[1]陈强,闫龙,雷亚坤,等.大豆籽粒氨基酸含量的QTL分析[J].大豆科学,2015,34(04):597-600,629.[doi:10.11861/j.issn.1000-9841.2015.04.0597]
　CHEN Qiang,YAN Long,LEI Ya-kun,et al.QTL Mapping of Seed Amino Acids Content in Soybean[J].Soybean Science,2015,34(04):597-600,629.[doi:10.11861/j.issn.1000-9841.2015.04.0597]
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大豆籽粒氨基酸含量的QTL分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第34卷 期数: 2015年04期 页码: 597-600,629 栏目: 出版日期: 2015-08-25
Title:
QTL Mapping of Seed Amino Acids Content in Soybean
作者:
陈强; 闫龙; 雷亚坤; 张颖君; 邸锐; 刘兵强; 杨春燕; 张孟臣
河北省农林科学院 粮油作物研究所/国家大豆改良中心石家庄分中心/农业部黄淮海大豆生物学与遗传育种重点实验室/河北省遗传育种重点实验室，河北 石家庄 050035
Author(s):
CHEN Qiang; YAN Long; LEI Ya-kun; ZHANG Ying-jun; DI Rui; LIU Bing-qiang; YANG Chun-yan; ZHANG Meng-chen
Institute of Cereal and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences/Shijiazhuang Branch of National Soybean Improvement Center/Huanghuaihai Key Laboratory of Biology and Genetic Improvement of Seybean, Ministry of Agriculture/Hebei Laboratory of Crop Genetics and Breeding, Shijiazhuang 050035, China
关键词:
大豆; 重组自交系; 氨基酸含量; QTL
Keywords:
Soybean;  RIL;  Amino acid content;  QTL
DOI:
10.11861/j.issn.1000-9841.2015.04.0597
文献标志码:
A
摘要:
以“冀豆12×黑豆”构建的包含186个家系的F8代重组自交系（RIL）群体为材料，分析16种氨基酸含量，采用性状-标记间单向因素方差分析法（ANOVA）、复合区间作图法（CIM）和完备区间作图法（ICIM）对各氨基酸含量的相关QTL进行检测。结果表明：群体中各性状表型均呈现连续性正态分布并超亲分离。3种方法共检测到谷氨酸、精氨酸、丝氨酸、丙氨酸、苯丙氨酸、异亮氨酸、天冬氨酸和赖氨酸含量相关的14个QTL，分布于B1、D1a、D1b、H、I、J和K连锁群中，贡献率为4.91%～17.02%。其中完备区间作图法检测到的苯丙氨酸含量相关QTL qPhe201贡献率最大为17.02%，增效基因来自于黑豆，并且在该标记区间内利用复合区间作图法同时检测到与天冬氨酸、谷氨酸和赖氨酸含量相关的QTL，分别为qAsp20、qGlu20、qLys20。位于B1连锁群中精氨酸含量相关的QTL qArg11在性状-标记间单向方差分析和ICIM两方法中同时检测到，在ICIM中检测到其贡献率为11.79 %,增效基因来自于冀豆12。
Abstract:
A cross of Jidou 12 and Heidou was made to construct a recombinant inbred line of F8 population which has 186 progenies as the experimental material.Correlation between 16 amino acid content were analysis in Shijiazhuang environment ANOVA, CIM and ICIM were used to identify quantitative trait loci The results showed that the distribution of 16 traits was continuous and normal distribution Fourteen QTL were detected across three method about glutamic acid, arginine, serine, alanine, phenylalanine, isoleucine, aspartic acid and lysine, distributed at B1, D1a, D1b, H, I, J and K linkage group, contribution rate from 4.91% to 17.02% qPhe201 had the highest contribution rate (17.02%), detected by ICIM,favorable alleles originate from Heidou There were three QTL qAsp20,qGlu20 and qLys20 detected by CIM in the same marker interval with qPhe201 qArg11 was detected by ANOVA and ICIM, contribution rate was 11.79%, favorable alleles originate from Jidou 12 These results provide a theoretical basis for markerassisted breeding and fine mapping

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备注/Memo

基金项目：国家现代农业产业技术体系（CARS-004-PS06）；国家高技术研究发展计划“863计划”（2012AA101106）；河北省科技支撑计划（14226309D）。

第一作者简介：陈强（1987-），男，硕士，主要从事大豆分子数量遗传与育种研究。E-mail：chenqiangwsm@163.com。
通讯作者：张孟臣（1956-），男，研究员，主要从事大豆遗传育种研究。E-mail：mengchenzhang@hotmail.com。

更新日期/Last Update: 2015-08-31