ZHANG Lian-he,YU Fei-yan,DENG Kun,et al.Study on Physiological Characteristics of Selenite Uptake by Soybean Exised Roots[J].Soybean Science,2014,33(04):519-523.[doi:10.11861/j.issn.1000-9841.2014.04.0519]
大豆根系吸收亚硒酸盐的生理特性研究
- Title:
- Study on Physiological Characteristics of Selenite Uptake by Soybean Exised Roots
- 分类号:
- Q945.1
- 文献标志码:
- A
- 摘要:
- 通过生理学和药理学方法，探讨了大豆根系吸收亚硒酸盐的生理特性。结果表明：大豆离体根吸收亚硒酸盐速率随pH升高而降低。pH3.0和8.0时，大豆吸收亚硒酸盐速率随硒含量增加呈比例升高，数据符合直线方程。pH5.0时，大豆吸收亚硒酸盐随硒含量增加而升高直至饱和，数据符合米氏动力学曲线方程。pH3.0时，DNP、NaF和4℃抑制亚硒酸盐吸收的程度分别为15%、19%和23%；pH5.0时，分别达到81%、80%和79%；pH8.0时，分别为16%、8%和11%。进一步研究表明，pH3.0时，HgCl2和AgNO3抑制亚硒酸盐吸收的程度分别为78%和79%；pH5.0时，磷饥饿显著促进亚硒酸盐吸收；pH8.0时，NPPB、9-CA、NPAA、TEACl、NA和DIDS分别抑制亚硒酸吸收的程度为13%、17%、22%、7%、18%和6%。表明大豆根系能通过水通道以被动方式吸收H2SeO3，通过磷转运蛋白以主动方式吸收HSeO -3。
- Abstract:
- Physiological characteristics of selenite uptake by soybean roots was investigated by means of physiological and pharmacological methods.The results showed that the rate of selenite uptake by soybean excised roots declined with an increase of pH.Concentration-dependent kinetics suggested that the rate of selenite uptake increased in proportion to the Se concentration in the absorption solution at pH3.0 and pH8.0.Linear equations were fitted to the data.The rate of selenite uptake increased and followed saturation kinetics with increasing Se concentrations in the absorption solution at pH5.0.The data fitted a Michaelis-Menten saturation curve.The results showed that DNP,NaF and a temperature of 4℃ repressed the rate of selenite uptake by 15%,19% and 23% at pH3.0,by up to 81%,80% and 79% at pH5.0,and by 16%,8% and 11% at pH8.0.Further study suggested that HgCl2 and AgNO3 inhibited selenite uptake by 78% and 79% at pH3.0.Phosphorus starvation increased the rate of selenite uptake by soybean roots at pH5.0.NPPB,9-CA,NPAA,TEACl,NA and DIDS inhibited selenite uptake by 13%,17%,22%,7%,18% and 6% at pH8.0.It demonstrated that selenite was absorbed passively by soybean roots through aquaporins in the form of H2SeO3 at pH3.0,and was absorbed actively through phosphorus transporters in the form of HSeO -3 at pH5.0.
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备注/Memo
基金项目：河南科技大学校校人才基金（09001107）。