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微信公众号：大豆科学

[1]雷勃钧单红吕晓波朱水方陈红运赵文军.PCR -ELISA 法对大豆品种的转基因定性检测研究[J].大豆科学,2004,23(01):55-58.[doi:10.11861/j.issn.1000-9841.2004.01.0055]
　Lei BojunLu XiaoboShan HongZhu ShuifangChen Hongy unZhao Wenjun.STUDYON QUALITATIVE TESTOFTRANSGENES BY PCR-ELISA IN SOYBEAN CULTIVARS[J].Soybean Science,2004,23(01):55-58.[doi:10.11861/j.issn.1000-9841.2004.01.0055]

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PCR -ELISA 法对大豆品种的转基因定性检测研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第23卷期数: 2004年01期页码: 55-58 栏目: 出版日期: 2004-02-25

Title:: STUDYON QUALITATIVE TESTOFTRANSGENES BY PCR-ELISA IN SOYBEAN CULTIVARS

文章编号:: 1000 -9841(2004)01 -0055 -04

作者:: 雷勃钧¹单红¹吕晓波¹朱水方²陈红运²赵文军²; 1.黑龙江省农业科学院, 哈尔滨150086 ;
2.国家出入境检验检疫局动植物检疫实验所, 北京100029

Author(s):: Lei Bojun¹Lu Xiaobo¹Shan Hong¹Zhu Shuifang²Chen Hongy un²Zhao Wenjun²; 1.Heilongj iang Academy of Agricultural Sciences , Harbin 150086 ;
2.Quarantine Institute in Animals and Plants of S tate Quality Test Bureau , Beijing 100029

关键词:: 大豆; 转基因; 外源DNA 直接导入; 定性检测

Keywords:: Soybean ; T ransgene ; Di rect int roduction exogenous DNA ; Quali tative analy sis

分类号:: S 565.1

DOI:: 10.11861/j.issn.1000-9841.2004.01.0055

文献标志码:: A

摘要:: 以共价交联在PCR 管壁上的寡核苷酸作为固相引物进行PCR 扩增, 对PCR 扩增的固相和液相产物分别进行杂交和凝胶电泳检测的PCR -ELISA 法, 对黑龙江省常规选育品种合丰35 和黑农37 、外源DNA 直接导入大豆的分子育种所获品种黑生101 、及大连进口的美国2 号等大豆, 进行转基因定性检测。结果表明:该方法高效可靠, 可作为一种快速定性检测转基因产品的方法;检测结果:美国2 号为转基因大豆, 黑生101 、合丰35 和黑农37 为非转基因大豆。上述结果对分子育种、生态保护、安全监测及对建立黑龙江省非转基因大豆生产保护区等具有重要意义。

Abstract:: The oliqonucletides w hich covalent primers , bonded on the w all of a PCR tube as solid primers , havebean amplified by PCR equipment .The Solid and Liquid phase amplified by PCR were hy bridized and done w ithgel -eleitro phoresis respectively .Some Heilongjiang soybean cultivars, Suchas Hefeng 35 and Heinong 37(bredby conventional breeding), Heisheng 101(bred by introducing exogenous DNA through pollen tube channel , andSoybean of American 2 from Dalian importing , were tested by the PCR -ELISA .The result showed that Soybeanof American 2 w as a transgenic soy bean , Heisheng 101 , Hefeng 35 and Heinong 37 w ere no t .Those haveimportant meaning in plant molecular breeding , ecological pro tecting and safety monito ring .

参考文献/References:

1 　赵文军, 陈红运, 黄文胜.PCR -ELISA 在转基因产品检测中的应用[ J] .植物检疫, 2001 , 15(5):297-299.

2 　姚文国, 朱水芳主编.国外转基因产品检测技术汇编[ J] .深圳:深圳出入镜检验检疫局, 2001 :P1 -7 , 38 -45.

3 　王国英.转基因植物的安全性评价[ J] .农业生物技术学报,2001 , 9(3):205-207.

4 　S .F .Zhu ,.2000.Est ablishent of Aunque Method for Rapid Det ectionof GMOs product s :C apture PCR-ELISA In the proceeding ofthe First Asian phyt opathogy congress 2000 , 8 :12-17.

5 　Markus L , Peter B .I UPAC collaborative t rial study of a merhod t odet cor genct ically mcdif ied soybcans and msize in dried pow der J .AOAC Intcr .1999 , 84(4):923-928.

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备注/Memo

作者简介:雷勃钧(1944 -), 女, 研究员, 研究方向农业生物技术。

更新日期/Last Update: 2016-12-31