[1]宰学明　吴国荣　陆长梅　顾龚平　王娜.低温预处理对大豆萌芽活力及其活性氧代谢的影响[J].大豆科学,2001,20(03):163-166.[doi:10.11861/j.issn.1000-9841.2001.03.0163]
　Zai Xueming Wu Guorong Lu ChangmeiGu Gongping Wang Na.THE EFFECTS OF PRECHILLINGON VIGOUR INDESAND ACTIVE OXYGEN METABOLISMOF SOYBEAN SEEDS[J].Soybean Science,2001,20(03):163-166.[doi:10.11861/j.issn.1000-9841.2001.03.0163]
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低温预处理对大豆萌芽活力及其活性氧代谢的影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第20卷 期数: 2001年03期 页码: 163-166 栏目: 出版日期: 2001-08-25
Title:
THE EFFECTS OF PRECHILLINGON VIGOUR INDESAND ACTIVE OXYGEN METABOLISMOF SOYBEAN SEEDS
文章编号:
1000 -9841(2001)03 -0163 -04
作者:
宰学明　吴国荣　陆长梅　顾龚平　王娜
南京师范大学生命科学学院南京210097
Author(s):
Zai Xueming 　Wu Guorong 　Lu Changmei　Gu Gongping 　Wang Na
The College of Li fe Science , Nanjing Normal Universi ty , Nanjing 210097
关键词:
大豆种子; 低温; 活力指数; 活性氧代谢
Keywords:
Soybean seed ; Prechilling treatment ; Vigour index ; Active oxy gen metabolism
分类号:
S565.01
DOI:
10.11861/j.issn.1000-9841.2001.03.0163
文献标志码:
A
摘要:
采用低温(3 -5 ℃)处理大豆种子(黑脐91 -1)能显著提高种子萌发率和萌芽活力, 其中以处理15 天的效果更为明显。在大豆萌发过程中, 低温处理组的外渗电导率明显较对照组低, 呼吸速率升高的速度较对照组更快, 超氧物岐化酶(SOD)和过氧化物酶(POD)的活性均显著高于对照,超氧阴离子(O2-)含量和脂质过氧化产物丙二醛(MDA)的积累量均明显低于对照。表明种子萌发前经低温预处理能有效提高大豆种子萌发时活性氧代谢水平, 有利于好苗、壮苗的形成。
Abstract:
Soybean seeds w ere t reated w ith prechilling (3 -5 ℃).Results showed that the germination rate andvigour index of soybean seeds treated increased notably , the t reatment w ith prechilling for 15 day s w as the mosteffective .During germinating period , the conductivity rate w as lower and the respiratory intensity increasedmore rapidly than that of the control , the activi ty of superoxide dismutase (SOD), and peroxidase(POD)all increased, the rate of product of O2-.,and the accumulation of MDA w ere lower than those of the cont rol .Theseindicated that prechilling could improve bo th the act ive oxygen metabolism and the seedling grow th of soybeanseeds during germinat ing period .

参考文献/References:

1 　Jarvise, B .C ., Frankland, B ., C herry , Y.H .Increased DNA template and RNA polymerase associat ed wi th the breaking of seed dormancy[J] .Plant Physiol , 1968(43), 1734 -1739 .

2 　傅爱根, 王爱国, 罗广华.大豆萌发过程中的活性氧代谢[ J] .热带与亚热带植物学报, 1997 , 5(4):32 -38 .
3 　Dhindsa R S .Drought stress , enzymes of glutathione metabolism , oxidation injury ,and prot ein synthesis in tortulia turalis[ J] .Plant Physical,1991 , 95 :648 .
4　顾龚平, 吴国荣, 陆长梅, 等.PEG 处理对大豆幼苗活力及活性氧代谢的影响[ J] .中国油料作物学报, 2000 , 22(4):26 -30 .
5　陈光仪, 傅加瑞.PEG 处理种子的条件及模拟[ J] .种子, 1983(3):16 -20 .
6 　张志良.植物生理学实验指导(第二版)[ M] .北京:高等教育出版社, 1990 .133 -135 .
7　Bradf ord M M .A rapid and sensi tive method for the quanti tation ofmicrogram quantit ies of protein synthesis in t ortulia turalis [ J] .PlantPhysical , 1991 , 95 :648 .
8　王爱国, 罗广华.植物的超氧自由基与羟胺反应的定量关系[ J] .植物生理学通讯, 1990(6):55 -57 .
9 　Heath RL ,Parker L .Photoperit at ion in isolated chloroplasts kineticsand st oichiomet ry of f alty acid peroxidat ion [ J] .Arch Biophys .1968 , 25 :189 -198 .
10 　S tew ert R C , Bew ley J D .Lipid peroxidation associat ed with acceleratedaging of soybean axes [ J] .Phant physiol , 1980 , 65 :245 -248 .
11　李瑞智, 黄林.SO2 对作物叶片过氧化物酶的影响[ J] .西南师范大学学报, 1984(3):380 -385 .
12 　Rich P R, Bonner WD.The si tes of superoxide anion generat ion inhigher plant mit ochondria [ J] .Biochem Biophys , 1978 , 188 :20 -213 .
13　Koller , D.S eed Biology[ M] .Kozlowski , T .T(ed)Academic PressNew York and London .1972 , PP.2 ～ 101 .
14 　Dhindsa RS , Dhindsa PP, Reid DM .Leaf senescence and lipid peroxidation:Eff ects of some phytohormones and scavengers of f ree radicalsand singlet oxygen[ J] .Plant Physiol 1982 , 56 :453 -457 .
15 　吴国荣, 陆长梅, 陶明煊, 等.百草枯和H2O2 预处理提高盐泽螺旋藻对铅的耐受性[ J] .湖泊科学, 2000 , 12(3):240-246 .

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备注/Memo

基金项目:江苏省科委技术委员会自然基金项目:(1998SWXOSZ001)

作者简介:宰学明(1968 -), 男, 硕士研究生, 主要从事植物生理生化方面的研究。

更新日期/Last Update: 2016-12-29