ZHAO Kun,DONG Shou-kun,LIU Li-jun,et al.Effects of Drought Stress on Physiological Characteristics of Root System of Spring Soybean in Flowering Period[J].Soybean Science,2010,29(03):437-439,443.[doi:10.11861/j.issn.1000-9841.2010.03.0437]
干旱胁迫对春大豆开花期根系生理特性的影响
- Title:
- Effects of Drought Stress on Physiological Characteristics of Root System of Spring Soybean in Flowering Period
- 文章编号:
- 1000-9841(2010)03-0437-03
- Keywords:
- Soybean; Drought stress; SOD; POD; MDA
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 为明确大豆根系生理特性的变化与抗旱性的关系,在盆栽条件下,以3个抗旱性不同的大豆品种为材料,测定开花期根系相对含水率、根系活力、丙二醛含量、超氧化物歧化酶和过氧化物酶活性。结果表明:大豆根系相对含水率及根系活力随干旱胁迫程度增强而降低,抗旱品种降低程度小于不抗旱品种;根系丙二醛含量随干旱程度的增加而升高,各品种间无明显差异;抗旱品种超氧化物歧化酶活性随干旱程度的增加而升高,而不抗旱品种则表现为先升高后降低趋势;过氧化物酶活性各品种间随着干旱胁迫程度的加强均呈先升高后降低趋势,抗旱品种变化更为明显。干旱胁迫下大豆通过改变根系内部的生理变化以提高其抗旱性。
- Abstract:
- The paper aimed to study the relation of drought resistance and the changes of physiological characteristics of soybean root system. It chose three varieties of soybean with different drought resistance potted in pots as experimental samples. The results showed that the relative moisture content and activity of soybean root system reduced with the increasing of drought stress degree, and the reduction level of the variety of soybean with strong drought-resistance was less than the weak one. The content of Malonaldehyde (MDA) of soybean root system rose with increasing the degree of drought, and there was no obvious difference among three varieties of soybean. The activity of Superoxide Dismutase(SOD) in the variety of soybean with strong resistance rose with increasing the level of drought, but the weak one showed increased in the beginning and then decreased. The Peroxidase (POD) activity of all varieties had the tendency of increasing in the beginning period and then decreasing, however, such change appeared in soybean with strong resistance was remarkable. In conclusion, the soybean in the condition of drought stress could increase its drought resistance by changing the physiological characteristics of the root system.
参考文献/References:
[1]Mckersie B D,Leshem Y Y. Stress and stress coping in cultivated plants [M]. Dordecht: Kluwer Academic Publishers,1994:148-180.
[2]刘祖琪,张石诚.植物抗性生理学[M]. 北京: 中国农业出版社, 1994: 101-111.(Liu Z Q,Zhang S C. Plant resistance physiology [M]. Beijing:China Agriculture Press, 1994: 101-111.)
[3]郭卫东,沈向,李嘉瑞,等.植物抗旱分子机理[J]. 西北农业大学学报, 1999, 27(4): 102-106.(Guo W D,Sen X,Li J R. Molecular mechanism of plant drought tolerance [J]. Journal of Northwest Sci-Tech University of Agriculture and Forestry,1999,27(4): 102-106.)
[4]牛明功,王贤,陈龙.干旱、渍涝和低温胁迫对小麦生理生化特性的影响[J]. 种子, 2003(4): 19-21.(Niu G M,Wang X,Cen L. The effect of the stress of drought, waterlogging and low-temperature on wheat physiological and biochemical characteristics [J]. Seed, 2003(4): 19-21.)
[5]Bohnert H J, Jensen R G. Strategise for engineering water-stress tolerance in plant[J]. Trends in Biotechnology, 1996,14: 89-97.
[6]胡新生,王世绩.树木水分胁迫生理与耐旱性研究进展及展望[J]. 林业科学, 1998, 34(2): 77-89.(Hu X S, Wang S J. A review of studies on water stress and drought tolerance in tree species [J]. Scientia Silvae Sinicae, 1998, 34(2): 77-89.)
[7]Rhodenbaugh E J,Pallardy S G. Water stress, photosynthesisand early growth patterns of cuttings of three populus clones[J]. Tree Physiology, 1993, 13(3): 213-226.
