CHENG Ying-xin,LIANG Ji-li,LIU Qing-li,et al.Changes of Total Polyphenol Content in Soybean Treated by Pathotoxin Produced by Phytophthora sojae[J].Soybean Science,2014,33(04):524-527.[doi:10.11861/j.issn.1000-9841.2014.04.0524]
疫霉根腐病菌毒素对大豆不同组织中总多酚含量的影响
- Title:
- Changes of Total Polyphenol Content in Soybean Treated by Pathotoxin Produced by Phytophthora sojae
- Keywords:
- Soybean; Phytophthora sojae; Pathotoxin; Total polyphenol
- 分类号:
- 565.1
- 文献标志码:
- A
- 摘要:
- 对大豆疫霉根腐病菌毒素胁迫下抗感不同大豆品种根、茎、叶中总多酚的含量进行了初步研究。结果表明:适宜浓度的毒素(稀释100倍,浓度为0.089 7 mg?mL-1)处理后,抗病品种根、茎和叶中总多酚在病程的大部分阶段与对照相比升高;而感病品种在整个病程中虽然在某些阶段较对照有一定的提高,但幅度不大,在病程其他阶段总多酚下降幅度远大于升高幅度。说明适宜浓度的大豆疫霉菌毒素可以起到激发子作用。
- Abstract:
- In this study,changes of total polyphenol content of nine soybean cultivars with different resistance treated by the pathotoxin of Phytophthora sojae was investigated.The results showed that the total polyphenol content in roots,stems,and leaves of resistant soybean cultivars treated by the pathotoxin with optimal concentration(Diluted to 100 times and the concentration was 0.089 7 mg?mL-1)was higher than that of the control at most of the pathogenic stages.The total polyphenol content in susceptible soybean cultivars increased compared with that in the control at some of the pathogenic stages,but decreased at most of the pathogenic stages.It shows that appropriate concentration of pathtoxin prouduced from P.sojae may play an important role as an elietior in resistance to pathogen infection.
参考文献/References:
[1]Schmitthenner A F.Problems and progress in control of phytophthora root rot of soybean[J].Plant Disease,1985,69(4):362-368.
[2] Anderson T R,Tenuta A.Phytophthora rot[M]//Bailey K L,Gossen B D,Gugel R K,et al.Diseases of field crops in Canada.Saskatoon:Canadian Phytopathological Society,University Extension Press University of Saskatchewan,2003:155-156. [3] Zhang S Z,Xu P F,Wu J J,et al.Races of Phytophthora sojaeand their virulences on soybean cultivars in Heilongjiang,China[J].Plant Disease,2010,94(1):87-91. [4] 张淑珍,徐鹏飞,吴俊江,等.大豆疫霉根腐病菌毒素处理抗感不同大豆品种后苯丙氨酸解氨酶活性的变化[J].作物杂志,2008(1):47-49.(Zhang S Z,Xu P F,Wu J J,et al.Chang of phenylalanine Ammonia-lyase(PAL)activities among different resistance soybean varieties treated with pathotoxin from Phytophthora soja[J].Crops,2008(1):47-49.) [5] Tsuge T,Nishimura S,Kobayashi H.Metabolic regulation of host-specific toxin production in Alternaria alternatapathogens(4)molecular cloning of mRNA in AK-toxin producing isolate[J].Annals of the Phytopathological Society of Japan,1986,52:690-699. [6] 郭霞.红豆草黑腐病菌毒素的研究[D].兰州:甘肃农业大学,2009:4-18.(Guo X.Study on the toxin production by black rot of sainfoin[D].Lanzhou:Gansu Agricultural University,2009:4-18.) [7] 章元寿.植物病原真菌毒素的研究现状[J].真菌学报,1991,10(3):169-181.(Zhang Y S.Research status of the toxins produced by plant pathogenic fungi[J].Acta Mycologica Sinica,1991,10(3):169-181.) [8] 祁高富,杨斌,叶建仁.植物病原真菌毒素研究进展[J].南京林业大学学报,2000,24(2):66-70.(Qi G f,Yang B,Ye J R.Research advances on the toxin of the plant pathogenic fungus[J].Journal of Nanjing Forestry University,2000,24(2):66-70.) [9] 罗孟军,朱天辉.植物病原真菌毒素[J].四川林业科技,2001,22(3):45-49.