[1]吴 琼,朱荣胜,齐照明,等.大豆油分含量位点主要等位基因挖掘[J].大豆科学,2012,31(04):543-545,551.[doi:10.3969/j.issn.1000-9841.2012.04.007]
　WU Qiong,ZHU Rong-sheng,QI Zhao-ming,et al.Mining Major Alleles of Oil Content Loci in Soybean[Glycine max(L.)Merr][J].Soybean Science,2012,31(04):543-545,551.[doi:10.3969/j.issn.1000-9841.2012.04.007]
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大豆油分含量位点主要等位基因挖掘

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第31卷 期数: 2012年04期 页码: 543-545,551 栏目: 出版日期: 2012-08-25
Title:
Mining Major Alleles of Oil Content Loci in Soybean[Glycine max(L.)Merr]
文章编号:
1000-9841（2012）04-0542-04
作者:
吴 琼1; 朱荣胜2; 齐照明1; 蒋洪蔚3; 杨春明4; 刘春燕3; 胡国华3; 5; 陈庆山1; 5
1．东北农业大学 农学院，黑龙江 哈尔滨150030；
2.东北农业大学 理学院，黑龙江 哈尔滨 150030；
3.黑龙江省农垦科研育种中心，黑龙江 哈尔滨150090；
4.吉林省农业科学院 水稻研究所，吉林 长春 130117；
5.国家大豆工程技术研究中心，黑龙江 哈尔滨150050
Author(s):
WU Qiong1; ZHU Rong-sheng2; QI Zhao-ming1; JIANG Hong-wei3; YANG Chun-ming4; LIU Chun-yan3; HU Guo-hua3; 5; CHEN Qing-shan1; 5
1.College of Agriculture,Northeast Agricultural University,Harbin 150030，Heilongjiang;
2.College of Science,Northeast Agricultural University,Harbin 150030，Heilongjiang;
3.The Crop Research and Breeding Center of Land-Reclamation,Harbin 150090,Heilongjiang;
4.Rice Research Institute of Jilin Academy of Agricultural Sciences,Changchun 130117,Jilin;
5.The National Research Center of Soybean Engineering and Technology,Harbin 150050,Heilongjiang,China
关键词:
大豆; 油分; 位点; 等位基因
Keywords:
Soybean; Oil content; Locus; Allele
分类号:
S565.1
DOI:
10.3969/j.issn.1000-9841.2012.04.007
文献标志码:
A
摘要:
应用241份国内外大豆资源和18个SSR引物，卡方检验及t检验鉴定出3个与大豆油分含量正向相关的重要位点，分别为Satt343，Sat_295和Satt598，每一位点各含1个与油分相关的重要等位基因，分别为Satt343_3，Sat_295_6和Satt598_7，这3个等位基因对油分含量呈正效应，并在分别在引物Satt343和Sat_295附近发现已定位的与油分含量相关的基因。
Abstract:
Increasing oil content in soybean is an important goal for soybean breeding.Using 18 SSR primers，polymorphisms were examined among 241 germplasms and three major loci of oil content were identified in soybean.These include Satt343,Sat_295,and Satt598.Three unique alleles(Satt343_3，Sat_295_6 and Satt598_7)of these loci had positive correlations with oil contents in soybean using Chi-square and T-test analysis.Two candidate genes relevant to oil content were found within the 3 cM region of Satt343 and Sat_295,respectively.These results may be significant for soybean breeding for high oil content.

参考文献/References:

[1]Paterson A H,Lander E S,Hewitt J D,et al.Resolution of quantitative traits into Mendelian factors by using a complete linkage map of restriction fragment length polymorphisms[J].Nature,1988,335:721-726.

[2]Mansur L M,Orf J H,Chase K,et al.Genetic mapping of agronomic traits using recombinant inbred lines of soybean[J].Crop Science,1996,36:1327-1336.

[3]Csanadi G,Vollmann J,Stift G,et al.Seed quality QTLs identified in a molecular map of early maturing soybean[J].Theoretical and Applied Genetics,2001,103:912-919.

[4]张忠臣，战秀玲，陈庆山，等.大豆油分和蛋白性状的基因定位[J].大豆科学，2004，23（2）：81-85.（Zhang Z C,Zhan X L,Chen Q S,et al.QTL mapping of seed oil and protein content of soybean[J].Soybean science,2004,23(2):81-85.）

[5]Qi Z M,Wu Q,Han X,et al.Soybean oil content QTL mapping and integrating with meta-analysis method for mining genes[J].Euphytica,2011,179:499-514.

[6]Kearsey M J,Hyne V.QTL analysis:a simple marker regression approach[J].Theoretical and Applied Genetics,1994,89:698-702.


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备注/Memo

基金项目：国家自然科学基金（30871551）；现代农业产业体系（CARS-04-02A）；国家转基因专项（2011ZX08004-001）。

第一作者简介：吴琼（1987-），女，在读硕士，研究方向为大豆遗传分析及分子辅助育种。E-mail:diana.qq.wu@gmail.com。
通讯作者：陈庆山（1973-），男，博士，教授，研究方向为大豆生物技术。E-mail：qshchen@126.com；胡国华（1951-），男，博士，研究员，研究方向为大豆遗传育种。E-mail：hugh757@vip.163.com。

更新日期/Last Update: 2014-08-18