[1]张继雨,郭洁,陈峰娜,等.不同世代间大豆粒形相关QTL 的稳定性分析[J].大豆科学,2016,35(04):581-586.[doi:10.11861/j.issn.1000-9841.2016.04.0581]
　ZHANG Ji-yu,GUO Jie,CHENG Feng-na,et al.Stability Analysis of QTL Associated for Seed Shape Traits in Soybean ContentAcross Different Generations[J].Soybean Science,2016,35(04):581-586.[doi:10.11861/j.issn.1000-9841.2016.04.0581]
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不同世代间大豆粒形相关QTL 的稳定性分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第35卷 期数: 2016年04期 页码: 581-586 栏目: 出版日期: 2016-07-20
Title:
Stability Analysis of QTL Associated for Seed Shape Traits in Soybean ContentAcross Different Generations
作者:
张继雨; 郭洁; 陈峰娜; 李文; 赵雪; 韩英鹏; 李文滨; 滕卫丽
?东北农业大学大豆研究所/大豆生物学教育部重点实验室/农业部东北大豆生物学与遗传育种重点实验室，黑龙江哈尔滨150030
Author(s):
ZHANG Ji-yu; GUO Jie; CHENG Feng-na; LI Wen; ZHAO Xue; HAN Ying-peng; LI Wen-bin; TENG Wei-li
Soybean Ｒesearch Institute，Northeast Agricultural University /Key Laboratory of Soybean Biology in Chinese Ministry of Education /Key Laboratory ofSoybean Biology and Breeding /Genetics of Chinese Agriculture Ministry，Harbin 150030，China
关键词:
大豆;  ＲILs;  粒形;  QTL;  稳定遗传
Keywords:
Soybean;  ＲILs;  Seed Shape;  QTL;  Genetic Stability
分类号:
S565. 1
DOI:
10.11861/j.issn.1000-9841.2016.04.0581
文献标志码:
A
摘要:
以东农46 和L-100 构建的重组自交系群体( ＲILs) 为试验材料，利用2013 和2014 年分别在哈尔滨、呼兰、阿城3 个地点共6 个环境的数据对不同世代的遗传群体粒形进行单环境QTL 分析及多环境联合检测。结果表明: 单环境QTL 分析中，检测到13 个与粒形相关的QTL，位于第5、9、12、15 及18 连锁群上，其中粒长QTL 2 个，表型变异贡献率为21. 61% ～ 26. 81%; 粒宽QTL 5 个，表型变异贡献率为7. 28% ～ 18. 38%; 粒厚QTL 6 个，表型变异贡献率为10. 19% ～ 18. 44%。位于Sat_122 ～ Satt052 标记区间的QTL 位点在粒长及粒厚中都被检测到，位于Sat_119 ～ Satt588、Satt192 ～ Satt568 及Sat_401 ～ Satt192 标记区间QTL 位点在粒宽及粒厚中同时被检测到，存在一因多效性。多环境联合分析中，共检测到15 个与粒形相关的QTL。并且有9 个QTL 位点在单环境及多环境联合检测中均被检测到，表明这些QTL 表达较为稳定。因此，本研究获得1 个粒长QTL( Sat_122 ～ Satt052) 、1 个粒宽QTL( Satt192 ～ Satt568) 及2 个粒厚QTL( Satt192 ～ Satt568，Sat_401 ～ Satt192) 为表现较好的稳定QTL。
Abstract:
A ＲILs population derived from a cross between Dongnong 46 and L-100 was used in the experiment. The differentgeneration populations were evaluated in three locations( Harbin，Hulan and Acheng) ，in 2013 and 2014 using single-enviromentQTL analysis and multi-enviroment joint analysis. The results showed that，thirteen QTLs were detected associated withseed shape in single-enviroment QTL analysis，located in 5，9，12，15，and 18 linkage group. Two QTLs were associatedwith seed length，explained 21. 61% － 26. 81% of the phenotypic variation. Five QTLs were associated with seed width，explained7. 28% － 18. 38% of the phenotypic variation. Six QTLs were associated with seed thick，explained 10. 19% －18. 44% of the phenotypic variation. The QTLs located in the marker interval of Sat_122 － Satt052 associated both seed lengthand seed thick. The QTLs located in the marker interval of Sat_119 － Satt588，Satt192 － Satt568 and Sat_401 － Satt192 associatedboth seed width and seed thick，showed pleiotropy. Fifteen QTLs were detected associated with seed shape in multi-enviromentjoint analysis. Nine QTLs were detected in both methods，showed genetic stability.

参考文献/References:

1］ Hyten D L，Pantalone V Ｒ，Sams C E，et al. Seed quality QTL ina prominent soybean population［J］. Theoretical and Applied Genetics，2004，109: 552-561.

