[1]杨守萍　盖钧镒　徐汉卿.大豆雄性不育突变体N J89— 1的遗传学与细胞学鉴定[J].大豆科学,1998,17(01):32-38.[doi:10.11861/j.issn.1000-9841.1998.01.0032]
　Yang ShoupingGai JunyiXu Hanqing.A GENETICAL AND CYTOMORPHOLOGICAL STUDYON THE MALE STERILEMUTANT NJ89- 1 IN SOYBEANS[J].Soybean Science,1998,17(01):32-38.[doi:10.11861/j.issn.1000-9841.1998.01.0032]
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大豆雄性不育突变体N J89— 1的遗传学与细胞学鉴定

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第17卷 期数: 1998年01 页码: 32-38 栏目: 出版日期: 1998-02-20
Title:
A GENETICAL AND CYTOMORPHOLOGICAL STUDYON THE MALE STERILEMUTANT NJ89- 1 IN SOYBEANS
作者:
杨守萍　盖钧镒　徐汉卿
(南京农业大学大豆研究所　南京　210095)
Author(s):
Yang Shouping　Gai Junyi　Xu Hanqing
( Soybean Research Institute , Nanj ing Agricul tural University , N anj ing 210095)
关键词:
大豆;  雄性不育; 突变体;  遗传学;  细胞学
Keywords:
Soy bean;  Male Sterility;  Mutant;  Inheritance;  Cytomo rpholo gy
DOI:
10.11861/j.issn.1000-9841.1998.01.0032
文献标志码:
A
摘要:
花粉发芽实验N J89— 1雄性不育度高达99. 25% 以上且稳定,人工授粉试验N J89— 1的雌性育性正常,说明N J89— 1是一个雄性不育雌性可育突变体。不育株自然授粉后代中育性分离结果表明N J89— 1雄性不育性受单隐性核基因控制。等位性测验表明N J89— 1不育基因与ms1～ ms5均不等位。N J89— 1在败育时期、减数分裂、四分体形成、小孢子壁发生、花药壁发育、花粉粒形态等诸多方面与ms1～ ms6突变体均存在差异,而与st2～ st5突变体却有相似的减数分裂变异如联会异常等,但是N J89— 1的雌性育性正常, st2～ st5突变体的雌性均高度不育。因而N J89— 1是一个既不同于ms类型又不同于st类型的新雄性不育突变体。建议将N J89— 1雄性不育基因符号定为ms7。
Abstract:
Artificial pollinatio n experiment s rev ealed that the female fertility of N J89- 1 w asno rmal, then, N J89- 1 w as a male- sterile female- fertile muta nt. Pollen g erminationex periments show ed that the male steri li ty o f N J89- 1 w as pret ty high and stable wi th apollen g ermination percentage less than 0. 75% . Genetic data led to the conclusion thatthe male steri li ty of N J89- 1 was co nt rolled by a sing le recessiv e nuclear g ene. Alleli smtests indicated that the male sterile g ene of N J89- 1 w as no n- al lelic to the male sterileg enes of ms1, ms2, ms3, ms4 and ms5. The cy tomorpho logical observa tions o n antherand po llen dev elopment show ed that N J89- 1 was di fferent f rom ms1～ ms6 mutants inmany aspect s such as abor tion stag e, meiosis, tet rad fo rmation, pollen w all and antherw all etc. N J89- 1 display ed similar meio tic abnormalities o f asy napsis o r desynapsis tothose o f st2～ st5 mutants, but dif fered f rom st2～ st5 mutants in female fer tili ty, namely, the female of N J89- 1 w as normal , while those of st2～ st5 muta nt s w ere st ronglyimpai red. Therefore, N J89- 1 w as a new male sterile mutant. The male sterile g enesymbol of N J89- 1 w as proposed to be ms7.

参考文献/References:

[1 ]　盖钧镒,胡蕴珠, 陈建民,顾蕴洁, 1980,保存大豆花粉生活力的试验, 作物学报6( 1): 11～ 16

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[3 ]　Alb ert sen, M. C. and R. G. Palmer. 1979. A com parativ e ligh t- and elect ron- mi croscopi c s tud y of microsporog enesis in male s teri le( msl ) and male f ert ile s oybeans ( Gl ycine max ( L. ) Merr. ) . Amer. J. Bot .66( 3): 253- 265
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[5 ]　Delannay, X. and R. G. Palmer. 1982. Genetics and cyt ology of the ms 4 mal e- s terile soyb ean. Th e Journalof Heredi t y 73: 219- 223
[6 ]　Gai Junyi, Yang Shou ping and Xu Hanqing. 1997. The perf ormance and allelism s tudy of the new mal es terile m utan t N J89- 1 of th e soyb ean. Soybean Genetics New sl ett er 24: 57- 59
[7 ]　Grayb os ch , R. A. et al. 1984. Genetic and cytol ogical studies of a mal e- s teri le, f emal e- f erti le s oybeanmu tant. The Journal of Heredit y 75: 383- 388
[8 ]　Hadl ey, H. H. and W. J. St arnes. 1964. Steri li ty in Soybeans Caused by As ynapsis. Crop Sci. 4: 421- 424
[9 ]　Ow en, F. V. 1928. A s teri le charact er in soybeans. Plant Ph ysiology 3: 223- 226
[10 ]　Palm er, R. G. 1974. A Desynapti c Mu tant in the Soybean. Th e Journal of Heredit y 65: 280- 286
[11 ]　Palm er, R. G. , C. W. Joh ns , and P. S. Muir. 1980. Gen eti cs and cytol og y of the ms 3 male- st eril e soybean. The Journal of Heredit y 71: 343- 348
[12 ]　Palmer, R. G. and M. L. H. Kaul. 1983. Genetics, cytol og y, and linkag e s tudi es of a desynaptic s oybeanmut ant. The Jou rnal of Heredi t y 74: 260- 264
[13 ]　Skorups ka, H. and R. G. Palmer. 1989. Genetics and Cytolog y of th e ms 6 Male- S teri le Soybean. Th eJournal of Heredit y 80: 304- 310

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更新日期/Last Update: 2017-01-13