[1]张宇航,李永光,王雪松,等.大豆GmGLP10 基因的克隆及生物信息学分析[J].大豆科学,2016,35(03):388-393.[doi:10.11861/j.issn.1000-9841.2016.03.0388]
　ZHANG Yu-hang,LI Yong-guang,WANG Xue-song,et al.Cloning and Bioinformatics Analysis of GmGLP10 in Soybean[J].Soybean Science,2016,35(03):388-393.[doi:10.11861/j.issn.1000-9841.2016.03.0388]
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大豆GmGLP10 基因的克隆及生物信息学分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第35卷 期数: 2016年03期 页码: 388-393 栏目: 出版日期: 2016-05-20
Title:
Cloning and Bioinformatics Analysis of GmGLP10 in Soybean
作者:
张宇航; 李永光; 王雪松; 孙铭阳; 勾涵; 陈薇; 李悦; 李文滨
东北农业大学农学院大豆生物学教育部重点实验室/农业部东北大豆生物学与遗传育种重点实验室，黑龙江哈尔滨150030
Author(s):
ZHANG Yu-hang; LI Yong-guang; WANG Xue-song; SUN Ming-yang; GOU Han; CHEN Wei; LI Yue; LI Wen-bin
Agronomy College of Northeast Agricultural University /Key Laboratory of Soybean Biology in Chinese Ministry of Education /Key Laboratory of SoybeanBiology and Genetics Breeding，Chinese Agriculture Ministry，Harbin 150030，China)ion as an Auxin binding protein to regulate growth and development of soybeanand disease resistance defense responses.
关键词:
大豆;  GmGLP10 基因;  克隆;  菌核病;  生长素结合蛋白
Keywords:
Soybean;  GmGLP10 gene;  Cloning;  Sclerotinia Sclerotiorum;  Auxin binding protein
分类号:
S565. 1
DOI:
10.11861/j.issn.1000-9841.2016.03.0388
文献标志码:
A
摘要:
通过PCＲ 的方法从大豆抗菌核病品种Maple Arrow 中克隆得到GmGLP10 基因，生物信息学分析结果显示GmGLP10 蛋白由213 个氨基酸组成，具有一个糖基化位点和多个磷酸化位点，为胞外分泌蛋白。通过对GmGLP10 基因起始密码子上游1 500 bp 序列进行顺式作用元件分析，预测GmGLP10 启动子上具有多个与激素和防御胁迫应答相关的顺式作用元件。进化树分析结果表明，GmGLP10 与多个生长素结合蛋白进化距离较近，推断其可能具有相似的功能。GmGLP10 基因在菌核病菌胁迫下的转录本丰度的变化结果表明在菌核胁迫处理后GmGLP10 基因表达量上调明显。GmGLP10 可能作为生长素结合蛋白参与调控大豆的生长发育与抗病防御应答反应。
Abstract:
GmGLP10 gene was isolated from soybean Sclerotinia Sclerotiorum resistant cultivar Maple Arrow by PCＲ method.Bioinformaties analysis showed that GmGLP10 gene encoding a 213 amino acids protein，with a N-glycosylation site and severalphosphorylation sites and was putatively to be a extracellular protein. The Upstream 1 500 bp sequence of GmGLP10 genewere analyzed，multiple cis-elements related to hormone and defense stress responses existed in the GmGLP10 promoter region.Phylogenetic analysis results indicted that GmGLP10 and several Auxin binding proteins( ABPs) had closer genetic relationship，that may have similar functions. The GmGLP10 transcript abundance was significantly increased after Sclerotia infection.These results suggested GmGLP10 may funct

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备注/Memo

基金项目: 抗逆转基因大豆新品种培育( 2015ZX08004002) ; 东北农业大学“青年才俊”项目( 14QC26) 。

第一作者简介: 张宇航( 1991-) ，男，博士，主要从事大豆遗传育种研究。E-mail: yuhangzhangneau@163. com。
通讯作者: 李文滨( 1958-) ，男，教授，博导，主要从事大豆遗传育种研究。E-mail: wenbinli@ neau. edu. cn。

更新日期/Last Update: 2016-06-28