[1]徐萌,王金缘,胡金丽,等.植物内生菌对大豆促生长和抗胁迫作用的研究进展[J].大豆科学,2017,36(06):965-969,977.[doi:10.11861/j.issn.1000-9841.2017.06.0965]
　XU Meng,WANG Jin-yuan,HU Jin-li,et al.Action of Plant Endophytes on Soybean Growth and Stress Tolerance[J].Soybean Science,2017,36(06):965-969,977.[doi:10.11861/j.issn.1000-9841.2017.06.0965]
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植物内生菌对大豆促生长和抗胁迫作用的研究进展

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第36卷 期数: 2017年06期 页码: 965-969,977 栏目: 出版日期: 2017-11-20
Title:
Action of Plant Endophytes on Soybean Growth and Stress Tolerance
作者:
徐萌; 王金缘; 胡金丽; 崔曼; 李梦雪; 陈熙; 郭晴雪; 马莲菊
（沈阳师范大学 生命科学学院，辽宁 沈阳 110034）
Author(s):
XU Meng;  WANG Jin-yuan;  HU Jin-li;  CUI Man;  LI Meng-xue;  CHEN Xi;  GUO Qing-xue;  MA Lian-ju
(College of Life Science, Shenyang Normal University, Shenyang 110034, China)
关键词:
内生菌;  大豆;  生长发育;  非生物胁迫;  生物胁迫
Keywords:
Plant endophytes;  Soybean;  Growth and development;  Abiotic stress;  Biotic stress
DOI:
10.11861/j.issn.1000-9841.2017.06.0965
文献标志码:
A
摘要:
植物内生菌是指在其生活史或一段生活史在植物体内并对植物无明显病害的一类微生物。内生菌不仅具有自身特有的生存机制，而且对植物的生长发育具有重要作用。随着分子生物学技术的不断进步，人们将分离的植物内生菌转染于大豆植株，通过对大豆在遭受生物和非生物胁迫下叶片光合参数、酶活性、相关基因表达水平及生物量的测定，来分析植物内生菌对大豆生长的影响。本研究综述了植物内生菌的多样性以及近年来植物内生菌在非生物胁迫下如干旱、盐胁迫、重金属等和生物胁迫下如病虫害对大豆生长发育影响的研究进展，并对内生菌应用的前景进行了展望。
Abstract:
Abstract:An endophyte is a bacterium or fungus that lives within a plant and have no obvious disease on the plant. Endophytes not only have their own unique mechanism, but also play an important role on the growth and development of plants. With the continuous progress of molecular biology technology, people isolated plant endophytes and effected soybean plants to study soybean photosynthetic parameters, enzyme activity, related gene expression levels and changes in biomass under abiotic and biotic stress. In this study, we focused on the diversity of plant endophyte and the progress of plant endophytes on soybean growth and development under abiotic stress such as drought, salt stress, heavy metals, etc. and biotic stress such as pests and diseases in recent years Finally, the prospect of the application of endophyte was prospected

参考文献/References:

［1］韩立德, 盖钧镒, 张文明. 大豆营养成分研究现状［J］. 种子, 2003 (5): 58-60. (Han L D, Gai J Y, Zhang W M. Status of soybean nutrient research ［J］. Seed, 2003(5): 58-60.)

