LI Rong-ji,ZHAO Feng,ZHENG Ke-wei,et al.Alkaline Extraction Technology of Dietary Fiber from Soybean Dregs and Formula Optimization of Soybean-Based High-Fiber Biscuit[J].Soybean Science,2022,41(03):314-322.[doi:10.11861/j.issn.1000-9841.2022.03.0314]
碱浸提豆渣膳食纤维提取工艺及豆基高纤饼干配方优化
- Title:
- Alkaline Extraction Technology of Dietary Fiber from Soybean Dregs and Formula Optimization of Soybean-Based High-Fiber Biscuit
- Keywords:
- bean dregs; dietary fiber; alkaline leaching; hydrolysis; soybean-based high-fiber crisp biscuits; technology; formulation
- 文献标志码:
- A
- 摘要:
- 为高效提取豆渣中的膳食纤维并加工豆基高纤饼干,本研究采用碱浸提法,通过单因素和正交试验探讨碱浓度、碱浸温度、碱浸时间和胰蛋白酶活力对豆渣中膳食纤维提取率的影响。进一步在鸡蛋、黄油、膳食纤维添加量单因素试验基础上进行酥脆值和感官评价响应面分析,优化豆基高纤饼干配方。结果表明:制备膳食纤维的最佳工艺条件为,碱浸温度90 ℃、碱浓度1%、碱浸时间35 min、添加0.25%的500 UI胰蛋白酶,提取率达到83.14%。豆基高纤饼干的最佳配方为:膳食纤维添加量4%、黄油添加量40.30%、鸡蛋添加量25.50%。品评试验证明,该工艺和配方可制得色泽金黄、口感酥松、具有特异豆香的高膳食纤维酥性饼干。最佳配比方案制作出的豆基高纤饼干各指标均满足国家标准,总体符合大众口感,市场潜力较好。豆基高纤饼干水分含量1.85±0.02 g?(100 g)-1,灰分含量1.49±0.03 g?(100 g)-1,粗蛋白含量5.83±0.42 g?(100 g)-1,可溶性膳食纤维含量0.13±0.03 g?(100 g)-1,碳水化合物含量41.67±2.98 g?(100 g)-1。
- Abstract:
- In order to efficiently extract the dietary fiber from soybean dregs and process the soybean-based high-fiber biscuits, this study adopted the alkaline extraction method and discussed the effect on the fiber extraction rate from soybean dregs of alkali concentration, alkali immersion temperature, alkaline immersion time and trypsin activity through single factor and orthogonal experiments. Further, the crispness value and sensory evaluation response surface analysis were carried out to optimize the formula of bean-based high-fiber biscuit on the basis of single factor experiment of adding amount of egg, butter, and dietary fiber. The results showed that the optimal process conditions for preparing dietary fiber were alkaline leaching temperature of 90 ℃, alkaline concentration of 1%, alkaline leaching time of 35 min, 0.25% of 500 UI trypsin, and an extraction rate of 83.14%. The best formula for soy dietary biscuits was dietary fiber 4%, butter 40.30%, and egg 25.50%. The evaluation test proved that this technology and formula could produce high dietary fiber biscuit with golden color, loose taste and special bean flavor. The soybean-based high-fiber biscuit produced by the best ratio scheme can meet the National Standard and the general taste of the public and has good market potential. The moisture content was 1.85 ± 0.02 g? (100 g)-1, ash content was 1.49 ± 0.03 g? (100 g)-1, crude protein content was 5.83 ± 0.42 g? (100 g)-1, soluble dietary fiber content was 0.13 ± 0.03 g? (100 g)-1, and carbohydrate content was 41.67 ± 2.98 g? (100 g)-1.
