[1]井妍,孙晶,高赛男,等.大豆GmST1 基因的生物信息学分析[J].大豆科学,2016,35(03):394-401.[doi:10.11861/j.issn.1000-9841.2016.03.0394]
　JING Yan,SUN Jing,GAO Sai-nan,et al.Bioinformatics Analysis of ST1 Gene in Soybean[J].Soybean Science,2016,35(03):394-401.[doi:10.11861/j.issn.1000-9841.2016.03.0394]
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大豆GmST1 基因的生物信息学分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第35卷 期数: 2016年03期 页码: 394-401 栏目: 出版日期: 2016-05-20
Title:
Bioinformatics Analysis of ST1 Gene in Soybean
作者:
井妍; 孙晶; 高赛男; 赵雪; 滕卫丽; 韩英鹏; 李文滨
东北农业大学农学院/大豆生物学教育部重点实验室/东北农业大学农业部东北大豆生物学与遗传育种重点实验室，黑龙江哈尔滨150030
Author(s):
JING Yan; SUN Jing; GAO Sai-nan; ZHAO Xue; TENG Wei-li; HAN Ying-peng; LI Wen-bin
Key Laboratory of Soybean Biology in Chinese Ministry of Education /Key Laboratory of Soybean Biology and Breeding ( Genetics) of Chinese AgricultureMinistry，Agronomy College of Northeast Agricultural University，Harbin 150030，China
关键词:
大豆;  GmST1;  磺基转移酶;  蛋白质结构分析;  功能预测
Keywords:
Soybean;  GmST1;  SULT;  Protein structure analysis;  Functional prediction
分类号:
S565. 1
DOI:
10.11861/j.issn.1000-9841.2016.03.0394
文献标志码:
A
摘要:
磺基转移酶( sulfotransferase，SULT ) 基因是一个基因超家族。对大豆磺基转移酶基因GmST1( Glyma. 13G191400) 进行基于生物信息学的基因结构分析与功能预测，结果表明: GmST1( Williams82) 基因序列全长1 439 bp，CDS 区长1 035 bp，编码344 个氨基酸。通过序列比对，分析出在感病Williams 82 和抗病品种东农93-046中，GmST1 序列存在着非同义SNP，导致氨基酸改变。利用PlantCAＲE 分析启动子元件，发现在基因启动子序列中含有多个与光诱导、生长素、水杨酸、干旱等相关的元件。在BAＲ 数据库中分析了GmST1 的拟南芥同源基因在不同逆境胁迫条件下基因表达情况，表明该基因表达具有组织表达特异性，参与盐胁迫、干旱胁迫和抗病等相关反应。
Abstract:
Sulfotransferase( SULT) is a gene superfamily. In this study，we mainly analyzed the structure and function of ST1gene in soybean based on bioinformatics. The results showed that the full sequence length of GmST1( Williams 82) was 1 439bp. The CDS was 1 035 bp in length， encoding 344 amino acids. By sequence alignment， one non-synonymous SNP was foundbetween Williams 82 and Dongnong 93-046， leading to the changes of amino acid. There were multiple cis-elements about lightresponses，SA，drought and auxin in promoter sequence region detected by PlantCAＲE. The Bio-Analytic Ｒesource for PlantBiology database was used to analyze the different expression of the homologous gene of GmST1 in Arabidopsis. The results indicatedthat the gene had tissue-special expression and was involved in salt，drought and diseases responses.

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备注/Memo

基金项目: 国家现代农业产业技术体系( CAＲS-04-PS04) ; 国家自然科学基金( 31301339) ; 黑龙江省教育厅科学技术研究项目( 12541049) ;黑龙江省普通高等学校新世纪优秀人才培养计划( 1253-NCET-005) 。

第一作者简介: 井妍( 1991-) ，女，硕士，主要从事大豆遗传育种研究。E-mail: 1497678645@ qq. com。
通讯作者: 李文滨( 1958-) ，男，教授，博导，主要从事大豆遗传育种研究。E-mail: wenbinli@ neau. edu. cn;韩英鹏( 1978-) ，男，教授，博导，主要从事大豆遗传育种和分子生物技术研究。E-mail: hyp234286@ aliyun. com。

更新日期/Last Update: 2016-06-28