［2］宋志华. 大豆苗期病虫害防治技术［J］. 黑龙江科技信息, 2016(19): 267. (Song Z H. Diseases and insect pests in soybean seedling［J］. Heilongjiang Science and Technology Information, 2016 (19): 267.)
［3］孟昭来. 不同类型盐胁迫下宽叶蔓豆（半野生大豆）幼苗生长及光合特性的研究［D］. 长春: 东北师范大学，2013. (Meng Z L. Study on growth and photosynthetic characteristics of seedling (half wild soybean) under different types of salt stress［D］. Changchun: Northeast Normal University, 2013.)
［4］Strobel G, Stierle A, Stierle D, et al. Taxomyces andreana, aproposed new tax on for abulbilliferous by phomycete associated with pacific yew (Taxus brecifolia) ［J］. Mycotaxon, 1993, 47: 71-80.
［5］文才艺, 吴元华, 田秀玲. 植物内生菌研究进展及其存在的问题［J］. 生态学杂志, 2004, 23(2): 86-91. (Wen C Y, Wu Y H, Tian X L. Advances in plant endophytic bacteria and the existing problems［J］. Chinese Journal of Ecology, 2004, 23(2): 86-91.)?
［6］Strobel G A. Endophytes as sources of bioactive products［J］. Microbes & Infection, 2003, 5(6): 535-544.
［7］江军山, 张鑫. 产抗菌活性物质植物内生菌的研究进展［J］. 安徽农业科学, 2010 (22): 11704-11705,11761. (Jiang J S, Zhang X. Advances in research on endophytic bacteria produced by antimicrobial active substances ［J］. Anhui Agricultural Sciences, 2010(22): 11704-11705,11761.)
［8］张琼琼, 黄兴如, 郭逍宇. 湿地植物内生细菌多样性研究进展［J］. 湿地科学, 2015 (2): 233-243. (Zhang Q Q, Huang X R, Guo X Y. Advances in endophytic bacterial diversity of wetland plants ［J］. Wetland Science, 2015(2):233-243.)［9］高增贵, 陈捷, 刘限, 等. 玉米品种遗传多态性与根系内生细菌种群的相互关系［J］. 生态学报, 2006(6): 1920-1925. (Gao Z G, Chen J, Liu X, et al. Relationship between genetic polymorphism of maize varieties and root endophytic bacteria［J］. Journal of Ecology, 2006(6):1920-1925.)
［10］Dalal J, Kulkarni N. Population dynamics and diversity of endophytic bacteria associated with soybean ［Glycine max(L) Merril］［J］. British Microbiology Research Journal, 2013, 3(1): 96-105.
［11］王玉霞, 张淑梅, 赵晓宇, 等. 大豆内生细菌的筛选和鉴定［J］. 大豆科技, 2009 (4): 50-51.(Wang Y X, Zhang S M, Zhao X Y, et al. Screening and identification of endophytic bacteria in soybean［J］. Soybean Science and Technology, 2009 (4): 50-51.)
［12］谭小明, 周雅琴, 陈娟, 等. 药用植物内生真菌多样性研究进展［J］. 中国药学杂志, 2015, 18:1563-1580. (Tan X M, Zhou Y Q, Chen J, et al. Advances in endophytic fungi diversity in medicinal plants ［J］. Chinese Journal of Pharmaceutical and Sciences, 2015, 18: 1563-1580.)
［13］肖佳雷, 姜立超, 尹静, 等. 栽培大豆和野生大豆内生真菌分布及定植特征［J］. 作物杂志, 2015 (4):16-19. (Xiao J L, Jiang L C, Yin J, et al. Distribution and planting characteristics of endophytic fungi in cultivated soybean and wild soybean ［J］. Crop Journal, 2015 (4): 16-19.)
［14］Dalal J M, Kulkarni N S. Population variance and diversity of endophytic fungi in soybean ［Glycine max (L) Merril］［J］. Research and Reviews : Journal of Botanical Sciences, 2014, 3(4):33-39.［15］Sheng Q, Jie L, Chen H H, et al. Isolation, diversity, and antimicrobial activity of rare actinobacteria from medicinal plants of tropical rain forests in Xishuangbanna, China［J］. Applied & Environmental Microbiology, 2009, 75(19):6176-6186.
［16］刘重喜. 大豆根部内生放线菌的筛选、鉴定及其活性代谢产物研究［D］. 长春: 东北农业大学, 2013. (Liu C X. Study on screening, identification and active metabolites of endogenous actinomycetes in soybean root ［D］. Changchun: Northeast Agricultural University, 2013.)
［17］关雪娇. 生防菌NEAU-Da4对大豆内生放线菌的影响及特异放线菌鉴定［D］. 长春: 东北农业大学, 2016. (Guan X J. Effect of NEAU-Da4 on endophytic actinomycetes and identification of specific actinomycetes ［D］. Changchun: Northeast Agricultural University, 2016.)
［18］任安芝, 高玉葆. 植物内生真菌—一类应用前景广阔的资源微生物［J］. 微生物学通报, 2001 (6): 90-93. (Ren A Z, Gao Y B.Plant endophytic fungi - a broad application of resources microbes ［J］. Microbiology Bulletin, 2001(6):90-93.)
