[1]张梅申沈爱光熊晓辉沈昌.植酸酶对大豆中植酸降解作用的研究[J].大豆科学,1999,18(03):269-273.[doi:10.11861/j.issn.1000-9841.1999.03.0269]
　Zhang Meishen Shen Ainguang Xiong Xiaohui Shen Chang.LIQUID FERMENTATION OF PHYTASE-PRODUCTION STEAIN[J].Soybean Science,1999,18(03):269-273.[doi:10.11861/j.issn.1000-9841.1999.03.0269]
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植酸酶对大豆中植酸降解作用的研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第18卷 期数: 1999年03期 页码: 269-273 栏目: 出版日期: 1999-08-25
Title:
LIQUID FERMENTATION OF PHYTASE-PRODUCTION STEAIN
作者:
张梅申1沈爱光2熊晓辉2沈昌2
1 河北省农林科学院石家庄050051　
2南京农业大学食品科技学院南京210095
Author(s):
Zhang Meishen1 Shen Ainguang2 Xiong Xiaohui2 Shen Chang2
1 Hebei Academy of Agricultural and Forestry Science,Shijiazhuang,050051
2 Food Scientific and Technological College,Nanjing Agricalture University
关键词:
植酸酶;  大豆;  植酸
Keywords:
Phytase; Soybean; Phytic acid
DOI:
10.11861/j.issn.1000-9841.1999.03.0269
文献标志码:
A
摘要:
以植酸钙作为唯一的磷源,从无花果中分离到一株产植酸酶的菌株,利用该菌株液体发酵所得到的粗酶处理脱脂大豆粉, 30℃下反应90℃分钟,植酸(盐)降解率过75% ,处理液中可溶性蛋白质含量由0. 167mg /ml增加到12. 1mg /ml。
Abstract:
With phytate-Ca as the sole phosphorus source of selected medium,a exeracellular phytase-producing strain was screened from the fiuum.Liquid fermentation was studied and a best fermentation medium was obtained,starch 8%,glucose 2%,NaNO31.0%,KH2PO40.01%,KCI0.05%,MgSO40.05%,FeSO40.01% and initial pH5.0.Inoculating maount 10%,filling liquid volum 10%,extracellular phytase production as much as 870.87pu/ml fermentation liquid was obtained at 30C for 90min.,and soluble protein content in treating liquid was increased from0.167mg/ml to 12.1mg/ml.

参考文献/References:

[1 ]　张梅申、熊晓辉、沈爱光, 1996,农牧产品开发, ( 11): 33- 34

[2 ]　Li lian U. Th ompson and Jan e H. yoon. J. 1984, Food Sci. , 49: 1228- 1229
[3 ]　尹光琳、战立克, 1992, 发酵工业全书,北京: 中国医药科技出版社, 534, 539
[4 ]　诸葛健、王正祥, 1994,工业微生物试验技术手册,北京: 中国轻工业出版社, 121- 214
[5 ]　陈祥照, 1957, 微生物通报, ( 7): 213
[6 ]　中国现场统计研究会农业优化组等, 1994,农业正交设计法,北京: 冶金工业出版社
[7 ]　WO94 /03612PC T /F193 /00310
[8 ]　(日)营原洁. 副岛正美著,张旭译, 1981,蛋白质的定量法,北京: 农业出版社, 186- 189
[9 ]　(英) F. G.普里斯特著, 1988,细胞外酶,北京: 科学出版社
[10 ]　吕耀昌、张玉良等著, 1990,农作物子粒中植酸盐离子交换测定方法的研究,作物品种资源, ( 2): 28- 31

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更新日期/Last Update: 2016-12-27