CHEN Min,CAI Hui-wu,SHAO Ji-sheng,et al.Extraction of Soybean Protein by Dual-Enzyme hydrolysis Method[J].Soybean Science,2010,29(03):537-539.[doi:10.11861/j.issn.1000-9841.2010.03.0537]
双酶解法提取大豆蛋白的工艺研究
- Title:
- Extraction of Soybean Protein by Dual-Enzyme hydrolysis Method
- 文章编号:
- 1000-9841（2010）03-0537-03
- Keywords:
- Soybean peptide; Enzymatic hydrolysis; Protein content
- 分类号:
- TS214.2
- 文献标志码:
- A
- 摘要:
- 以豆粕粉为原料，加入高效蛋白酶和胰蛋白酶进行水解研究，对反应温度、底物浓度、反应时间、pH值、酶的用量及酶加入间隔的时间等工艺参数进行优化，并分析了大豆多肽的含量及蛋白质的水解度与各影响因素之间的关系。经过优化得到的反应条件为温度45℃，pH 7.5，液固比7∶1，加酶量为高效蛋白酶和胰蛋白酶各0.2g，反应时间8 h，加酶间隔时间3 h。在此条件下，酶解后的蛋白质含量达85%、水解度为17.72%。
- Abstract:
- To extract protein from soybean powder with hydrolysis method by adding efficient protease and trypsinase, the parameters such as the reaction temperature, substrate concentration, reaction time, pH, enzyme dosage and the time interval of adding enzyme were investigated, and the relationship between soybean peptide content, protein hydrolysis degree and the influencing factors were analyzed.The result showed the optimized conditions were: temperature of 45℃, pH 7.5, water added of 600~700 mL, enzyme amount of 0.2g for both efficient protease and trypsinase, reaction time of 8 h, enzyme adding interval of 3 h. Under these conditions, the protein content in the enzyme reaction reached 85%, and the degree of hydrolysis reached 17.72%.
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备注/Memo
基金项目：陕西省科技攻关资助项目（2007K06-07）。