［19］赵龙飞, 徐亚军, 常佳丽, 等. 具ACC脱氨酶活性大豆根瘤内生菌的筛选、抗性及促生作用［J］. 微生物学报, 2016(6): 1009-1021. (Zhao L F, Xu Y J, Chang J L, et al. Screening, resistance and promoting growth of endophytic soybean bacteria with ACC deaminase activity ［J］. Journal of Microbiology, 2016(6): 1009-1021.)
［20］Yu J, Yu Z H, Fan G Q, et al. Isolation and characterization of indole acetic acid producing root endophytic bacteria and their potential for promoting crop growth［J］. Journal of Agricultural Science & Technology, 2016, 18(5): 1381-1391.
［21］周怡, 毛亮, 张婷婷, 等. 大豆内生芽孢杆菌的分离和促生菌株的筛选及鉴定［J］. 大豆科学, 2009,28(3): 502-506. (Zhou Y, Mao L, Zhang T T, et al. Isolation of bacillus endophytic bacillus and screening and identification of producing strains ［J］. Soybean Science, 2009,28(3): 502-506.)?
［22］衡楠楠, 陈远学, 徐开未, 等. 大豆根瘤菌SCAUs8的接种效果、促生性及系统发育研究［J］. 微生物学通报, 2016(8): 1708-1714. (Heng N N, Chen Y X, Xu K W, et al. Study on the inoculation effect, proliferation and phylogenetic development of doybean rhizobia SCAUs8 ［J］. Microbiology Bulletin, 2016(8): 1708-1714.)
［23］刘朴方. 农肥和化肥施用对大豆根瘤菌和土壤固氮菌多样性的影响［D］. 长春: 东北农业大学, 2012. (Liu P F. Effects of nongfu and chemical fertilizers on soybean rhizobia and soil nitrogen fixation bacteria diversity ［D］. Changchun: Northeast Agricultural University, 2012.)
［24］孙景宽, 张文辉, 张洁明, 等. 种子萌发期4种植物对干旱胁迫的响应及其抗旱性评价研究［J］. 西北植物学报, 2006, 26(9): 1811-1818. (Sun J K, Zhang W H, Zhang J M, et al. Response of four plants to drought stress and evaluation of drought resistance in seed germination Stage ［J］ . Chinese Journal of Botanical Sciences, 2006, 26(9): 1811-1818.)
［25］王霞, 侯平. 植物对干旱胁迫的适应机理［J］. 干旱区研究, 2001, 18(2): 42-46. (Wang X, Hou P. Plant adaptation mechanism to drought stress ［J］. Research on Arid Land, 2001, 18(2): 42-46.)
［26］曹凯, 李远婷, 安登第, 等. 内生菌对植物抗干旱胁迫能力的影响研究进展［J］. 生物技术通报, 2015(9): 23-29. (Cao K, Li Y T, An D D, et al. Effects of endophytes on plant drought tolerance［J］. Biotechnology Bulletin, 2015(9): 23-29.)
［27］Saravanakumar D, Kavino M, Raguchander T, et al. Plant growth promoting bacteria enhance water stress resistance in green gram plants［J］. Acta Physiologiae Plantarum, 2011,33: 203-209.
［28］Vardharajula S, Ali S Z, Grover M, et al. Drought-tolerant plant growth promoting Bacillus spp : Effect on growth, osmolytes, and antioxidant status of maize under drought stress［J］. Journal of Plant Interactions, 2011, 6: 1-14.
［29］Timmusk S, Wagner E G H. The plant-growth-promoting rhizobacterium Paenibacillus polymyxa induces changes in Arabidopsis thaliana gene expression: Possible connection between biotic and abiotic stress responses［J］. Molecular Plant-Microbe Interactions, 1999, 12(11): 951-959.
［30］Wang Y Q, Ohara Y, Nakayashiki H, et al. Microarray analysis of the gene expression profile induced by the endophytic plant growth promoting rhizobacteria, Pseudomonas fluorescens FPT9601-T5 in Arabidopsis［J］. Molecular PlantMicrobe Interactions, 2005, 18(5): 385-396.
［31］周妍. 盐胁迫对大豆种子萌发、离子平衡及可溶性糖含量影响的研究［D］. 长春: 东北师范大学, 2014. (Zhou Y. Effects of salt stress on soybean seed germination, ion balance and soluble sugar content ［D］. Changchun: Northeast Normal University, 2014).
［32］Leuchtmann A. Systematics, distribution, and host specificity of grass endophytes［J］. Natural Toxins, 1992, 1(2): 150-162.
［33］Li J H, Wang E T, Feng W, et al. Genetic diversity and potential for promotion of plant detected in nodule endophytic bacteria of soybean grown in Heilongjiang province of China［J］. Soil Biology and Biochemistry, 2008, 40(1): 238-246.
［34］吴萍, 何庆元, 李正鹏, 等. 安徽省大豆根瘤菌表型多样性研究［J］. 大豆科学, 2011, 30(2): 219-223. (Wu P, He Q Y, Li Z P, et al. Study on phenotypic diversity of soybean rhizobia in Anhui province ［J］. Soybean Science, 2011, 30(2): 219-223.)
