[1]徐香玲　邹联沛　刘伟华　李集临.向大豆导入几丁质酶基因的初步研究[J].大豆科学,1999,18(02):101-108.[doi:10.11861/j.issn.1000-9841.1999.02.0101]
　Xu Xiang lingZo u LianpeiLiu WeihuaLi Jilin.A PRELIMINARY STUDY ON TRANSFERRING CHITINASE GENE INTO SOYBEANS[J].Soybean Science,1999,18(02):101-108.[doi:10.11861/j.issn.1000-9841.1999.02.0101]
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向大豆导入几丁质酶基因的初步研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第18卷 期数: 1999年02期 页码: 101-108 栏目: 出版日期: 1999-05-25
Title:
A PRELIMINARY STUDY ON TRANSFERRING CHITINASE GENE INTO SOYBEANS
作者:
徐香玲　邹联沛　刘伟华　李集临
哈尔滨师范大学　150080
Author(s):
Xu Xiang ling　Zo u Lianpei　Liu Weihua　Li Jilin
Harbin Normal Univ ersity 150080
关键词:
Ti质粒;  大豆;  几丁质酶基因;  转基因植株
Keywords:
Ti plasmid;  So ybean;  Chi tinase g ene;  Transferred g ene plant
DOI:
10.11861/j.issn.1000-9841.1999.02.0101
文献标志码:
A
摘要:
以根癌农杆菌的Ti质粒为介导,将抗真菌病的几丁质酶基因,导入黑龙江省栽培大豆- 东农37号,吉林28号等14个品种。从子叶节和下胚轴诱导出丛生芽,并再生植株,经PCR和DN A分子杂交检测,确认为转化植株。用花粉管通道导入法,直接导入几丁质酶基因,共获223粒种子,经PCR和DN A分子杂交检测的113株大豆幼苗中,有7株呈阳性反应,证明几丁质酶基因已导入并整合到大豆的基因组中。
Abstract:
The chi tinase gene w as transferred into cultiva rs Do ng no ng 37, Ji lin 28, et al. w ithTi plasmid. Adventi tious nuds and reg ennera ted plant were inducted f rom co tylendonusnode a nd h ypoco tyl. PCR and DN A do t blo t ting prov ed tha t they w ere t ransferredplant. Puri fied plasmid PBin 437 with chitinase gene was also t ransferred into sev eralsoybeans va ridties by the pol len tube technique and 223 seeds were obtained. Among 113plants g ermina ted f rom these seeds 7 show ed positiv e reaction in PCR and DN A dotblo tting.

参考文献/References:

[1 ]　董立州, 1993, 生物工程进展, 14( 2): 15- 18

[2 ]　刘广阳, 1996, 大豆科学, 15( 4): 353- 356
[3 ]　时焦, 1997, 中国烟草, 4: 47- 50
[4 ]　徐香玲, 1997, 大豆科学, 16( 1): 6- 11
[5 ]　雷勃钧等, 1991, 大豆科学, 10( 1): 58- 62
[6 ]　Brogli e, K. et al: 1991, Trangenti c plants wi tn enhanced resis tan cl t o the fungal path ogen rhizoctonias olani , Science 254: 1194- 1197
[7 ]　Annu, Rev , 1991, Plant ph ysiol , Plaut Mol, Biol , 42: 205- 225

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更新日期/Last Update: 2016-12-26