[1]金明,李天,刘淑萍,等.大豆异黄酮甙元抗脂质过氧化作用的研究[J].大豆科学,2009,28(05):909-912.[doi:10.11861/j.issn.1000-9841.2009.05.0909]
　JIN Ming,LI Tian,LIU Shu-ping,et al.Anti-lipid Peroxidative Effect of Soybean Isoflavone Aglycones[J].Soybean Science,2009,28(05):909-912.[doi:10.11861/j.issn.1000-9841.2009.05.0909]
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大豆异黄酮甙元抗脂质过氧化作用的研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第28卷 期数: 2009年05期 页码: 909-912 栏目: 出版日期: 2009-10-25
Title:
Anti-lipid Peroxidative Effect of Soybean Isoflavone Aglycones
文章编号:
1000-9841(2009)05-0909-04
作者:
金明; 李天; 刘淑萍; 全吉淑
延边大学 医学部，吉林 延吉 133000
Author(s):
JIN Ming; LI Tian; LIU Shu-ping; QUAN Ji-shu
Health Science Center of Yanbian University，Yanji 133000，Jilin，China
关键词:
大豆; 异黄酮; 甙元; 体外; 脂质过氧化
Keywords:
Soybean; Isoflavones; Aglycones; in vitro; Lipid peroxidation
分类号:
R151.2
DOI:
10.11861/j.issn.1000-9841.2009.05.0909
文献标志码:
A
摘要:
从大豆提取大豆异黄酮糖甙，再将其部分水解成其甙元，研究其体外抗氧化能力。用T-AOC方法测定化学模拟体系中的总抗氧化能力，用八木变法测定过氧化脂质(LOOH)含量，用硫代巴比妥酸(TBA)法测定丙二醛(MDA)含量，用分光光度法测定红细胞溶血和肝线粒体膨胀程度。结果表明：大豆异黄酮甙元的体外总抗氧化能力较强，可抑制血浆及肝组织脂质过氧化，减少红细胞溶血和减轻肝线粒体膨胀程度。说明大豆异黄酮甙元具有抗脂质过氧化作用。
Abstract:
Soybean isoflavone glycosides were extracted from soybean hypocotyl，then isoflavone aglycones were prepared by hydrolysis of isoflavone glycosides and their antioxidative effects were investigated in vitro.The total antioxidant activity was detected by T-AOC method，the content of LOOH (lipid hydroperoxide) was measured by Yagi’s method，the content of MDA (malonyldialdehide) was measured by TBA (thiobarbituric acid) assay，the hemolysis of erythrocytes and the swelling of liver mitochondria were detected by spectrophotometric method.The result showed that soybean isoflavone aglycones exerted the high total antioxidative activities in vitro，inhibited the lipid peroxidation in plasma and liver，reduced the hemolysis of erythrocytes and suppressed the swelling of mitochondria.It is suggested that soybean isoflavone aglycones have the anti-lipid peroxidative effects.

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备注/Memo

基金项目：吉林省科技发展计划资助项目（200705428）；国家自然科学基金资助项目（30360113）。

作者简介：金明(1972-)，女，副教授，研究方向为天然活性物质的药理作用研究。E-mail：jinming@ybu.edu.cn。

更新日期/Last Update: 2014-09-18