[1]李海芬,刘庆梅,杨光宇,等.硼酸诱导的大豆子叶节转化新方法[J].大豆科学,2011,30(02):194-197,204.[doi:10.11861/j.issn.1000-9841.2011.02.0194]
　LI Hai-fen,LIU Qing-mei,YNAG Guang-yu,et al.Boric Acid Mediated Transformation of Soybean (Glycine max) Cotyledonary Node[J].Soybean Science,2011,30(02):194-197,204.[doi:10.11861/j.issn.1000-9841.2011.02.0194]
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硼酸诱导的大豆子叶节转化新方法

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第30卷 期数: 2011年02期 页码: 194-197,204 栏目: 出版日期: 2011-04-25
Title:
Boric Acid Mediated Transformation of Soybean (Glycine max) Cotyledonary Node
文章编号:
1000-9841（2011）02-0194-04
作者:
李海芬1; 刘庆梅1; 杨光宇2; 王洋2; 岳娜1; 胡倩倩1; 孟祥勋1
1.苏州大学 基础医学与生物科学学院，江苏 苏州 215123；
2.吉林省农业科学院 大豆研究中心，吉林 长春 130033
Author(s):
LI Hai-fen1; LIU Qing-mei1; YNAG Guang-yu2; WANG Yang2; YUE Na1; HU Qian-qian1; MENG Xiang-xun1
1.School of Basic Medicine and Biological Science, Soochow University, Suzhou 215123，Jiangsu;?
2.Soybean Insititute, Jilin Academy of Agricultural Sciences, Changchun 130033，Jilin, China
关键词:
大豆; 转基因; 农杆菌; 硼酸; 丛生芽
Keywords:
Soybean; Transgene; Agrobacterium; Boron acid; Multiple shoots
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2011.02.0194
文献标志码:
A
摘要:
采用农杆菌介导法，以苏云金芽孢杆菌(Bacillus thuringiensis)毒性蛋白基因Bt为目的基因，研究了不同硼酸浓度处理对大豆子叶节转基因的影响。结果表明：200 mg·L-1硼酸浓度诱导培养基诱导的大豆丛生芽长势最好，而且转基因效率高达3.3%。对获得大豆转化幼苗的根和叶片采用PCR、逆转录PCR和Southern blotting等方法进行筛选与鉴定，结果均有2株幼苗呈阳性。
Abstract:
The agrobacterium-mediated transgenic technique combined with the treatment of boric acid was studied for the transformation of foreign gene Bt to soybean cotyledon nodes. The results showed that shoots regenerated from the explants were multiplied best at 200 mg·L-1 of boric acid concentration, and the transformation ratio reached 3.3%. Regenerated seedlings were screened by genomic PCR amplification and two seedlings on inductive medium of 200 mg·L-1 boron acid was obtained and proved to be positive by reverse PCR and Southern blotting. It was demonstrated that the target gene had been transformed into the soybean genome.

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备注/Memo

基金项目：吉林省科技发展计划重点资助项目（20060202）。

第一作者简介：李海芬（1984-），女，硕士，研究方向为植物基因与分子遗传学。E-mail:lihaifen.555@163.com。
通讯作者：孟祥勋（1955-），男，教授，博士，从事植物分子遗传与基因工程研究。E-mail:mengxiangxun@suda.edu.cn。

更新日期/Last Update: 2014-09-11