[1]张原宇,王玉民,于志晶,等.叶酸代谢途径folE基因表达载体的构建及在大豆中的转化[J].大豆科学,2015,34(02):224-227.[doi:10.11861/j.issn.1000-9841.2015.02.0224]
　ZHANG Yuan-yu,WANG Yu-min,YU Zhi-jing,et al.Construction of the Folate Biosynthetic Pathway folE Gene and Transformation in Soybean[J].Soybean Science,2015,34(02):224-227.[doi:10.11861/j.issn.1000-9841.2015.02.0224]
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叶酸代谢途径folE基因表达载体的构建及在大豆中的转化

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第34卷 期数: 2015年02期 页码: 224-227 栏目: 出版日期: 2015-04-25
Title:
Construction of the Folate Biosynthetic Pathway folE Gene and Transformation in Soybean
作者:
张原宇; 王玉民; 于志晶; 李海云; 张玲
吉林省农业科学院农业生物技术研究所，吉林 长春 130033
Author(s):
ZHANG Yuan-yu;  WANG Yu-min;  YU Zhi-jing;  LI Hai-yun;  ZHANG Ling
AgroBiotechnology Research Institute, Jilin Academy of Agricultural Sciences, Changchun 130033, China
关键词:
大豆; 叶酸; folE; 转基因
Keywords:
Soybean;  Folate;  folE;  Transgenic
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2015.02.0224
文献标志码:
A
摘要:
folE基因是高叶酸调控基因，来源于细菌，已证明在西红柿等植物中，提高了叶酸含量。本试验采用农杆菌介导的大豆子叶节法，将folE基因在大豆受体材料Williams82中表达。共侵染了200个外植体，得到34株转化苗。通过对转化苗进行除草剂（135 mg?L-1）涂抹鉴定，获得14株除草剂抗性转化苗。PCR检测结果显示其中12株为阳性。Southern杂交结果表明，folE全长cDNA已成功插入到大豆基因组中，并得到2个单拷贝株系。
Abstract:
The folE is a key gene of folic acid pathway, which is derived from bacteria.The function of this gene in the improved folate content has been demonstrated in many plants，such as tomato In this study, we expressed the -folE-by employing the soybean cotyledonary nodes method.We co-cultivated about 200 explants, and got 34 transgenic plants. Stock of Basta (135 mg?L-1) was painted on half the upper surface of tested soybean leaves, and got 14 transgenic plants. PCR analysis showed that 12 of 14 transgenic plants were positive Southern blot analysis of PCRpositive plants indicated the integration of the-folE- gene into the soybean genome, and we also got two transgenic lines with only one single copy.

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备注/Memo

基金项目：国家高技术研究发展计划“863计划”（2012AA101106-3）；吉林省2012年度博士后科研项目（20120010407）；转基因生物新品种培育重大专项（2013ZX08004）。

第一作者简介：张原宇（1983-），男，硕士，主要从事大豆转基因生物技术。E-mail：zyy_1983@foxmail.com。
通讯作者：张玲（1981-），女，博士，助理研究员，主要从事植物分子生物学研究。E-mail：zly_jaas@126.com。

更新日期/Last Update: 2015-06-07