[1]魏崃,张丽,王伟威,等.大豆病程相关蛋白PＲ-5 及其同源蛋白TPLs 的生物信息学分析[J].大豆科学,2016,35(03):380-387.[doi:10.11861/j.issn.1000-9841.2016.03.0380]
　WEI Lai,ZHANG Li,WANG Wei-wei,et al.Bioinformatics Analysis on Soybean Pathogenesis-related Protein( PＲ-5) and ItsHomology Proteins TPLs[J].Soybean Science,2016,35(03):380-387.[doi:10.11861/j.issn.1000-9841.2016.03.0380]
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大豆病程相关蛋白PＲ-5 及其同源蛋白TPLs 的生物信息学分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第35卷 期数: 2016年03期 页码: 380-387 栏目: 出版日期: 2016-05-20
Title:
Bioinformatics Analysis on Soybean Pathogenesis-related Protein( PＲ-5) and ItsHomology Proteins TPLs
作者:
魏崃1; 2; 张丽1; 王伟威1; 于志远1; 喻德跃2; 刘丽君1
1. 黑龙江省农业科院大豆研究所，黑龙江哈尔滨150086;?
2. 南京农业大学农学院，江苏南京210095
Author(s):
WEI Lai1; 2; ZHANG Li1; WANG Wei-wei1; YU Zhi-yuan1; YU De-yue2; LIU Li-jun1
1. Soybean Ｒesearch Institute，Heilongjiang Academy of Agricultural Sciences，Harbin 150086，China;?
2. Agronomy College of Nanjing ArgriculturalUniversity，Nanjing 210095，China
关键词:
大豆病程相关蛋白;  类甜蛋白;  系统进化分析;  生物信息学分析
Keywords:
Soybean pathogenesis-related proteins;  TLPs;  Evaluation analysis;  Bioinformatics
分类号:
S565. 1
DOI:
10.11861/j.issn.1000-9841.2016.03.0380
文献标志码:
A
摘要:
?大豆病程相关蛋白PＲ-5 又称为类甜蛋白TLPs，广泛分布于高等植物体内，各种生物和非生物胁迫诱导( 病原微生物、渗透胁迫，机械损伤和植物激素等) 均可诱导其表达。本研究以PＲ-5 编码基因Glyma01g42670. 1 为目的基因，对该基因及其同源蛋白的结构和功能进行了分析; 通过生物信息学的方法，共筛选获得42 条大豆TLPs 蛋白质序列，并用Mega 4. 1 软件构建其系统进化树; 同时对SoyBase 中的大豆TLPs 相关的表达数据进行分析。结果表明: 大豆病程相关蛋白PＲ-5 的编码基因Glyma01g42670. 1 编码240 个氨基酸的多肽链，pI 6. 26，是一种酸性蛋白，属于疏水蛋白，该蛋白质具有典型的TLP 家族的保守结构域，具有指导PＲ-5 蛋白的跨膜转移( 定位) 的N 端信号肽序列，PＲ-5 蛋白不含有跨膜螺旋结构，主要存在于质外体中，属于分泌蛋白，推测该蛋白具有内切葡聚糖酶的催化活性，进化分析表明: 该编码基因与拟南芥的NP_192902. 1 基因属于直系同源基因，它们是具有相同功能和共同起源的基因，组织表达特异性分析表明该基因主要在根部和花中表达。
Abstract:
Protein PＲ-5 of soybean is one of the PＲs，known as TLPs，which exist in higher plants and can be induced to expressby biotic and abiotic stress( including pathogenic microorganisms，osmotic stress，mechanical damage and phytohormoneetc. ) . In present study，the structure and function of gene Glyma01g42670. 1 and its homologous protein were analyzedthrough bioinformatics，meanwhile 42 soybean TLPs protein sequences were collected to build a phylogenetic tree by softwareMega 4. 1，and all the expression data of TLPs from website SoyBase were analyzed. The results showed: Gene Glyma01g42670.1 coded peptide with 240 amino acids，and the protein was acidic and hydrophobic with pI 6. 26，which had typicaldomain as TLP family. It had N-terminal signal peptide leading protein membrane transportation，but without transmembranehelix structure，and mainly existed in apoplast. It belonged to secreted proteins and inferred to have endoglucanase catalyticactivity. From the results of evaluation analysis，gene Glyma01g42670. 1 was the orthologous gene of gene NP_192902. 1from Arabidopsis，which had the same function and the same origin. From the results of tissue differential analysis，this genemainly expressed in roots and flowers. All the research helps to clarify the function and molecular mechanism of how TLPsgene family did during the plant development.

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备注/Memo

基金项目: 国家现代农业产业技术体系( CAＲS-04-PS05) ; 哈尔滨市应用技术与开发项目( 2013ＲFQYJ016) ; 黑龙江省农业科技创新工程( 2014ZD011) 。

第一作者简介: 魏崃( 1985-) ，女，博士，助理研究员，主要从事大豆分子育种研究。E-mail: adalwei@126. com。
通讯作者: 刘丽君( 1958-) ，女，研究员，主要从事大豆遗传育种研究。E-mail: nkyssbd@126. com。

更新日期/Last Update: 2016-06-23