参考文献/References:
[1]曲鹏宇,李丹,李志江,等. 膳食纤维功能、提取工艺及应用研究进展[J].食品研究与开发, 2018, 39(19): 218-224. (QU P Y, LI D, LI Z J, et al. Research progress on the function, extraction process and application of dietary fiber[J]. Food Research and Development, 2018, 39(19): 218-224.)[2]唐宇, 王旭熙, 余娇娇. 世界大豆生产走势及我国大豆产业复兴策略[J].南方农业,2018,12(31): 88-92. (TANG Y, WANG X X, YU J J. The trend of world soybean production and the rejuvenation strategy of my country′s soybean industry[J]. Southern Agriculture, 2018, 12(31): 88-92.)[3]陈霞, 杨香久, 徐永华, 等. 豆渣膳食纤维制备工艺的研究[J].大豆科学, 2001, 20(2): 128-132. (CHEN X, YANG X J, XU Y H, et al. Study on the preparation technology of soybean dregs dietary fiber[J]. Soybean Science, 2001, 20(2): 128-132.)[4]卞靖. 提升我国大豆产业国际竞争力的对策研究[J]. 中国物价, 2018(9): 62-65. (BIAN J. Research on the counter-measures to improve the international competitiveness of my country′s soybean industry[J].China Prices, 2018(9): 62-65.)[5]张松, 苏永平, 李涛, 等. 膳食纤维的功能特性及在食品领域的研究进展[J].食品研究与开发, 2018, 39(17): 214-218. (ZHANG S, SU Y P, LI T, et al. The functional properties of dietary fiber and its research progress in the field of food[J].Food Research and Development, 2018, 39(17): 214-218.)[6]张世仙,杨春梅,吴金鸿,等.豆渣膳食纤维提取方法及功能研究进展[J].西南师范大学学报(自然科学版), 2009, 34(4): 93-97. (ZHANG S X, YANG C M, WU J H, et al. Research progress on the extraction method and function of soybean dregs dietary fiber[J]. Journal of Southwest Normal University (Natural Science Edition), 2009, 34(4): 93-97.[7]FANG H C, LI J Y, HUO T Y, et al. Novel double crosslinked gels of soybean protein isolates and soluble dietary fiber from soybean coats with their functionalities[J]. Food Hydrocolloids, 2021, 113: 106474.[8]WARDLEISON〖KG(0.45mm〗 M M, GABRIELA I, PAULA V V, et al. Soybean biodiesel purification through an acid-system membrane technology: Effect of oil quali-ty and separation process parameters[J]. Journal of Chemical Technology & Biotechnology,2020,95(7): 1962-1969.[9]齐惠. 挤压—酶法联合制备豆渣水溶性膳食纤维及其性质研究[D]. 哈尔滨: 东北农业大学, 2016. (QI H. Preparation of water-soluble dietary fiber from bean dregs by extrusion-enzymatic method and its properties[D]. Harbin: Northeast Agricultural University, 2016.)[10]杨梦曦, 朱叶, 邓雪盈, 等. 复合酶法提取豆渣膳食纤维的研究[J].食品与机械, 2014, 30(4): 186-189. (YANG M X, ZHU Y, DENG X Y, et al. Research on extraction of dietary fiber from soybean dregs by complex enzyme[J]. Food & Machinery,2014,30(4):186-189.)[11]杜翠. 大豆酶解聚集体的改性及其在植脂奶油中的应用[D]. 广州: 华南理工大学, 2020. (DU C. Modification of soybean enzymatic hydrolysis aggregate and its application in non-dairy cream[D]. Guangzhou: South China University of Technology, 2020.)[12]周利亘, 陈新峰, 王君虹, 等. 大豆多肽复合酶解工艺条件研究[J]. 食品科技, 2005(7): 22-25. (ZHOU L H, CHEN X F, WANG J H, et al. Study on the technological conditions of soybean peptide compound enzymatic hydrolysis[J]. Food Technology, 2005(7): 22-25.)[13]何昕. 大豆蛋白酶解工艺条件的研究[J]. 科技通报, 2000(4): 278-282. (HE X. Study on the technological conditions of soybean proteolysis[J].Science and Technology Bulletin, 2000(4): 278-282.)[14]郭建. 大豆异黄酮提取纯化工艺研究[D].天津大学,2012. (GUO J. Study on extraction and purification technology of soy isoflavones[D].Tianjin University,2012.)[15]和雪. 荞麦饼干加工工艺及品质分析[D]. 晋中: 山西农业大学, 2019. (HE X. The processing technology and quality analysis of buckwheat biscuits[D]. Jinzhong: Shanxi Agricultural University, 2019.)[16]张月. 无糖燕麦麸皮饼干的研制及工艺优化[D].晋中: 山西农业大学, 2017. (ZHANG Y. Development and process optimization of sugar-free oat bran biscuit[D]. Jinzhong: Shanxi Agricultural University, 2017.)[17]李志. 薏米饼干加工关键技术及质量分析研究[D]. 贵阳: 贵州大学, 2017. (LI Z. Research on key technology and quality analysis of barley biscuits processing[D]. Guiyang: Guizhou University, 2017.)[18]EL-SHENAWY M, HUSSIEN A M S, FOUAD M T. Production of biscuits from mixture of tiger nut flour, milk permeate and soft wheat flour[J]. Asian Food Science Journal, 2020: 11-21.[19]匡凤军,刘群,曹倩蕾,等. 质构仪在食品行业中的应用综述[J].现代食品,2020(3):112-115. (KUANG F J, LIU Q, CAO Q L, et al.Summary of application of texture analyzer in food industry[J]. Modern Food, 2020(3): 112-115.)[20]贺丽霞. 基于TA.XT型质构仪的小麦面坯(片) 加工品质评价方法的研究[D]. 杨凌: 西北农林科技大学, 2011. (HE L X. Research on evaluation method of processing quality of wheat noodles (sheets) based on TA.XT texture analyzer[D]. Yangling: Northwest Agriculture and Forestry University, 2011.)[21]AMALRAJ A, VARMA K, JACOB J, et al. Efficacy and safety of a gut health product (Actbiome) prepared by incorporation of asafoetida-curcumin complex onto the turmeric dietary fiber in the management of gut health and intestinal microflora in healthy subjects: A randomized, double-blind, placebo controlled study[J]. Bioactive Carbohydrates and Dietary Fibre, 2021, 26: 100280.[22]陈卫梅. 大豆膳食纤维饼干的研制[J]. 农产品加工, 2006(12): 61-65. (CHEN W M. Development of soybean dietary fiber biscuits[J]. Processing of Agricultural Products, 2006(12): 61-65.)
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备注/Memo
收稿日期:2021-08-26