［35］Lyons P C, Evans J J, Bacon C W. Effects of the fungal endophyte Acremonium coenophialum on nitrogen accumulation and metabolism in tall fescue［J］. Plant Physiology, 1990, 92: 726-732.
［36］Radhakrishnan R, Khan A L, Lee I J. Endophytic fungal pre-treatments of seeds alleviates salinity stress effects in soybean plants ［J］. Journal of Microbiology, 2013, 51(6): 850-857.
［37］McInory J A, Koepper J W. Survey of indigenous bacterial endophytes from cotton and sweet corn［J］. Plant and Soil, 1995, 173: 337-342.
［38］Abril A, Zurdo -Pineiro J L, Peix A. Solubilization of phosphate by a strain of Rhizobium leguminosarum bv. trifolii isolated from Phaseolus vulgaris in El Chaco Arido soil (Argentina)［J］. Developments in Plant and Soil Sciences, 2007,102: 135-138.
［39］赵龙飞. 我国大豆根瘤内生菌资源多样性研究［J］. 广东农业科学,2014(9):15-19. (Zhao L F. Study on the diversity of endophytic bacteria in soybean root of China ［J］. Journal of Guangdong Agricultural Sciences, 2014(9): 15-19.)?
［40］Reichman S M.The potential use of the legume-rhizobium symbiosis for the remediation of arsenic contaminated sites［J］. Soil Biology and Biochemistry, 2007, 39: 2587-2593.
［41］Chen W M, Wu C H, James E K, et al. Metal biosorption capability of Cupriavid ustaiwanensis and its effects on heavy metal removal by nodulated Mimosa pudica［J］. Journal of Hazardous Materials, 2008, 151: 364-371.
［42］赵龙飞, 徐亚军, 曹冬建, 等. 溶磷性大豆根瘤内生菌的筛选、抗性及系统发育和促生［J］. 生态学报, 2015，35(13): 4425-4435. (Zhao L F, Xu Y J, Cao D J ,et al. Screening, resistance and phylogeny and growth of endophytic bacteria in soybean root［J］ Journal of Ecology, 2015, 35(13): 4425-4435.)
［43］曹莹, 马宁, 常佳丽, 等. 西北部分矿区豆科植物根瘤菌重金属抗性及16S rDNA RFLP分析［J］.农业环境科学学报, 2010，29(6): 1156-1163. (Cao Y, Ma N, Chang J L, et al. Analysis of heavy metal resistance and 16S rDNA RFLP of rhizobium leguminosae in some areas of northwest China ［J］. Journal of Agricultural Environmental Science, 2010, 29(6): 1156-1163.)
［44］徐亚军. 植物内生菌资源多样性研究进展［J］. 广东农业科学, 2011(24): 149-152. (Xu Y J. Advances in plant endophytic bacteria diversity［J］. Guangdong Agricultural Sciences, 2011(24): 149-152.)
［45］王春, 王芊, 曹旭. 大豆内生菌的分离及菌核病拮抗菌的筛选［J］. 黑龙江农业科学,2015(4):27-30.(Wang C,Wang Q,Cao X. Isolation of soybean endophytic bacteria and screening of antagonistic bacteria against sclerotinia ［J］. Heilongjiang Agricultural Sciences,2015(4):27-30.)
［46］韩祥东, 花美娜, 冯永君, 等. 拮抗大豆疫霉菌植物内生细菌的筛选与鉴定［J］. 大豆科学, 2012, 31(1): 85-91. (Han X D, Hua M N, Feng Y J, et al. Screening and identification of endophytic bacteria against phytophthora sojae［J］. Soybean Science, 2012, 31(1): 85-91.)
［47］台莲梅.大豆根腐病菌（Fusarium oxysporum）毒素及其对大豆根部致病作用的研究［D］. 哈尔滨: 东北农业大学, 2003.(Tai L M. Soybean root rot fungi (Fusarium oxysporum) toxins and its pathogenicity to soybean roots ［D］. Harbin: Northeast Agricultural University, 2003.)
［48］胡云云, 高同国, 张冬冬, 等.大豆根腐病拮抗菌枯草芽孢杆菌8-32抗菌物质性质的初步研究［J］. 湖北农业科学, 2016(4): 917-920. (Hu Y Y. Gao T G, Zhang D D, et al. Preliminary study on antimicrobial substances of bacillus subtilis 8-32 antagonistic bacteria of soybean root rot disease ［J］. Hubei Agricultural Sciences, 2016(4): 917-920.)?
［49］Sharma R D. Effect of Bacillus spp. toxins on oviposition and juvenile hatching of Heterodera glycines［J］. Nematologia Brasileira, 1996.?
［50］史凤玉, 武云鹏, 张瑞敬, 等.野生大豆内生细菌多样性及其杀线虫活性分析［J］. 植物保护学报, 2013(4): 327-332. (Shi F Y, Wu Y P, Zhang R J, et al. Endophytic bacterial diversity and nematocidal activity of wild soybean ［J］. Journal of Plant Protection, 2013(4): 327-332.)
［51］赵宇枢.大豆根瘤内拮抗胞囊线虫细菌筛选及活性菌株初步研究［D］. 沈阳: 沈阳农业大学, 2009. (Zhao Y S. Screening of bacterial nematode bacteria and its active strains in soybean nodules ［D］. Shenyang: Shenyang Agricultural University, 2009.)