(Luo M J,Zhu T H.Phytotoxin in plant pathogen[J].Journal of Sichuan Forestry Science and Technology,2001,22(3):45-49.) [10]张淑珍,徐鹏飞,武小霞,等.大豆疫霉根腐病毒素的提取及生物活性的测定[J].中国农学通报,2005,21(3):252-254.(Zhang S Z,Xu P F,Wu X X,et al.Study on extraction and bioactivity assay pathotoxin produced by Phytophthora sojae[J].Chinese Agricultural Science Bulletin,2005,21(3):252-254.) [11]Bhattacharyya M K,Ward E W B.Biosynthesis and metabolism of glyceollin I in soybean hypocotyls following wounding or inoculation with phytophthora megaspermaf.sp.glycinea[J].Physiological and Molecular Plant Pathology,1987,31:387-405. [12]Ebel J,Grisebach H.Defense strategies of soybean against the fungus Phytophthora megaspermf.sp.glycinea:A molecular analysis[J].Trends Biochemical Science,1988,13:23-27. [13]王欣,靳丽梅,徐鹏飞,等.野生大豆接种大豆疫霉根腐病菌后总多酚含量的变化[J].作物杂志,2012(4):78-82.(Wang X,Jin L M,Xu P F,et al.Response of total polyphenol activity in wild soybean inoculated by Phytophthora sojae[J].Crops,2012(4):78-82.) [14]Harbone J B.The comparative biochemistry of phytoalexin induction in plant[J].Biochemical Systematica and Ecology,1999,27:335-368. [15]陈兵,王坤元,董国强,等.水稻纹枯病菌致病性与酶活力的关系[J].浙江农业学报,1992,4(1):8-14.(Chen B,Wang K Y,Dong G Q,et al.The correlations in the pathogenicity to the enzymes’ activities of Thanatephorus cucumeriscausing rice sheath blight disease[J].Acta Agricultural Zhejianggensis,1992,4(1):8-14.) [16]徐兆飞,刘亚光.灰斑病菌对大豆叶片总多酚和总黄酮的诱导研究[J].大豆科学,2006,25(3):234-236.(Xu Z F,Liu Y G.Study on induction of total podyphenol and total flavonoid in soybean leaves by Cercospora Sojinahara[J].Soybean Science,2006,25(3):234-236.) [17]杨辉,沈火林,朱鑫,等.防御酶活性、木质素和总酚含量与辣椒抗黄瓜花叶病毒的关系[J].中国农学通报,2006,22(5):369-373.(Yang H,Shen H L,Zhu X,et al.A study on the relationship between the resistance to CMV of hot pepper and related defendant enzyme activity,the content of lignins,and total phenols[J].Chinese Agricultural Science Bulletin,2006,22(5):369-373.) [18]Yang X B,Ruff R L,Meng X Q.Races of Phytophthora sojaein Iowa soybean fields[J].Plant Disease,1996,80:1418-1420. [19]张淑珍,吴俊江,葛秀秀,等.大豆疫霉根腐病菌产生条件的研究[J].植物病理学报,2002,32(1):89-90.(Zhang S Z,Wu J J,Ge X X,et al.Study on condition of pathotoxin produced by Phytophthora sojae[J].Acta Phytopathologica Sinica,2002,32(1):89-90.) [20]高必达,吴畏,程晖,等.麦根长蠕孢毒素对抗病和感病小麦品种叶组织超微结构的影响[J].植物病理学报,1993,23(2):107-113.(Gao B D,Wu W,Cheng H,et al.Ultrastructure of leaf tissues of resistantor susceptible wheat exposed to toxin produced by Lminthosporium sativum[J].Acta Phytopathologica Sinica,1993,32(2):107-113.) [21]张淑珍,徐鹏飞,韩英鹏,等.大豆疫霉菌毒素处理大豆品种后几丁质酶活性的变化[J].作物杂志,2006(3):17-19.(Zhang S Z,Xu P F,Han Y P,et al.The chitinase activity of soybean varieties after treated with pathotoxin produced from?Phytophthora sojae[J].Crops,2006(3):17-19.) [22]陈晨,徐鹏飞,张淑珍,等.大豆疫霉根腐病菌毒素处理不同大豆品种后过氧化物酶活性的变化[J].大豆科学,2010,29(5):840-844.(Chen C,Xu P F,Zhang S Z,et al.Changes of peroxidase in soybean treated by pathotoxin produced by Phytophthora sojae[J].Soybean Science,2010,29(5):840-844.) [23]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,1999.(Li H S.Principles and techniques of plant physiological biochemical experiment[M].Beijing:Higher Education Pres,1999.) [24]陈捷,蔺瑞明,高增贵,等.玉米弯孢叶斑病菌毒素对寄主防御酶系活性的影响及诱导抗性效应[J].