[1]魏爱丽　白桦　陈云昭　冯文新.盐胁迫下大豆初生叶愈伤组织SOD活性及其同工酶变化的研究[J].大豆科学,1999,18(01):85-88.[doi:10.11861/j.issn.1000-9841.1999.01.0085]
　Wei AiliBai HuaChen YunzhaoFeng Weng xin.STUDIES ON THE CHANGES OF SUPEROXIDE DISMUTASEACTIVITY AND ITS ISOZYME IN PRIMARY LEAVESCALLUS OF SOYBEAN UNDER SALT STRESS[J].Soybean Science,1999,18(01):85-88.[doi:10.11861/j.issn.1000-9841.1999.01.0085]
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盐胁迫下大豆初生叶愈伤组织SOD活性及其同工酶变化的研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第18卷 期数: 1999年01期 页码: 85-88 栏目: 出版日期: 1999-02-25
Title:
STUDIES ON THE CHANGES OF SUPEROXIDE DISMUTASEACTIVITY AND ITS ISOZYME IN PRIMARY LEAVESCALLUS OF SOYBEAN UNDER SALT STRESS
作者:
魏爱丽　白桦　陈云昭　冯文新
山西农业大学农学系　太谷030801
Author(s):
Wei Aili　Bai Hua　Chen Yunzhao　Feng Weng xin
Department of Agronomy , Shanx i Agricul tural University , Taigu 030801
关键词:
盐胁迫;  大豆; 愈伤组织;  SOD;  同工酶
Keywords:
Salt st ress;  Soy bean;  Callus;  SOD;  Isozyme
DOI:
10.11861/j.issn.1000-9841.1999.01.0085
文献标志码:
A
摘要:
以大豆初生叶( Primary leav es)愈伤组织作为材料, 分别于含0% (对照)、0.4%、0. 8%、1. 2% 、1. 6% NaCl浓度的培养基中进行24、48、72小时短时间培养和16、20、24天长时间培养,研究了不同盐度及不同培养时间内SOD活性及其同工酶的变化。结果表明: 短时间培养时,随着盐度的升高和时间的延长, SOD活性增强;长时间培养时,盐浓度越高,胁迫时间越长, SOD活性越低,其同工酶也表现出规律性的变化。
Abstract:
The primary leav es callus o f soybea n w as cultured o n the v arious mediums w hichcontain 0 ( co ntro l) , 0. 4% , 0. 8% , 1. 2% , 1. 6% NaCl for sho rter time, i , e. 24, 48, 72hours, and for long time, i , e. 16, 20, 24 days to study the chang es of SOD activi ty andi ts i so zyme. The rusult s w ere as follow s: When they w ere cultured fo r shor ter time, theactivi ty of SO D w ere hig her wi th the pro long ing of time a nd increase o f sal t co ncent ration.On the co ntrary , when they w ere cultured fo r long time, the result w ere oppo site.Its i so zyme show reg ular chang es.

参考文献/References:

[1 ]　周荣仁, 1984, 植物组织培养在选择耐盐植物方面的研究概况,曲阜师院学报,植物抗盐生理专刊

[2 ]　周荣仁等, 1984, 植物生理学通讯, ( 5): 13- 16
[3 ]　王建华, 1989, 超氧物歧化酶( SOD)在植物逆境和衰老生理中的作用,植物生理学通讯, ( 1): 1- 7
[4 ]　罗广华等, 1984, 大豆和花生种子超氧物歧化酶的同工酶研究,植物生理学报, 10( 2): 175- 179
[5 ]　廖祥儒等, 1997, 玉米素对盐渍下葡萄叶圆片H2 O2 清除系统的影响,植物学报, 39( 7): 641- 646
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更新日期/Last Update: 2016-12-26