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[8]崔喜安.硼钼钴配合施用对大豆产量的影响[J].大豆科学,1999,18(02):173.[doi:10.11861/j.issn.1000-9841.1999.02.0173]
　Cui Xian.INFLUENCE OF B, Mo AND Co COMBINED APPLICATIONON YIELD OF SOYBEAN[J].Soybean Science,1999,18(06):173.[doi:10.11861/j.issn.1000-9841.1999.02.0173]
[9]张梅申沈爱光熊晓辉沈昌.植酸酶对大豆中植酸降解作用的研究[J].大豆科学,1999,18(03):269.[doi:10.11861/j.issn.1000-9841.1999.03.0269]
　Zhang Meishen Shen Ainguang Xiong Xiaohui Shen Chang.LIQUID FERMENTATION OF PHYTASE-PRODUCTION STEAIN[J].Soybean Science,1999,18(06):269.[doi:10.11861/j.issn.1000-9841.1999.03.0269]
[10]许艳丽　李兆林　韩晓增　王守宇　何喜云.壮丰安对大豆生长发育及产量的调控研究[J].大豆科学,1999,18(04):355.[doi:10.11861/j.issn.1000-9841.1999.04.0355]
　Xu YanliLi ZhaolinHan XiaozengWang ShouyuHe Xiy un.EFFECTS O F ZHU ANGFENGAN( ZFA) ON GROWT HREGULATION AND YIELD OF SOYBEAN[J].Soybean Science,1999,18(06):355.[doi:10.11861/j.issn.1000-9841.1999.04.0355]

备注/Memo

基金项目：国家自然科学基金项目（31270369；31600314）；沈阳市科学技术计划项目（F16-205-1-50）。

第一作者简介：徐萌（1994-），女，硕士，主要从事资源与应用微生物学研究。E-mail:269580564@qq.com。
通讯作者：马莲菊（1969-），女，博士，副教授，主要从事资源与应用微生物学研究。E-mail:malianju@163.com。

更新日期/Last Update: 2018-01-28