YANG Liu,SHI Chen,TIAN Zhong-yan,et al.Mapping QTL Associated with Resistance to Soybean Cyst Nematode Race 3 in Cultivar Kangxian 2[J].Soybean Science,2010,29(02):215-217.[doi:10.11861/j.issn.1000-9841.2010.02.0215]
大豆胞囊线虫病3号生理小种抗性QTL定位的研究
- Title:
- Mapping QTL Associated with Resistance to Soybean Cyst Nematode Race 3 in Cultivar Kangxian 2
- 文章编号:
- 1000-9841（2010）02-0215-03
- Keywords:
- Soybean; Resistance to cyst nematode; QTLs
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 利用感大豆胞囊线虫病品种合丰25与抗病品种抗线2号杂交获210个F2代群体，利用150对SSR引物，并采用Windows QTL Cartographer V2.1复合区间法对抗大豆胞囊线虫病的基因进行定位。结果表明： 其中有多态性SSR标记为67个，占44.67%；以LOD值大于2.0作为QTL存在的阈值，检测到2个抗大豆胞囊线虫3号生理小种基因相关的QTL：Qscn-1(Satt163~Satt309)，Qscn-2(Sat440~Satt148)，分别定位在MLG G和MLG I上，且遗传贡献率分别为10.1%和7.6%，与SSR标记Satt309和Satt148的遗传距离分别为7.2 cM和5.6 cM。
- Abstract:
- Soybean cyst nematode (Heterodera glycines Ichinohe;SCN) is the primary disease responsible for yield loss of soybean [Glycine max (L.) Merr.]. The objectives of this research were to identify quantitative trait loci (QTLs) associated with SCN resistance using the SSR marker technology. In the study, 210 F2 progeny segregating from a cross between Hefeng 25, a soybean cultivar susceptible to cyst nematode race 3, and Kangxian 2, a soybean cultivar resistance to cyst nematode race 3, were used to screen over 150 SSR markers. A total of 67 SSR markers were distributed in twenty linkage groups. Two QTLs Qscn-1(Satt163～Satt309) and Qscn-2(Sat440-Satt148) relevant to SCN resistance, were detected on MLG G and MLG I when LOD was set as 2.0, and they accounted for 10.1% and 7.6% of the total phenotypic variation, respectively.
参考文献/References:
[1]刘汉起，商绍刚，甄鸿杰，等．黑龙江省大豆孢囊线虫（Heterodera glycines）生理小种分布研究[J]．大豆科学,1995，14（4）：330-333.（Liu H Q,Shang S G,Zheng H J,et al.The distribution of Soybean Cyst Nematode race 3 in Heilongjiang Prinvince[J].soybean science, ,1995，14（4）：330-333.） 成作物, 2004,24(4):230-232.(Wang H,Qiu L J,Chen L J,et al. Research on soybean resistance to Heterodera glycines applying with the method of SSR Markers[J]. Rain Fed Crop, 2004,24(4):230-232.) [3]关荣霞, 常汝镇, 邱丽娟.大豆重要农艺性状的QTL 定位及中国大豆与日本大豆的遗传多样性分析[D].北京: 中国农业科学院, 2004.(Guan R X,Chang R Z,Qiu L J.Maping QTL of main agronomic traits in soybean and analysis of genetic diversity between Chinese and Japanese soybean[D].Beijing:Chinese Academy of Agricutural Sciences,2004.) [4]王彩洁，徐冉，张礼凤，等. 大豆胞囊线虫抗性基因的分子标记研究进展[J]. 大豆科学,2005, 24(3):216-219.（Wang C J,Xu R, Zhang L F,et al.The studies of molecular marker of resisitant genes on soybean cyst nematode[J]. Soybean Science,2005, 24(3):216-219.） [5]陈品三，齐军山，王寿华，等.我国大豆胞囊线虫生理分化动态的鉴定和监测研究[J].植物病理学报, 2001,3(14):336-341.(Chen P S,Qi J S,Wang S H,et al. Studies on identification and monitoring of physiologic variation of Heterodera glycinesin China[J].Acta Phytopathologica Sinica,2001,3(14):336-341.) [6]Keim P, T C Olson, R C Shoemaker. A rapid protocol for isolating soybean DNA [J]. Soybean Genetics Newsletter,1988,15:150-152. [7]Lander E S, Green P, Abrahamson J, et al. Mapmaker: An interactive computer package for constructing genetic linkage maps of experimental and natural population[J]. Genomics, 1987,(1):174-181. [8]Cregan P B, Jarvik T, Bush A L, et al. An integrated genetic linkage map of the soybean genome[J]. Crop Science, 1999,39:1464-1490. [9]田中艳，高国金，周长军，等.大豆胞囊线虫生理小种变异的研究[J].大豆科学,2007,26(2):290-292.(Tian Z Y, Gao G J, Zhou C J, et al. Study on the variation of soybean cyst nematode[J]. Soybean Science，2007,26 (2):290-292.)
相似文献/References:
[1]刘章雄,李卫东,孙石,等.1983～2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(02):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(02):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(02):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(02):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(02):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(02):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(02):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(02):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(02):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(02):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
备注/Memo
基金项目：黑龙江省农业科学院院重点研究资助项目。