GENG Lei-yue,CUI Shi-you,ZHANG Dan,et al.QTL MAPPING AND EPISTASIS ANALYSIS FOR P-EFFICIENCY IN SOYBEAN [GLYCINE MAX(L.)][J].Soybean Science,2007,26(04):460-466.[doi:10.3969/j.issn.1000-9841.2007.04.003]
大豆磷效率QTL定位及互作分析
- Title:
- QTL MAPPING AND EPISTASIS ANALYSIS FOR P-EFFICIENCY IN SOYBEAN [GLYCINE MAX(L.)]
- 文章编号:
- 1000-9841(2007)04-0460-07
- Keywords:
- Soybean; P-efficiency; RIL; QTL
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 磷效率是复杂的数量性状,有一系列基因参与其中。利用科丰1号×南农1138-2衍生的重组自交系群体NJRIKY,对影响大豆磷效率的加性和上位性QTL同时进行定位,比较在两种磷条件下的大豆磷效率QTL的表达差异。采用5个指标评估大豆的磷效率,包括:茎干重、根干重、根冠比、磷利用效率和磷吸收效率。结果表明:在高磷和低磷条件下在8个连锁群上分别检测到3个和12个加性的QTL,可解释4.0%~13.8%的表型变异;另外,在高磷和低磷条件下,分别检测到12对和7对互作的QTL,可解释3.3%~19.9%的表型变异。本研究的QTL定位结果为进一步了解大豆在不同磷条件下的磷效率遗传机制提供了重要的线索。
- Abstract:
- Phosphorus(P)-efficiency is a complicated quantitative trait,and a number of genes take part in it.This study used the RIL (NJRIKY) derived from Kefeng-1×Nannong1138-2,to map the additive and epistatic QTL which affected P-efficiency and to understand the expressive difference of QTLs associated with P-efficiency under two P conditions.Five P-efficiency related traits were analysed,including shoot dry weight (SDW),root dry weight (RDW),root and shoot ratio (R/S),P use efficiency (PUE) and P absorb efficiency (PAE).Three additive QTLs under high P level and twelve additive QTLs under low P level,were mapped respectively,with 4.0%~13.8% phenotypic variations explained.Epistasis analysis detected nineteen significant additive-by-additive QTLs,twelve under high P level,seven under low P level,with 3.3%~19.9% phenotypic variations explained.This QTL mapping result will provide an important cue to understand the genetic mechanism of P-efficiency in soybean.
参考文献/References:
[1]Abel S,Ticconi C A,Delatorre D A.Phosphate sensing in higher plants[J]Physiol Plant,2002,115:1-8.
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备注/Memo
基金项目:国家重点基础研究发展计划(973计划)项目(2004CB117206);863项目(2006AA10Z1C1);国家自然科学基金重大项目(30490250);教育部长江学者和创新团队发展计划项目(IRT0432)