[1]董建生,杨守萍,喻德跃,等.大豆质核互作雄性不育系NJCMS2A的育性恢复性遗传和育性恢复基因的SSR标记[J].大豆科学,2008,27(02):181-185.[doi:10.11861/j.issn.1000-9841.2008.02.0181]
　DONG Jian-sheng,YANG Shou-ping,YU De-yue,et al.Inheritance and Gene Tagging of Male Fertility Restoration of Cytoplasmic-NuclearMale Sterile Line NJCMS2A in Soybean[J].Soybean Science,2008,27(02):181-185.[doi:10.11861/j.issn.1000-9841.2008.02.0181]
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大豆质核互作雄性不育系NJCMS2A的育性恢复性遗传和育性恢复基因的SSR标记

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第27卷 期数: 2008年02期 页码: 181-185 栏目: 出版日期: 2008-04-30
Title:
Inheritance and Gene Tagging of Male Fertility Restoration of Cytoplasmic-NuclearMale Sterile Line NJCMS2A in Soybean
文章编号:
1000-9841(2008)02-0181-05
作者:
董建生; 杨守萍; 喻德跃; 盖钧镒
南京农业大学大豆研究所，国家大豆改良中心，作物遗传与种质创新国家重点实验室，江苏 南京 210095
Author(s):
DONG Jian-sheng; YANG Shou-ping; YU De-yue; GAI Jun-yi
Nanjing Agricultural University，Soybean Research Institute，National Center for Soybean Improvement，National Key Laboratory of Crop Genetics and Germplasm Enhancement，Nanjing 210095，Jiangsu，China
关键词:
大豆; 质核互作雄性不育; 遗传; SSR标记
Keywords:
Soybean; Cytoplasmic-nuclear male-sterility; Inheritance; SSR marker
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2008.02.0181
文献标志码:
A
摘要:
利用组合NJCMS2A×中豆5号对大豆质核互作雄性不育系NJCMS2A的育性恢复性遗传进行研究，结果表明：NJCMS2A的育性恢复性遗传在不同年份间表现稳定，F1全部为可育株，F1∶2表现育性分离，可育株与不育株的分离比例经χ2测验符合15∶1，F2∶3中无育性分离的家系数∶分离比例符合(3∶1)的家系数∶分离比例符合(15∶1)的家系数经χ2测验符合7∶4∶4，说明在组合NJCMS2A×中豆5号中NJCMS2A的育性恢复性由两对显性重叠基因控制，验证了Bai和Gai的研究结果。随机选取组合NJCMS2A×中豆5号的一个F1∶2株行群体作为分子标记定位群体，采用903对大豆SSR引物对NJCMS2A育性恢复基因进行分子标记和定位，结果找到一个SSR标记Satt135与NJCMS2A育性恢复基因连锁，采用极大似然法计算重组率，利用Kosambi函数将重组率转化为遗传距离，得到Satt135与NJCMS2A育性恢复基因的遗传距离为11.47 cM，参照Song等整合的大豆分子遗传图谱，将NJCMS2A育性恢复基因定位于D2连锁群上。
Abstract:
Cytoplasmic-nuclear male sterility plays an important role in the production of hybrid seeds.The knowledge of inheritance of male fertility restoration of a cytoplasmic-nuclear male-sterile line is essential to hybrid seed production.With the rapid development of molecular markers，mapping male fertility restoration genes appears an effective way to study inheritance of the trait.The soybean cytoplasmic-nuclear male-sterile line NJCMS2A was developed through consecutive backcross procedures with the F2 male sterile plants of the N8855(N1628 cross as donor parent and N1628 (designated as NJCMS2B afterwards) as recurrent parent.In the present paper，the inheritance of the male fertility restoration of NJCMS2A was studied，at the same time，the SSR(simple sequence repeat)marker linked to the restorer gene of NJCMS2A was screened.The results showed that the inheritance of the fertility restoration of NJCMS2A was steady among different years.All F1plants of the NJCMS2A×Zhongdou 5 cross were male-fertile.The F1∶2population exhibited male fertility segregation，and the segretation ratio of male-fertile to male-sterile plants fitted a 15∶1 ratio under χ2 test.The F1and F1∶2results proposed a possible inheritance model of two duplicate dominant genes.The segregation ratio of number of non-segregating male-fertile F2∶3families∶number of 3∶1 segregation F2∶3families∶number of 15∶1 segregation F2∶3families fitted a 7∶4∶4 ratio by χ2 test as expected.All the above phenotypic and genotypic segregation data demonstrated the hypothesis that male fertility restoration of NJCMS2A was controlled by two pairs of duplicate dominant genes，which further verified the results of Bai and Gai.One F1：2population from the NJCMS2A×Zhongdou 5 cross was chosen randomly to tag the restorer gene of NJCMS2A with 903 pairs of SSR primers selected randomly from the soybean public genetic linkage map reported by Cregan et al.The result showed that one SSR marker，e.g.Satt135 was linked to the restorer gene of NJCMS2A.The recombination ratio of the restorer gene of NJCMS2A with Satt135 calculated from the maximum likelihood method was 0.1127，and converted into Kosambi’s genetic distance，was 11.47cM.In comparison with the integrated linkage map by Song et al.，the restorer gene of NJCMS2A was located on the linkage group D2.

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备注/Memo

基金项目：国家自然科学基金资助项目(30490250，30671314)；国家重点基础研究发展计划资助项目(2002CB111304，2004CB117206，2006CB101708)；国家高技术研究发展计划资助项目(2002AA211052，2006AA100104, 2006AA10Z1C1)；长江学者和创新团队发展计划资助项目(PCSIRT)。

作者简介：董建生（1972-），男，硕士研究生，研究方向为植物遗传育种。

更新日期/Last Update: 2014-10-10