[8]张志良,瞿伟菁.植物生理学实验指导(第3版)[M].北京: 高等教育出版社,2003: 267-269.(Zhang Z L,Qu W J. Experimental guidance for plant physiology (The 3rd Version) [M].Beijing: China Higher Education Press, 2003: 267-269.)
[9]李合生.植物生理生化实验原理和技术[M].北京: 高等教育出版社,2000: 164-165,260-261.(Li H S. Experimental principle and technology of plant biochemistry [M]. Beijing: China Higher Education Press, 2000: 164-165, 260-261.)
[10]张宪政.作物生理研究法[M]. 北京: 农业出版社,1992: 206-215.(Zhang X Z. Researc hmethods of crop physiolog[M]. Beijing: China Agriculture Press, 1992:206-215.)
[11]陈晓远,高志红,罗远培,等.不同土壤水分冬小麦根、冠关系及其对叶片水分利用效率的影响[J]. 中国生态农业学报, 2005, 13(2): 134-137.(Chen X Y, Gao Z H, Luo Y P, et al. Relationships between root and shoot of winter wheat under different soil water conditions and their effects on the water use efficiency[J]. Chinese Journal of Eco-Agriculture, 2005, 13(2): 134-137.)
[12]赵世杰.植物组织中丙二醛测定方法的改进[J]. 植物生理学通讯, 1994, 30(3): 207-210.(Zhao S J. The improvement of assay method of malonaldehyde in plant tissue[J]. Plant Physiology Communications, 1994, 30(3): 207-210.)
[13]Mclord J M. Superoxide dismutase: An enzymic function for erythrocuprein(hemocupreim)[J]Journal of Biological Chemistry, 1969, 224: 6049-6055.
[14]刘学师,刘新根,任小林.酸枣根系抗旱性研究[J]. 河北农业大学学报, 2007, 30(6): 34-36.(Liu X S,Liu X G,Ren X L. Study on drought resistance of the root forZiziphus?var. spinosa?Hu [J]. Journal of Agricultural University of Hebei, 2007, 30(6): 34-36.)
[15]王启明,郑爱珍,吴诗光.干旱胁迫对花荚期大豆叶片保护酶活性和膜脂过氧化作用的影响[J]. 安徽农业科学, 2006, 34(8): 1528-1530.(Wang Q M, Zeng A Z, Wu S G. Effects of drought-stress on protective enzyme activity and membrane lipid peroxidation in leaf of soybean flowering-poding period[J]. Journal of Anhui Agricultural Sciences, 2006, 34(8): 1528-1530.)
[16]冯彩平.土壤干旱对冬小麦功能叶过氧化物酶同工酶及抗旱性的影响[J]. 水土保持通报, 1996, 16(4): 46-50.(Feng C P. Effect of soil drought on the peroxidase isoenzyme and drought resistance of winter wheat[J]. Bulletin of Soil and Water Conservation, 1996, 16(4): 46-50.)
相似文献/References:
[1]刘章雄,李卫东,孙石,等.1983~2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(03):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(03):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(03):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(03):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(03):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(03):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(03):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(03):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(03):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(03):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
[11]雍太文,刘小明,肖秀喜,等.不同种子处理对苗期干旱胁迫条件下大豆农艺性状、产量及品质的影响[J].大豆科学,2013,32(05):620.[doi:10.11861/j.issn.1000-9841.2013.05.0620]
YONG Tai-wen,LIU Xiao-ming,XIAO Xiu-xi,et al.Effects of Different Seed Treatments on Agronomic Properties,Yield and Quality of Soybean under Drought Stress at Seedling Stage[J].Soybean Science,2013,32(03):620.[doi:10.11861/j.issn.1000-9841.2013.05.0620]