植物病理学报,2002,32(1):43-48.(Chen J,Lin R M,Gao Z G,et al.The influence of Curvularia lunatatoxin on defense enzyme activities and its function in the induced resistance of corn[J].Acta Phytopathologica Sinica,2002,32(1):43-48.) [25]章元寿.植物病理生理学[M].南京:江苏科学技术出版社,1994:50.(Zhang Y S.Plant apathology physiology[M].Nanjing:Jiangsu S & T Press,1994:50.) [26]张淑珍.大豆疫霉根腐病菌毒素及其诱导抗性机制的研究[D].哈尔滨:东北农业大学,2002.(Zhang S Z.Study of pathotoxin produced by Phytophthora sojaeand induced resistant mechanism of soybean treated by it[D].Harbin:Northeast Agricultural University,2002.) [27]Graham T L,Kim J E,Graham M.Role of constitutive is of lav one conjugates in the accumulation of glyceollin in soybean infected with phytophthora megasperma[J].Molecular Plant-Microbe Interactions,1990,3(3):157-166. [28]胡莉莉.香蕉抗枯萎病生理生化基础的研究[D].儋州:华南热带农业大学,2006:18-50.(Hu L L.Studies on basic of physiological and biochemical resistance of banana to fusarium wilt[D].Danzhou:South China University of Tropical Agriculture,2006:18-50.) [29]葛秀秀.大豆抗疫霉根腐病机制的初步研究[D].哈尔滨:东北农业大学,2001:45-47.(Ge X X.Study on resistance mechanism of soybeans infected by?Phytophthora sojae[D].Harbin:Northeast Agricultural University,2001:45-47.)
相似文献/References:
[1]刘章雄,李卫东,孙石,等.1983~2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(04):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(04):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(04):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(04):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(04):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(04):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(04):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(04):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(04):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(04):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
[11]任龙翚,张宝强,武晓玲,等.大豆种质对大豆疫霉菌株Pm8的抗性分析[J].大豆科学,2010,29(01):77.[doi:10.11861/j.issn.1000-9841.2010.01.0077]
REN Long-hui,ZHANG Bao-qiang,WU Xiao-ing,et al.Resistance of Soybean Germplasm to Phytophthora sojae Pm8[J].Soybean Science,2010,29(04):77.[doi:10.11861/j.issn.1000-9841.2010.01.0077]
[12]孙石,赵晋铭,武晓玲,等.黄淮地区大豆种质对疫霉根腐病的抗性分析[J].大豆科学,2008,27(03):465.[doi:10.11861/j.issn.1000-9841.2008.03.0465]
SUN Shi,ZHAO Jin-ming,WU Xiaoling,et al.Resistance of Soybean Germplasm to Phytohpthora in Huanhuai Valley[J].Soybean Science,2008,27(04):465.[doi:10.11861/j.issn.1000-9841.2008.03.0465]
[13]张淑珍,徐鹏飞,吴俊江,等.栽培大豆种质资源对大豆疫霉根腐病的抗性评价[J].大豆科学,2007,26(06):914.[doi:10.3969/j.issn.1000-9841.2007.06.020]
ZHANG Shu-zhen,XU Peng-fei,WU Jun-jiang,et al.IDENTIFICATION OF RESISTANCE TO PHYTOPHTHORA SOJAE IN SOYBEAN GERMPLASM[J].Soybean Science,2007,26(04):914.[doi:10.3969/j.issn.1000-9841.2007.06.020]
[14]王帅,张子戌,赵洪锟,等.大豆疫霉根腐病抗性QTL的整合及Meta分析[J].大豆科学,2019,38(06):898.[doi:10.11861/j.issn.1000-9841.2019.06.0898]
WANG Shuai,ZHANG Zi-xu,ZHAO Hong-kun,et al.Phytophthora sojae Root Rot Resistance QTL Integration and Meta-analysis[J].Soybean Science,2019,38(04):898.[doi:10.11861/j.issn.1000-9841.2019.06.0898]
备注/Memo
基金项目:国家自然科学基金(31071439,31171577,31101167);黑龙江省杰出青年基金(JC201308);长江后备支持计划;龙江学者基金;哈尔滨市科技创新项目(2012RFQXN011,2012RFXXN019)。