖磷酸合成酶（SPS）、磷酸烯醇式丙酮酸羧激酶 (PEPCK)、抗坏血酸氧化酶(AO)、过氧化氢酶（CAT）、超氧化物歧化酶（SOD）以及过氧化物酶（POD）等酶活性的变化情况。结果表明:相较于保持系NJCMS1B，总ATPase、PEPCK、AO和CAT活性在不育系NJCMS1A中显著下降；糖分含量、SPS和SOD活性在不育系NJCMS1A中下降水平不显著；POD活性在不育系NJCMS1A中显著上升。根据结果分析推测，与能量代谢或胁迫响应等相关的物质或酶活性在不育系NJCMS1A中的亏损现象可能是NJCMS1A花粉败育的主要原因。
Abstract:
f the cytoplasmic-nuclear male sterile line NJCMS1A and its maintainer NJCMS1B of soybean as test materials，the content of sugar (soluble sugar and starch)，the activities of total ATPase, sucrose phosphate synthase (SPS), phosphoenolpyruvate carboxykinase (PEPCK), ascorbate oxidase (AO), catalase (CAT), superoxide dismutase (SOD) and peroxidase (POD) were measured using the kit method and UV-spectrophotometer.The results showed that the total ATPase, PEPCK, AO and CAT activity were significantly decreased and total sugar content, SPS, and POD activity non-remarkably decreased in the male sterile line NJCMS1A, compared with its maintainer NJCMS1B; the activity of POD showed a significant increase in the male sterile line NJCMS1A, compared with its maintainer NJCMS1B.According to the present analysis results, we concluded that the deficiency phenomenon of substances or enzymes activity associated with energy metabolism or stress response in male sterile line NJCMS1A of soybean, may be the main reason for poller abortion

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备注/Memo

基金项目：国家重点研发计划（2016YFD0101500，2016YFD0101504）；国家高技术研究发展计划 “863计划”（2011AA10A105）；教育部创新团队发展计划（PCSIRT13073）。

第一作者简介：杨龙树（1992-），男，硕士，主要从事大豆分子遗传育种研究。E-mail：dashu6090@163.com。
通讯作者：杨守萍（1967-），女，博士，教授，主要从事大豆遗传育种研究。E-mail: spyung@126.com。

更新日期/Last Update: 2017-06-26