[1]盛新颖,詹少华,樊洪泓,等.利用SSR分子标记鉴定“梅桥”大豆种质纯度[J].大豆科学,2010,29(02):210-214.[doi:10.11861/j.issn.1000-9841.2010.02.0210]
　SHENG Xin-ying,ZHAN Shao-hua,FAN Hong-hong,et al.Purity Identification of “Meiqiao” Soybean (Glycine max) by SSR Markers[J].Soybean Science,2010,29(02):210-214.[doi:10.11861/j.issn.1000-9841.2010.02.0210]
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利用SSR分子标记鉴定“梅桥”大豆种质纯度

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第29卷 期数: 2010年02期 页码: 210-214 栏目: 出版日期: 2010-04-25
Title:
Purity Identification of “Meiqiao” Soybean (Glycine max) by SSR Markers
文章编号:
1000-9841（2010）02-0210-05
作者:
盛新颖1; 詹少华2; 樊洪泓1; 蔡永萍1; 林 毅1
1.安徽农业大学 生命科学学院，安徽 合肥 230036；
2.皖西学院 化学与生命科学系，安徽 六安 237012
Author(s):
SHENG Xin-ying1;  ZHAN Shao-hua2;  FAN Hong-hong1;  CAI Yong-ping1;  LIN Yi1
1.School of Life Science, Anhui Agricultural University, Hefei 230036；
2.Chemistry and Biology Department, West Anhui University, Liu’an 237012，Anhui，China
关键词:
SSR标记; 大豆; DNA提取; 种质纯度
Keywords:
SSR Markers;  Soybean;  DNA extraction;  Germplasm Purity
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2010.02.0210
文献标志码:
A
摘要:
“梅桥”大豆是安徽省淮北地区农家菜用大豆品种，长年的连续种植导致纯度比较混杂，产量明显下降，利用SSR分子标记技术对92单株“梅桥”大豆种质纯度进行鉴定，以期为梅桥大豆的提纯复壮提供理论依据。结果表明：采用改良的CTAB方法，可以提取高质量的大豆基因组DNA；在优化SSR-PCR反应体系的基础上，筛选出8对SSR引物，平均每个引物得到条带数10.6个，条带的多态性频率为70.9%，梅桥大豆的种质纯度为38.04%。
Abstract:
“Meiqiao”is a vegetable soybean with good quality in Huaibei Anhui Province, as result of continuous cultivation, the purity and yield reduced seriously. In this article, 92 plants were selected to identify the purity using SSR molecular markers so that it could provide theoretical guide for purification and rejuvenation of “Meiqiao” Soybean. The results showed that the high quality soybean genomic DNA was extracted by improved CTAB method, under optimization of SSR-PCR reaction system, eight SSR primers were selected and 10.6 bands were obtained from each one, the rate of polymorphic bands was70.9%, the purity of “Meiqiao” Soybean germplasm was 38.04%.

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备注/Memo

基金项目：安徽高校省级自然科学研究重点资助项目（KJ2008A089）。

第一作者简介：盛新颖（1982），女，硕士，研究方向为大豆分子育种。E-mail: lingjun19850420@126.com。
通讯作者：林毅，教授，博士生导师。E-mail：linyiahau@126.com。

更新日期/Last Update: 2014-09-13