GU Xin,YANG Xiao-he,YAO Liang-liang,et al.Whole-Genome Sequencing and Analysis of Cercospora Sojina Race 15[J].Soybean Science,2021,40(04):466-475.[doi:10.11861/j.issn.1000-9841.2021.04.0466]
大豆灰斑病菌Race 15的全基因组测序分析
- Title:
- Whole-Genome Sequencing and Analysis of Cercospora Sojina Race 15
- Keywords:
- Cercospora sojina; Race 15; Whole genome sequencing; Gene function annotation; Virulence-associated genes
- 文献标志码:
- A
- 摘要:
- 大豆灰斑病菌新小种Race 15具有强致病力和明显的生理分化现象，可导致传统抗病大豆品种的抗性丧失。为在基因组水平解析其致病机理，本研究对Race 15菌株进行全基因组测序和基因功能注释，并分析其毒力相关基因的代谢途径。结果表明：大豆灰斑病菌Race 15的全基因组de novo测序共产生601 794个PacBio读数，得到6 038 283 778 bp数据，经过组装得到12个contigs，N50长度为4.9 Mb，组装后得到的总序列长度为40.12 Mb。共预测到12 607个编码基因，基因密度约314个·Mb-1，预测得到了200个tRNA，2个sRNA和13个snRNA。共有680个基因在病原与宿主互作数据库中注释，340个基因在碳水化合物酶数据库中注释，777个基因预测为次生代谢物相关基因，此外还有766个基因预测为分泌蛋白。这些与毒力相关的致病基因主要涉及细胞壁分解酶、真菌的形态构成、毒素和色素生物合成等。
- Abstract:
- The new Race 15 of Cercospora sojina has infectivity and physiological race differentiation, which has caused the breakdown of resistance in most soybean cultivars. To analyze its infection mechanism at the genome level, this research adopted the whole-genome sequencing for preliminary genomics research. The results showed that 601 794 high quality reads were generated by PacBio sequencing, covering 6 038 283 778 bp in total, and having a mean length of 10 033 bp and an N50 length of 13 900 bp. The genome of the Race 15 strain of Cercospora sojina (40.12 Mb) consisted of 12 curated contigs, with an 4.9 Mb N50, and 12 607 coding genes were predicted with a gene density of approximately 314 genes per Mb. Additionally, non-coding genes were predicted, with 200 tRNA, 2 sRNA and 13 snRNA genes being predicted in the genome of Race 15. A total of 680 genes were annotated and classified using the PHI database, 340 genes were annotated in the CAZy database, 777 genes were predicted to be related to secondary metabolism and 766 genes were predicted as secretory proteins. These virulence-related pathogenic genes are mainly involved in cell wall degrading enzymes, fungal morphology, toxins and pigment biosynthesis.
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备注/Memo
收稿日期：2021-02-18