[1]张淑珍,靳立梅,徐鹏飞,等.野生大豆接种大豆疫霉根腐病后苯丙氨酸解氨酶（PAL）活性的变化[J].大豆科学,2009,28(06):1044-1048.[doi:10.11861/j.issn.1000-9841.2009.06.1044]
　ZHANG Shu-zhen,JIN Li-mei,XU Peng-fei,et al.Response of PAL Activity to Phytophthora sojae Inoculation in Glycine soja[J].Soybean Science,2009,28(06):1044-1048.[doi:10.11861/j.issn.1000-9841.2009.06.1044]
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野生大豆接种大豆疫霉根腐病后苯丙氨酸解氨酶（PAL）活性的变化

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第28卷 期数: 2009年06期 页码: 1044-1048 栏目: 出版日期: 2009-12-25
Title:
Response of PAL Activity to Phytophthora sojae Inoculation in Glycine soja
文章编号:
1000-9841(2009)06-1044-05
作者:
张淑珍1; 2; 3; 靳立梅3 ; 徐鹏飞3 ; 陈维元4; 吴俊江5 ; 李文滨3 ; 邱丽娟6; 常汝镇6
1.黑龙江省农业科学院 博士后工作站，黑龙江 哈尔滨 150086；
2.东北林业大学 博士后流动站，黑龙江 哈尔滨 150040；
3.东北农业大学 大豆研究所，国家教育部大豆生物学重点实验室，黑龙江 哈尔滨 150030；
4.黑龙江省农业科学院 绥化分院，黑龙江 绥化 152052；
5.黑龙江省农业科学院 大豆研究所，黑龙江 哈尔滨 150086；
6.中国农业科学院 作物科学研究所，北京 100081)

Author(s):
ZHANG Shu-zhen1; 2; 3; JIN Li-mei3; XU Peng-fei3; CHEN Wei-yuan4; WU Jun-jiang5; LI Wen-bin3; QIU Li-juan6; CHANG Ru-zhen6
3.Soybean Research Institute，Northeast Agricultural University,Key Laboratory of National Education Department，Harbin 150030；
4.Suihua Branch of Heilongjiang Academy of Agricultural Sciences，Suihua 152052；
5.Soybean Research Institute of Heilongjiang Academy of Agricultural Sciences，Harbin 150086；
6.Crop Science Institute，Chinese Academy of Agricultural Sciences，Beijing 100081，China
关键词:
大豆疫霉根腐病; 野生大豆; 苯丙氨酸解氨酶（PAL）; 活性变化
Keywords:
Phytophthora root and stem rot; Glycine soja; PAL; Activity change
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2009.06.1044
文献标志码:
A
摘要:
对大豆疫霉根腐病菌胁迫下抗感不同野生大豆品种根、茎、叶中苯丙氨酸解氨酶（PAL）活性的变化规律进行了研究。结果表明：在接种疫霉根腐病菌1号生理小种后，抗病野生大豆根和茎中的PAL活性在病程的大部分阶段比相应对照增加，并且变化的幅度较大，而感病品种相反。抗感野生大豆叶中PAL活性与对照相比变化幅度均较小。
Abstract:
Phytophthora root and stem rot caused by Phytophthora sojae is a destructive disease in soybean production regions all around the world.Some protective enzymes begin to be active when soybean is infected by P.sojae.Of the enzymes，phenylalanine ammonia-lyase（PAL）is a key and rate-limiting enzyme in phenyl propane metabolize pathway which could produce kinds of antimicrobial products.There is abundant wild soybean in China，but there are few reports on the relationship about PAL and resistance of wild soybean to P.sojae.In this paper，4 resistant wild soybeans and 4 susceptible wild soybeans were inoculated with zoospores of P.sojae race 1 as treatment and those inoculated with water as control.The PAL activity in roots，stems，and leaves were measured after inoculation of 0，12，24，36，48，60，and 72 h in the treatment and control respectively.The results indicated that the PAL activity in roots and stems of resistant wild soybeans was higher than that of the control at most of the stages of pathogenetic process，but was lower than that of control for susceptible wild soybean.The change range of PAL activity in leaves of both susceptible and resistant wild soybeans were relatively low compared with that of control.

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备注/Memo

基金项目：黑龙江省农业科学院博士后基金资助项目（LRB06-010）；中国博士后基金资助项目（20060400835）；国家自然科学基金资助项目(30671317，30810103063，30400285)；黑龙江省自然科学基金资助项目（C200814）；黑龙江省新世纪人才培养计划资助项目 (NCET-06-007)；博士后落户黑龙江科研启动基金资助项目(LBH-Q05032)；国家重点基础研究发展计划资助项目（2004CB117203-4）。

第一作者简介：张淑珍（1972-），女，博士，教授，研究方向为大豆抗病遗传育种。E-mail:dnzhangshuzhen@yahoo.com。?
通讯作者：陈维元，研究员。E-mail:chwy14@163.com;吴俊江，副研究员。E-mail:nkywujj@126.com。

更新日期/Last Update: 2014-09-12