[1]李希臣　雷勃钧　卢翠华　钱　华周思君　韩玉琴　刘昭军.外源DN A导入的大豆品种“黑生101”的RAPD初探[J].大豆科学,1999,18(03):230-235.[doi:10.11861/j.issn.1000-9841.1999.03.0230]
　Li XichenLei Bo junLu CuihuaQian HuaZho u Si junHan YuqinLiu Zhao jun.RAPD VERIFICATION OF THE SOYBEAN CULTIVAR "HEISHENG 101"[J].Soybean Science,1999,18(03):230-235.[doi:10.11861/j.issn.1000-9841.1999.03.0230]
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外源DN A导入的大豆品种“黑生101”的RAPD初探

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第18卷 期数: 1999年03期 页码: 230-235 栏目: 出版日期: 1999-08-25
Title:
RAPD VERIFICATION OF THE SOYBEAN CULTIVAR "HEISHENG 101"
作者:
李希臣　雷勃钧　卢翠华　钱　华周思君　韩玉琴　刘昭军
黑龙江省农业科学院生物技术研究中心哈尔滨　150086
Author(s):
Li Xichen　Lei Bo jun　Lu Cuihua　Qian Hua　Zho u Si jun　Han Yuqin　Liu Zhao jun
Bio. Res. Center, Heilongjiang Academy of Agri. Sci . , Harbin , 150086
关键词:
大豆;  黑生101;  RAPD; 分子验证; 花粉管通道
Keywords:
Soy bean;  Hei sheng 101;  RAPD;  Mo lecula r v eri ficatio n;  Pollen tubecha nnel
DOI:
10.11861/j.issn.1000-9841.1999.03.0230
文献标志码:
A
摘要:
黑生101是利用花粉管通道技术育成的第一个大豆品种,利用132个随机引物对黑生101及其供体、受体的DN A进行RAPD扩增,其中3个单引物和2个双引物组合起来,均能在后代黑生101中扩增出与供体相同而与受体不同的DN A片段,证明黑生101中确有供体DN A片段的导入。
Abstract:
Heisheng 101 w as the fi rst soybea n cul tiva r obtained f rom po llen tube cha nnel. Weused RAPD to ampli fy the to tal DN A of Heisheng 101 and it s dono r and recipient by 132ra ndom primers. The DN A polymo rphi sm resul ts of RAPD molecula r verificatio n indicated: Ei ther 3 sing le random primer or 2 do uble primers combina tions can be amplifiedpolymo rphic DN A f ragment s f rom Heisheng 101. The results proved that Hei sheng 101w as t ruly obtained f rom the t ransforma tion prog eny of it s do no r DN A fragment s int roducedinto recipient.

参考文献/References:

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更新日期/Last Update: 2016-12-27