WANG Ruo-min,CHEN Jie,XU Jing-ting,et al.Effect of pH and Heat Treatment on Stability of Soybean Peptides[J].Soybean Science,2009,28(06):1058-1061.[doi:10.11861/j.issn.1000-9841.2009.06.1058]
pH值和热处理对大豆肽稳定性的影响
- Title:
- Effect of pH and Heat Treatment on Stability of Soybean Peptides
- 文章编号:
- 1000-9841(2009)06-1058-04
- Keywords:
- Soybean peptides; pH ; Heat treatment; Stability
- 分类号:
- TS214.2
- 文献标志码:
- A
- 摘要:
- 分别采用Folin-酚法、SE-HPLC、火焰原子吸收分光光度法研究了pH值和加热处理对大豆肽的稳定性(包括溶解性、分子量分布、可溶性钙结合量)的影响。结果表明:在pH值3~11时对大豆肽的溶解性和分子量分布均无显著影响,pH值9时大豆肽的钙结合能力最大;高温热处理(温度为80 ℃、100 ℃)对大豆肽的溶解度无显著影响,但都不同程度的增加了大分子(>20 KDa)的相对含量,100 ℃时肽的聚合速度更快;80 ℃加热10 min提高了大豆肽的钙结合能力,热处理温度升高或加热时间延长均会导致钙结合能力的下降。
- Abstract:
- Soybean peptides are capable of binding calcium,but it is still unclear if this ability will be affected in food-processing.In this paper,the effect of pH and heat treatment on stability of soybean peptides(solubility,molecular weights distribution,amount of soluble bind calcium)were studied by Folin-phenol,SE-HPLC and flame atomic absorption spectrometry.The results showed there were no significant differences of the solubility and molecular weights of soybean peptides between pH 3-11,calcium binding capacity of soybean peptides was highest at pH 9;heating treatment(80 ℃and 100 ℃)have no significant influence on the solubility of soybean peptides while increased the content of high molecular weight.The amount of soluble bind calcium of soybean peptides improved in 80 ℃ for 10 min,whereas it decreased by the elevated temperature or prolonged heating time.Results suggest that pH and heat treatment have obvious influence on the stability of soybean peptides,so appropriate conditions should be selected in food processing.
参考文献/References:
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备注/Memo
第一作者简介:王若敏(1978-),女,硕士,现主要从事大豆肽的相关研究。E-mail:wangruomin@yahoo.cn。