(XU Wei-jia,LU Jin,GAO Hui-hui,et al.Mapping of Distichous Alternate Phyllotaxis Gene in Cultivated Soybean[J].Soybean Science,2021,40(04):457-465.[doi:10.11861/j.issn.1000-9841.2021.04.0457]
栽培大豆二列状互生叶序基因初步定位
- Title:
- Mapping of Distichous Alternate Phyllotaxis Gene in Cultivated Soybean
- Keywords:
- Soybean; Plant architecture; Distichous alternate phyllotaxis; BSA-Seq; Mutant; Cytokinin
- 文献标志码:
- A
- 摘要:
- 二列状互生叶序表现为所有三出复叶呈平面状排列，为给栽培大豆（Glycine max）二列状互生叶序的形成机理解析提供参考，促进大豆密植条件下株型研究和分子遗传改良，本研究利用大豆品种中品661经EMS诱变获得的二列状互生叶序新种质皖中黄601与中黄13配置杂交组合，调查F5植株株型，利用F5交互互生性状和二列状互生性状分别构建混池，采用BSA-seq方法进行基因定位，并进行GO功能注释分析。结果表明：BSA-seq测序结果与参考基因组平均比对效率为94.30%，平均覆盖深度为38.01×。SNP-index和Indel-index方法关联分析，在14和15号染色体定位到4个候选区域，区段内共包含216个基因，GO分析表明其中4个基因响应细胞分裂素，4个基因响应乙烯，1个基因响应赤霉素，8个基因响应生长素。不同类型二列状互生叶序顶端分生组织和叶节的细胞分裂素含量显著低于交互互生叶序，与交互互生大豆不同的是，二列状互生大豆顶端分生组织的细胞分裂素含量明显低于叶节部位，说明细胞分裂素分布的差异可能是二列状互生叶序形成的重要原因。
- Abstract:
- The distichous alternate phyllotaxis was a plane arrangement of all three compound leaves. In order to provide a reference for the formation mechanism of distichous alternate phyllotaxis in cultivated soybean (Glycine max), and promote the plant type research and molecular genetic improvement of soybean under dense condition, this study investigated a distichous phyllotaxis novel germplasm (Wanzhonghuang 601) obtained by EMS mutagenesis of soybean cultivar Zhongpin 661. We used this mutant in combination with the Zhonghuang 13 configuration, and investigated the type of F5 plants. And then we constructed the mixed pools by using F5 decussate alternate and distichous alternate traits, respectively, and performed the gene mapping by BSA-Seq method, and took on the GO functional annotation analysis. The results showed that, the average comparison efficiency between the BSA-Seq and the reference genome was 94.30%, and the average coverage depth was 38.01×. According to the SNP-index and Indel-index association analysis, four regions were mapped on chromosomes 14 and 15, comprising a total of 216 genes. Through GO analysis, there were five genes responding to cytokinin, four genes responding to ethylene, one gene responding to gibberellin, and eight genes responding to auxin. Meanwhile, the content of CTK in SAM (shoot apical meristem) and leaf segment of distichous phyllotaxis soybean were significantly lower than that of alternate phyllotaxis soybean, as well as the CTK content of SAM in distichous phyllotaxis soybean was lower than leaf segment, however, it was opposite in alternate phyllotaxis soybean. These results indicated that the difference of cytokinin distribution may be an important reason for the formation of distichous alternate phyllotaxis.
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收稿日期：2021-03-08