WU Guang-xi,LIU Li-jun,YANG De-guang,et al.Heat Resistance Evaluation of GmHSFA1 Transgenic Soybean[J].Soybean Science,2012,31(03):341-346.[doi:10.3969/j.issn.1000-9841.2012.03.002]
转GmHSFA1基因大豆的耐热性评价
- Title:
- Heat Resistance Evaluation of GmHSFA1 Transgenic Soybean
- 文章编号:
- 1000-9841(2012)03-0341-06
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 在高温胁迫条件下对11个转GmHSFA1基因的大豆品系及其受体的GmHSFA1及靶基因GmHSP70、GmHSP22和GmHSP17.9的表达和生理生化、光合特性及产量性状变化进行了研究,并采用胁迫系数与灰色关联分析相结合方法,对转基因大豆材料耐高温能力进行综合评价。结果表明:多数转基因大豆品系在常温和热诱导条件下GmHSFA1及靶基因GmHSP70、GmHSP22和GmHSP17.9表达水平明显高于非转基因受体;高温处理后,绝大多数转基因材料的脯氨酸含量增幅都要明显高于非转基因受体,丙二醛含量除HTH-4以外增幅均小于非转基因受体,可溶性糖含量增幅全部高于非转基因受体材料。多数转基因材料的光合性状与产量性状降幅均小于非转基因受体。关联度分析表明与非转基因受体相比,有9个转GmHSFA1基因品系的耐热能力得到明显提高。
- Abstract:
- In this study,the expression of GmHSFA1,GmHSP70,GmHSP22 and GmHSP17.9 in 11 GmHSFA1 transgenic soybean lines and their receptor were evaluated and researched the change in physiological indices(Pro,MDA,Wss),photosynthetic characteristics(Pn,Cond,Ci,Tr)and yield after high temperature treatment.In most of the transgenic soybean lines,the expression of GmHSFA1、GmHSP70、GmHSP22 and GmHSP17.9 were higher than non-transgenic receptor at 28℃ and 48℃.After heat treatment,the increment of proline content and soluble sugar in most transgenic soybean lines were clearly higher than non-transgenic receptor,increment of MDA content were lower than receptor except HTH-4,the decrement of photosynthetic traits and yield in most of the transgenetic materials were less than non-transgenic receptor.Through gray relation analysis,we concluded that the heat resistance of 9 GmHSFA1 transgenic lines were improved significantly than non-transgenic receptor.
参考文献/References:
[1]陈晓军,叶春江,吕慧颖,等.GmHSFA1克隆及其过量表达提高转基因大豆的耐热性[J].遗传,2006,28(11):1411-1420.(Chen X J,Ye C J,LüH Y,et al.Cloning of GmHSFA1gene and its overexpression leading to enhancement of heat tolerance in transgenic soybean[J].Hereditas,2006,28(11):1411-1420.) [2] Morimoto R I.Regulation of the heat stress transcriptional response:cross talk between family of heat stress factors,molecular chaperones,and negative regulators[J].Genes& Development,1998,12(24):3788-3796. [3] Nakai A.New aspects in the vertebrate heat stress factor system:HSFA3 and HSFA4[J].Cell Stress and Chaperones,1999,4:86-93. [4] Nover L,Bharti K,Dring P,et al.Arabidopsisand the heat stress transcription factor world:How many heat stress transcription factors do we need?[J].Cell Stress and Chaperones,2001,6(3):177-189. [5] Schffl F,Prndl R,Reindl A.Regulation of theheat-shock response[J].Plant Physiologists,1998,117(4):1135-1141. [6] Wu C.Heat shock transcription factors:structure and regulation[J].Annual Review of Cell Developmental Biology,1995,11:441-469. [7] 李春光,陈其军,高新起,等.拟南芥热激转录因子AtHSFA2调节胁迫反应基因的表达并提高热和氧化胁迫耐性[J].中国科学(C:生命科学),2005,35(5):398-407.(Li C G,Chen Q J,Gao X Q,et al.The role of heat shock transcription factor AtHSFA2on heat and oxidative stress response in Arabidopsis[J].Science in China(Series C:Life Sciences),2005,35(5):398-407.) [8] Charng Y Y,Liu H C,Liu N Y,et al.A heat-inducible transcription factor,HSFA2,is required for extension of acquired thermotolerance in Arabidopsis[J].Plant Physiology,2007,143(1):251-262. [9] Li C,Chen Q,Gao X,et al.AtHSFA2modulates expression of stress responsive genes and enhances tolerance to heat and oxidative stress in Arabidopsis[J].China Science:Life Sciences in English,2005,48(6):540-550. [10]邵玲,陈向荣.热激蛋白与植物的抗逆性[J].北方园艺,2005(3):73-74.(Shao L,Chen X R.Study of the heat shock protein and the plant stress resistance physiology[J].Northern Horticulture,2005(3):73-74.) [11]唐晓飞,刘丽君,高明杰,等.农杆菌介导热激转录因子8基因转化大豆[J].分子植物育种,2009,7(3):444-450.(Tang X F,Liu L J,Gao M J,et al.Agrobacterium-mediated transformation of hsf8 into soybean[J].Molecular Plant Breeding,2009,7(3):444-450.) [12]王晶英,敖红,张杰,等.植物生理生化实验技术与原理[M].哈尔滨:东北林业大学出版社,2003:133-136.(Wang J Y,Ao H,Zhang J,et al.Plant physiological and biochemical technologyand principle[M].Harbin:Northeast Forestry University Press,2003:133-136.) [13]杨柳,张振乾,宋继金,等.植物抗逆基因研究进展[J].作物研究,2010,24(2):126-129.(Liu Y,Zhen Q Z,Song J J,et al.Research progress of plant stress-resistant genes[J].Crop Research,2010,24(2):126-129.) [14]翁锦周,洪月云.植物热激转录因子在非生物逆境中的作用[J].分子植物育种,2006,4(1):88-94.(Weng J Z,Hong Y Y.The roles of plant shock transcription factors in abiotic stress[J].Molecular Plant Breeding,2006,4(1):88-94.) [15]张伟.热休克基因转录的调节:热休克转录因子(HSF)的结构与功能[J].第三军医大学学报,2000,22(2):198-200.(Zhang W.Regulation of heat shock gene transcription:the structure and function of heat shock transcription factor[J].Acta Academiae Medicinae Militari Tertiae,2000,22(2):198-200.) [16]Zhu B G,Ye C J, Lü H Y,et al.Identification and characterization of a novel heat shock transcription factor gene,GmHsfA1,in soybeans(Glycine max)[J].Journal of Plant Research,2006,119:247-256.
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备注/Memo
基金项目:国家转基因重大专项资助项目(2008ZX08004-002);大豆产业技术体系资助项目(CARS04-ps05)。