[12]芮海英,王丽娜,金 铃,等.苗期干旱胁迫对不同大豆品种叶片保护酶活性及丙二醛含量的影响[J].大豆科学,2013,32(05):647.[doi:10.11861/j.issn.1000-9841.2013.05.0647]
RUI Hai-ying,WANG Li-na,JIN Ling,et al.Effect of Drought Stress at Seedling on Protective Enzyme Activity and MDA Content of Different Soybeans[J].Soybean Science,2013,32(03):647.[doi:10.11861/j.issn.1000-9841.2013.05.0647]
[13]王林红,乔 潇,乔亚科,等.PEG模拟干旱胁迫下不同类型大豆的生理生化响应[J].大豆科学,2014,33(03):370.[doi:10.11861/j.issn.1000-9841.2014.03.0370]
WANG Lin-hong,QIAO Xiao,QIAO Ya-ke,et al.Physiological and Biochemical Responses of Different Soybeans under PEG Simulated Drought Stress[J].Soybean Science,2014,33(03):370.[doi:10.11861/j.issn.1000-9841.2014.03.0370]
[14]刘峰,宁海龙,刘剑利,等.干旱胁迫对亚有限大豆植株鲜重建成与分配的影响[J].大豆科学,2011,30(04):609.[doi:10.11861/j.issn.1000-9841.2011.04.0609]
LIU Feng,NING Hai-long,LIU Jian-li,et al.Effects of Drought Stress on Establishment and Distribution of Plant Fresh Weight in Semi-determinate Soybean(Glycine max L.Merill) Varieties[J].Soybean Science,2011,30(03):609.[doi:10.11861/j.issn.1000-9841.2011.04.0609]
[15]阮英慧,董守坤,刘丽君,等.干旱胁迫下外源脱落酸对大豆花期生理特性的影响[J].大豆科学,2012,31(03):385.[doi:10.3969/j.issn.1000-9841.2012.03.010]
RUAN Ying-hui,DONG Shou-kun,LIU Li-jun,et al.Effects of Exogenous Abscisic Acid on Physiological Characteristics in Soybean Flowering under Drought Stress[J].Soybean Science,2012,31(03):385.[doi:10.3969/j.issn.1000-9841.2012.03.010]
[16]董兴月,林 浩,刘丽君,等.干旱胁迫对大豆生理指标的影响[J].大豆科学,2011,30(01):83.[doi:10.11861/j.issn.1000-9841.2011.01.0083]
DONG Xing-yue,LIN Hao,LIU Li-jun,et al.Influence of Drought Stress on Soybean Physiological Indexes[J].Soybean Science,2011,30(03):83.[doi:10.11861/j.issn.1000-9841.2011.01.0083]
[17]刘丽君,林浩,唐晓飞,等.干旱胁迫对不同生育阶段大豆产量形态建成的影响[J].大豆科学,2011,30(03):405.[doi:10.11861/j.issn.1000-9841.2011.03.0405]
LIU Li-jun,LIN Hao,TANG Xiao-fei,et al.Drought Stress Influence Soybean Yield Morphogenesis in Different Growth Stages[J].Soybean Science,2011,30(03):405.[doi:10.11861/j.issn.1000-9841.2011.03.0405]
[18]李建英,田中艳,周长军,等.干旱胁迫下化控种衣剂对大豆幼苗生长发育及保护酶活性的影响[J].大豆科学,2010,29(04):611.[doi:10.11861/j.issn.1000-9841.2010.04.0611]
LI Jian-ying,TIAN Zhong-yan,ZHOU Chang-jun,et al.Effect of Chemical Seed Coating on Growth and Development of Soybean Seedlings under Drought Stress[J].Soybean Science,2010,29(03):611.[doi:10.11861/j.issn.1000-9841.2010.04.0611]
[19]钟鹏,吴俊江,刘丽君,等.低磷和干旱胁迫对不同基因型大豆光合生理特性的影响[J].大豆科学,2009,28(05):806.[doi:10.11861/j.issn.1000-9841.2009.05.0806]
ZHONG Peng,WU Jun-jiang,LIU Li-Jun,et al.Effects of Phosphorus Deficiency and Drought Stress on Photosynthetic Characters in Different Genotypic Soybeans[J].Soybean Science,2009,28(03):806.[doi:10.11861/j.issn.1000-9841.2009.05.0806]
[20]孙海锋,战勇,林海容,等.花期干旱对不同基因型大豆叶绿素荧光特性的影响[J].大豆科学,2008,27(01):56.[doi:10.11861/j.issn.1000-9841.2008.01.0056]
SUN Hai-feng,ZHAN Yong,LIN Hai-rong,et al.Response of Chlorophyll Fluorescence to Drought Stress at Flowering in Different Soybeans[J].Soybean Science,2008,27(03):56.[doi:10.11861/j.issn.1000-9841.2008.01.0056]
备注/Memo
基金项目:国家“十一五”科技支撑计划资助项目(2006BAD21B01-5);东北农业大学博士启动基金资助项目(2009RC57)。