[2］ Wilson D O. Storage of orthodox seeds［M］/ /Basra A S. Seedquality: Basic mechanisms，agricultural implications. New York:Food Products Press，1995: 173-208.
［3］ 来永才，李炜，王庆祥，等. 黑龙江省野生大豆高异黄酮新种质创新利用Ⅰ异黄酮含量及与籽粒相关性状的分析［J］. 大豆科学，2006，25( 4) : 414-416. ( Lai Y C，Li W，Wang Q X，et al. Innovation and utilization of new high isoflavone resource ofwild soybean in Heilongjiang Province: I. Analysis of isoflavonecontent and relevant of characters［J］. Soybean Science，2006，25( 4) : 414-416. )
［4］ 梁慧珍，王树峰，余永亮，等. 6 种大豆粒形性状的QTL 定位［J］. 河南农业科学，2008( 9) : 45-51. ( Liang H Z，Wang S F，Yu Y L，et al. ［J］. QTL mapping of 6 seed shape traits in soybean［J］. Henan Agriculture Sciences，2008( 9) : 45-51. )
［5］ 梁慧珍，余永亮，杨红旗，等. 不同环境下大豆荚粒性状的遗传与QTL 分析［J］. 中国农业科学，2012，45 ( 13 ) : 2568-2579. ( Liang H Z，Yu Y L，Yang H Q，et al. Genetic analysisand QTL mapping of pod-seed traits in soybean under different environments［J］. Scientia Agricultra Sinica，2015，45( 13) : 2568-2579. )
［6］ Salas P，Oyarzo-Llaipen J C，Wang D，et al. Genetic mapping ofseed shape in three populations of recombinant inbred lines of soybean(Glycine max L. Merr. ) ［J］. Theoretical and Applied Genetic，2006，113: 1459-1466.
［7］ 刘晓芬. 大豆栽培品种群体粒形性状及百粒重的关联分析［D］. 南京: 南京农业大学，2010. ( Liu X F. Association analysisfor seed shape traits and 100-seed weight in soybean ( Glycinemax L. Merr. ) ［M］. Nanjing: Nanjing Agricultural University，2010. )
［8］ Moongkanna J，Nakasathien S，Novitzky W P，et al. SSＲ markerslinking to seed traits and total oil content in soybean［J］. ThaiJournal of Agricultural Science，2011，44( 4) : 233-241.
［9］ Xu Y，Li H N，Li G J，et al. Mapping quantitative trait loci forseed size traits in soybean( Glycine max L. Merr. ) ［J］. Theoreticaland Applied Genetics，2011，122: 581-594.
［10］ 刘春燕，齐照明，韩冬伟，等. 大豆产量相关性状的多年多点QTL 分析［J］. 东北农业大学学报，2010，41( 11) : 1-9. ( LiuC Y，Qi Z M，Han D W，et al. QTL analysis of yield componentson soybean under different environment［J］. Journal of NortheastAgricultural University，2010，41( 11) : 1-9. )
［11］ 楼巧君，陈亮，罗利军. 三种水稻基因组DNA 快速提取方法的比较［J］. 分子植物育种，2005，3( 5) : 749-752. ( Lou Q J，Chen L，Luo L J. Comparison of three rapid methods of DNA extractionfrom rice［J］. Molecular Plant Breeding，2005，3 ( 5) :749-752. )
［12］ 梁慧珍，余永亮，杨红旗，等. 大豆粒形性状主效QTL、环境互作和上位性检测［J］. 中国农业科学，2013，46 ( 24) : 5081-5088. ( Liang H Z，Wang S F，Yu Y L，et al. Main，environmentallyinteracted and epistatic QTL for seed shape traits in soybean［J］. Scientia Agricultra Sinica，2013，46 ( 24 ) : 5081-5088. )
［13］ 刘顺湖，周瑞宝，喻德跃，等． 大豆蛋白质有关性状的QTL 定位［J］. 作物学报，2009，35( 12) : 2139-2149. ( Liu S H，ZhouＲ B，Yu D Y，et al. QTL mapping of protein related traits in soybean［Glycine max ( L. ) Merr. ］［J］. Acta Agronomica Sinica，2009，35( 12) : 2139-2149. )
［14］ 陈强. 大豆籽粒相关性状QTL 定位分析［M］. 河北: 河北科技师范学院，2014. ( Chen Q. QTL mapping for seed relatedtraits in soybean ( Glycine max L. Merr. ) ［M］. Hebei: HebeiNormal University of Science ＆ Technology，2014. )
［15］ 杨占烈，戴高兴，翟荣荣，等. 多环境条件下超级杂交稻协优9308 重组自交系群体粒形性状的QTL 分析［J］. 中国水稻科学，2013，27( 5) : 482-490. ( Yang Z L，Dai G X，Zhai Ｒ Ｒ，etal. QTL analysis of rice grain shape traits by using recombinant inbredlines from super hybrid rice Xieyou 9308 in Multi-environments［J］. Chinese Journal of Ｒice Science，2013，27( 5) : 482-490. )

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备注/Memo

基金项目: 国家自然科学基金面上项目( 31471517) ; 黑龙江省教育厅科技项目( 10531012) ; 国家现代农业产业技术体系( CAＲS-04-PS04) ;国家“十二五”科技支撑计划( 2011BAD35B06，2013BAD20B04) 。第一作者简介: 张继雨( 1991-) ，女，硕士，主要从事大豆品质性状的分子标记研究。E-mail: 1306915460@ qq． com。通讯作者: 滕卫丽( 1972-) ，女，研究员，硕导，主要从事大豆遗传育种与生物技术研究。E-mail: twlneau@163． com。

更新日期/Last Update: 2016-08-22