[1]刘焕成,韩英鹏,腾卫丽,等.东北大豆与北美大豆维生素E含量的分析[J].大豆科学,2008,27(06):925-928.[doi:10.11861/j.issn.1000-9841.2008.06.0925]
　LIU Huan-cheng,HAN Ying-peng,TENG Wei-li,et al.Analysis of Vitamin E Content in Soybeans Derived from Northeast China and North America[J].Soybean Science,2008,27(06):925-928.[doi:10.11861/j.issn.1000-9841.2008.06.0925]
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东北大豆与北美大豆维生素E含量的分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第27卷 期数: 2008年06期 页码: 925-928 栏目: 出版日期: 2008-12-25
Title:
Analysis of Vitamin E Content in Soybeans Derived from Northeast China and North America
文章编号:
1000-9841(2008)06-0925-04
作者:
刘焕成; 韩英鹏; 腾卫丽; 李文滨
东北农业大学大豆研究所，大豆生物学教育部重点实验室，黑龙江 哈尔滨 150030
Author(s):
LIU Huan-cheng; HAN Ying-peng; TENG Wei-li; LI Wen-bin
Soybean Research Institute，Key Laboratory of Soybean Biology of Chinese Education Ministry，Northeast Agricultural University，Harbin 150030，Heilongjiang，China
关键词:
大豆; 维生素E; 高效液相色谱; 聚类分析
Keywords:
Soybean; Vitamin E; HPLC; Cluster analysis
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2008.06.0925
文献标志码:
A
摘要:
采用超声波法从大豆籽粒中萃取维生素E，以DIKMA公司生产的反相柱作为固定相，以甲醇作为流动相，流速为1.5 mL.min-1，并采用荧光检测器（Ex=295 nm，Em=330 nm）的高效液相色谱技术，测定了我国东北与北美大豆品种（系）中的α-、γ-、δ-生育酚和维生素E（VE）总含量，并进行比较及聚类分析。结果表明:供试144份大豆材料基于α-、γ-、δ-生育酚和维生素E总含量均可聚成高、中、低3类，我国东北大豆维生素E含量明显高于北美大豆，是丰富的大豆维生素E资源，为大豆高维生素E育种提供参考。
Abstract:
The vitamin E content in soybean from Northeast China and North America that were harvested in the same place and same year were extracted by the method of ultrasonic wave，and the contents of α、γ、δ-tocopherol and total vitamin E were analyzed by HPLC with C18-reverse phase HPLC column as solid phase，and methanol as mobile phase(Flow = 1.5 mL. min-1).The fluorescence detector(Ex = 295nm，Em = 330nm)was used as detector.The results showed that 144 soybean materials based on the contents of α、γ、δ-tocopherol and total vitamin E were all classified in to three class of high，middle and low by cluster analysis.The contents of α、γ、δ-tocopherol and total vitamin E in soybeans from Northeast China were obviously higher than those from North America.

参考文献/References:

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备注/Memo

基金项目：国家高技术研究发展计划（863计划）目标导向资助项目（2006AA10Z1F1）；国家高技术研究发展计划（863计划）重点资助项目（2006AA100104-4）。

作者简介：刘焕成（1978-），男，硕士研究生，研究方向大豆生物技术。

更新日期/Last Update: 2014-10-04