PAN Lin-lin,DUAN Yu-xi,CHEN Li-jie,et al.Screening of Fermentation Conditions for Fungi snef960 to Induce Anti-atrazine in Soybean[J].Soybean Science,2011,30(02):272-276.[doi:10.11861/j.issn.1000-9841.2011.02.0272]
诱导大豆抗阿特拉津青霉snef960菌株摇瓶发酵条件的筛选
- Title:
- Screening of Fermentation Conditions for Fungi snef960 to Induce Anti-atrazine in Soybean
- 文章编号:
- 1000-9841（2011）02-0272-05
- Keywords:
- Soybean; Atrazine; Fungi; Screening; Fermentation conditions
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 从实验室保存菌种中筛选出一株具有诱导大豆抗阿特拉津的青霉菌株snef960。利用正交试验优化了发酵培养基组分，其最佳碳氮比为葡萄糖（4%）、硝酸铵（0.6%），发酵培养基中4种无机盐的最佳配比为：氯化钾（0.04%）、磷酸氢二钾(0.15%) 、硫酸镁(0.04%)、 硫酸亚铁(0.001%)。通过单因素试验确定青霉snef960的最佳摇瓶发酵条件：培养基初始pH值为8，发酵时间为9 d，转速为180 r·min-1，100 mL的三角瓶装液量为70 mL，接种量为10%。
- Abstract:
- Snef 960 which have efficiency to induce anti-atrazine in soybean was screened from laboratory save strains. Cultivation medium of snef960 was researched by orthogonal experiment. The result showed that the optimal C/N ratio was composed of 4% glucose,0.6% NH4NO3,the optimal inorganic salts ratio was composed of 0.04% KCl,0.15% K2HPO4, 0.04% MgSO4·7H2O,0.001% FeSO4.The culture conditions were screened with mono-factor test, and the most suitable technical parameters were summed up as follows: pH8 before sterilized, fementation time 9 d, agitation 180 r·min-1, volume 70/100mL,inoculated volume 10%.
参考文献/References:
[1]赵团结,盖钧镒,李海旺,等.超高产大豆育种研究的进展与讨论[J].中国农业科学,2006,39(1)：29-37. (Zhao T J, Cai J Y, Li H W, et al. Advances in breeding for super high-yielding soybean cultivars[J]. Scientia Agricultura Sinica,2006,39(1)： 29-37.) [2]叶新强,鲁岩,张恒.除草剂阿特拉津的使用与危害[J].环境科学与管理,2006,31(8)：95-97.(Ye X Q, Lu Y, Zhang H. The usage and permiciousness of the herbicide Atrazine[J].Environment Protective Monitoring Station,2006,31(8)：95-97.） [3]化学工业出版社.中国化工产品大全（下卷）：除草剂[M]. 北京：化学工业出版社,1994.（Chemical Industry Press.China chemical products(third volume)：Pesticide[M].Beijing：Chemical Industry Press,1994） [4]王焕民,张子明.新编农药手册[M]. 北京：农业出版社,1989.(Wang H Z,Zhang Z M.New pesticide manual[M].Beijing：Agricultural Press,1989.) [5]武小霞,李文斌,张淑珍，等.我国大豆转基因研究进展[J].大豆科学,2005,24（2）：144-149. (Wu X X, Li W B, Zhang S Z, et al. The research advance on soybean transgene in China[J].Soybean Science,2005,24（2）：144-149.) [6]Strong L C, Rosendahl C, Johnson G, et al. Arthrobacter aurescensTC1 metabolizes diverse s-triazine ring compounds[J]. Applied and Environmental Microbiology, 2002,68(12)：5973-5980. [7]Topp E, Mulbry W M, Zhu H, et al. Characterization of s-triazine herbicide metabolism by a Nocardiodes sp. isolated from agricultural soils [J]. Applied & Environment MicrobiologY, 2000,66(8)：3134-3141. [8]董汉松.植物诱导抗性原理和研究[M].北京：科学出版社，1995.(Dong H S.Theory and research in plant induced resistance[M].Beijing：Science Press,1995.) [9]余龙江.发酵工程原理与技术应用[M].北京：化学工业出版社,2006：52.（Yu L J. Principle and technology application of fermentation process[M]. Beijing：Chemical Industry Press,2006：52.） [10]曲音波.微生物技术开发原理[M].北京：化学工业出版社,2005：102-106.(Qu Y B, Microbial technology development principle[M]. Beijing：Chemical Industry Press, 2005：102-106.)
相似文献/References:
[1]刘章雄,李卫东,孙石,等.1983～2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(02):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(02):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(02):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(02):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(02):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(02):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(02):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(02):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(02):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(02):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
[11]沈红秋,段玉玺,陈立杰,等.不同耐性大豆品种阿特拉津处理后的防御酶反应[J].大豆科学,2011,30(02):259.[doi:10.11861/j.issn.1000-9841.2011.02.0259]
SHEN Hong-qiu,DUAN Yu-xi,CHEN Li-jie,et al.Defense Enzymatic Reaction of Different Tolerant Soybean Varieties after Treated with Atrazine[J].Soybean Science,2011,30(02):259.[doi:10.11861/j.issn.1000-9841.2011.02.0259]
[12]罗在全,段玉玺,王媛媛,等.细菌SnebYK发酵液诱导大豆抗阿特拉津的研究[J].大豆科学,2010,29(02):284.[doi:10.11861/j.issn.1000-9841.2010.02.0284]
LUO Zai-quan,DUAN Yu-xi,WANG Yuan-yuan,et al.Fermentation Broth of Bacteria SnebYK Induced Anti-atrazine Effect in Soybean[J].Soybean Science,2010,29(02):284.[doi:10.11861/j.issn.1000-9841.2010.02.0284]
备注/Memo
基金项目：现代农业产业技术体系岗位科学家专项资助项目。