[1]杜沛,沈红艺,李中平,等.染料木素联合多不饱和脂肪酸对MCF-7 细胞脂代谢基因表达的影响[J].大豆科学,2016,35(04):587-592.[doi:10.11861/j.issn.1000-9841.2016.04.0587]
　DU Pei,SHEN Hong-yi,LI Zhong-ping,et al.Effects of Genistein Combined Polyunsaturated Fatty Acids on Lipid MetabolicGene Expression of MCF-7 Cells[J].Soybean Science,2016,35(04):587-592.[doi:10.11861/j.issn.1000-9841.2016.04.0587]
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染料木素联合多不饱和脂肪酸对MCF-7 细胞脂代谢基因表达的影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第35卷 期数: 2016年04期 页码: 587-592 栏目: 出版日期: 2016-07-20
Title:
Effects of Genistein Combined Polyunsaturated Fatty Acids on Lipid MetabolicGene Expression of MCF-7 Cells
作者:
杜沛; 沈红艺; 李中平; 刘霞; 陈高敏; 谢燕
( 上海中医药大学公共健康学院，上海201203)
Author(s):
DU Pei; SHEN Hong-yi; LI Zhong-ping; LIU Xia; CHEN Gao-min; XIE Yan
( College of Public Health，Shanghai University of Traditional Chinese Medicine，Shanghai 201203，China)
关键词:
MCF-7 细胞;  染料木素;  多不饱和脂肪酸
Keywords:
MCF-7 cells;  Gentistein;  Polyunsaturated fatty acids
分类号:
Ｒ737. 9
DOI:
10.11861/j.issn.1000-9841.2016.04.0587
文献标志码:
A
摘要:
选用不同浓度的染料木素( GEN) 及二十二碳六烯酸( DHA) 作用于MCF-7 细胞，研究大豆中抗癌成分染料木素与不同比例的多不饱和脂肪酸( PUFAs) 联用，对乳腺癌细胞MCF-7 增殖及脂代谢基因表达情况的影响。根据DHA的有效浓度设定亚油酸( LA) 及LA∶ DHA( 10∶1) 混合PUFAs 作用浓度。将GEN 分别与DHA、LA 及LA∶ DHA( 10∶ 1)联合作用细胞，检测细胞生存率，并通过qPCＲ 方法检测细胞内过氧化物酶体增殖物激活受体γ( PPAＲγ) 、5 － 脂氧合酶( 5-LOX) 、环氧合酶－ 2( COX-2) mＲNA 表达情况。结果表明: 高浓度GEN( 300， 200， 100 μmol·L － 1 ) 及DHA( 400，300， 200 μmol·L － 1 ) 可抑制MCF-7 细胞的增殖，且随着时间增加抑制作用增强。而低浓度GEN( 50， 25 μmol·L － 1 ) 及LA 组及10∶1 组对细胞增殖有轻度促进作用。添加GEN 的G + L 组和G + 10∶1 组细胞的生存率显著降低，同时GEN可降低G + L 组细胞5-LOX mＲNA 的表达量，且GEN 与LA∶ DHA( 10∶1) 联用，增加了细胞内PPAＲγ mＲNA 的表达水平并抑制了COX-2 mＲNA 的表达。试验证实GEN 可抑制高比例ω-6 PUFAs 对乳腺癌细胞增殖的促进作用，其作用机制主要是通过增加PPAＲγ mＲNA 表达，抑制COX-2 mＲNA 表达水平实现的。大豆的抗乳腺癌作用可能是由于GEN与PUFAs 在抑制乳腺癌细胞增殖方面产生了协同效应。
Abstract:
The effect of gentistein which is the anticancer component in soy，combined different ratios polyunsaturated fattyacids( PUFAs) on proliferation and lipid metabolic gene expression of MCF-7 cells was studied. MCF-7 was cultured with differentconcentration gentistein( GEN) and docosahexaenoic acid( DHA) . According to the effect concentration of DHA，we setlinoleic acid( LA) and mixed PUFAs’ concentration，which was 10∶ 1 LA to DHA. Then we combined GEN with DHA，LAand LA∶ DHA ( 10∶1) respectively，texted the survival rate and detect mＲNA expression of peroxisome proliferators-activatedreceptor γ( PPAＲγ) ，5-lipoxygenase ( 5-LOX) and cyclooxygenase-2 ( COX-2) with qPCＲ. The results showed that highconcentration of GEN and DHA could inhibit proliferation of MCF-7 cells and the effect enhanced with time. However，lowconcentration of GEN could promote the cells proliferation mildly. LA and 10∶ 1 PUFAs promote the cells proliferation，butcell’s proliferation was inhibited when we added GEN. GEN could reduce 5-LOX mＲNA level of MCF-7 cell which was underthe effect of LA. Meanwhile it inhibits proliferation of MCF-7 cell through promoting PPAＲγ mＲNA level and inhibiting COX-2 mＲNA level when the cell is cultured with GEN combined 10∶1 LA∶ DHA. GEN can inhibit the promoting effect of high ratioω-6 PUFAs’ proliferation of breast cancer cells. The mechanism is mainly through improving the expression of PPAＲγ mＲNAand inhibiting COX-2 mＲNA. The synergistic effect of GEN and PUFAs on inhibiting the proliferation of breast cancer cells，may be the reason for the anti breast cancer of soy.

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备注/Memo

基金项目: 上海市研究生创新项目( A2-P3550806) ; 上海中医药大学冠生园健康营养基金( D463) ; 上海中医药大学健康营养中医交叉学科研究平台建设基金( Z1301011206) 。第一作者简介: 杜沛( 1990-) ，女，硕士，主要从事大豆异黄酮及脂肪酸的抗乳腺癌研究。E-mail: jirugedp@ sina. com。通讯作者: 沈红艺( 1962-) ，女，博士，研究员，主要从事中医体质与营养干预研究。E-mail: xhhc1503@ sina. com。

更新日期/Last Update: 2016-08-22